...Hope Hayman DNA Bar Coding: Is Convenient and Accurate for Taxonomy Studies Introduction— Different samples of “wild card” subjects and given subjects (Drosophila melanogaster and the Coquina clams) genomic DNA were acquired and isolated through several steps to amplify the cytochrome c oxidase 1 (CO1) genes. Through comparisons of these wild cards and given subjects mitochondrial CO1 genes with the BLAST library, it was revealed that DNA bar coding is convenient and accurate for taxonomy study. DNA bar coding utilizes the amplification and purification of a specific region of the mitochondrial genome by polymerase chain reactions (PCR). DNA bar coding then uses the PCR products ran in gel electrophoresis to analyze. Materials and Methods— DNA Isolation The wild card specimens were obtained and brought to the lab for DNA isolation. The first step in DNA isolation was to lyse the specimen. The specimens were first homogenized individually in 1.5 mL microtubes. 20 µL of proteinase K was added to each homogenized sample. 200 µL of AL buffer was then added to each sample. The samples were vertexed and incubated at 70oC for 10 minutes. After the incubation, 200 µL of 100% ethanol was added and vortexed again. The next step in DNA isolation is to bind the DNA. The lysate was transferred to a spin column and centrifuged at 8000 rpm for 1 minute, and the flow through was discarded. 500 µL of AW1 buffer was added, then centrifuged at 8000 rpm for 1 minute, and the flow through was...
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.... UNIVERSITY OF DETROIT MERCY College of Business Administration Foundations of Management – 10047 - 01 MBA 5140 – 01 3.0 Credit Hours Fall Term I 2014-2015 Prerequisite : Completion of the MBA Core Meeting Day, Time and Place: Mondays from 6:40- 9:10 PM Room C & F 228 Day One = August 25, 2014 Instructor Information : Instructor: Dr. Thomas Mawhinney, Ph.D. Office: C & F Room 217 Office Hours: Class meeting days 4:00 to 6:00 p.m. & by appointment at other times Phone (text enabled cell): 313 205-7590 Email: Thomas.Mawhinney@udmercy.edu College of Business Information : Emergency Phone: 313 993-1200 Ask for Wendy FAX (U of D, running most of the time): 313 993-1673 UD Mercy Bookstore Textbook Information & Online Purchase Options Follow either link below, first is bookstore “in general” second is for our specific text book: CAUTION and ADVICE : DO NOT use an “international edition” of this text book or any other edition unless it is THIS text in paperback or electronic format. The campus bookstore will have the exact text we will use in class. There is a good chance that if you use a different edition, e.g., international edition, it will differ in some important ways from the text described above. Please use the following: Title: Organizations: Behavior, Structure, Processes Fourteenth Edition Authors: James L. Gibson, John M. Ivancevich, James H. Donnelly, Jr., Robert Konopaske Publisher: Published by McGraw - Hill, Copyright © 2012 ISBN 978-0-07- 811266 -9 (...
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...El teletrabajo es una nueva tendencia de trabajo que se está implementando alrededor del mundo. Su concepto principal se encuentra en el artículo 2 de la Ley 1221 del 2008: ‘’ Definiciones. Para la puesta en marcha de la presente ley se tendrán las siguientes definiciones: Teletrabajo. Es una forma de organización laboral, que consiste en el desempeño de actividades remuneradas o prestación de servicios a terceros utilizando como soporte las tecnologías de la información y la comunicación – TIC para el contacto entre el trabajador y la empresa, sin requerirse la presencia física del trabajador en un sitio específico de trabajo’’ . En varios países este nuevo concepto ha sido bastante útil, ayudando a que las personas encuentren un nuevo camino para poder trabajar. Se cree que es muy coherente por varios motivos. Primero existe una coherencia jurídica donde, por ejemplo, el teletrabajador tendrá las mismas seguridades y garantías que el trabajador. En el artículo 6 de la Ley 1221/08 se habla de las garantías laborales, sindicales y de seguridad social para los teletrabajadores. Se busca una igualdad entre los trabajadores y los teletrabajadores en todos los campos como lo dispone de igual manera el artículo 4 del Decreto 0884 del 2012 y también el uso del Código Sustantivo del Trabajo en el artículo 39 donde el contrato con un teletrabajador tiene que cumplir con los requisitos dispuestos en este. La segunda es una coherencia social y global. La sociedad cada días más está...
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...Names of all the 60 Tamil years No. Tamil Year Pashali Year Kollam Year Thiruvalluvar year Gregorian Year 01 Prabhava 1396-1397 1162-1163 2018 1987 - 1988 02 Vibhava 1397-1398 1163-1164 2019 1988 - 1989 03 Sukla 1398-1399 1164-1165 2020 1989 - 1990 04 Pramodhoodha 1399-1400 1165-1166 2021 1990 - 1991 05 Prachorpaththi 1400-1401 1166-1167 2022 1991 - 1992 06 Angirasa 1401-1402 1167-1168 2023 1992 - 1993 07 Srimukha 1402-1403 1168-1169 2024 1993 - 1994 08 Bhava 1403-1404 1169-1170 2025 1994 - 1995 09 Yuva 1404-1405 1170-1171 2026 1995 - 1996 10 Thadhu 1405-1406 1171-1172 2027 1996 - 1997 11 Eesvara 1406-1407 1172-1173 2028 1997 - 1998 12 Vehudhanya 1407-1408 1173-1174 2029 1998 - 1999 13 Pramathi 1408-1409 1174-1175 2030 1999 - 2000 14 Vikrama 1409-1410 1175-1176 2031 2000 - 2001 15 Vishu 1410-1411 1176-1177 2032 2001 - 2002 16 Chitrabanu 1411-1412 1177-1178 2033 2002 - 2003 17 Subanu 1412-1413 1178-1179 2034 2003 - 2004 18 Tharana 1413-1414 1179-1180 2035 2004 - 2005 19 Parthiba 1414-1415 1180-1181 2036 2005 - 2006 20 Viya 1415-1416 1181-1182 2037 2006 - 2007 21 Sarvajith 1416-1417 1182-1183 2038 2007 - 2008 22 Sarvadhari 1417-1418 1183-1184 2039 2008 - 2009 23 Virodhi 1418-1419 1184-1185 2040 2009 - 2010 24 Vikruthi 1419-1420 1185-1186 2041 2010 - 2011 25 Kara 1420-1421 1186-1187 2042 2011 - 2012 26 Nandhana 1421-1422 1187-1188 2043 2012 - 2013 27 Vijaya 1422-1423 1188-1189 2044 2013 - 2014 28 Jaya 1423-1424 1189-1190 2045 2014 - 2015 29 Manmatha...
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...Alaska 2013 BAH Rates - WITH DEPENDENTS MHA E-1 1770 1665 1908 1899 1698 1626 E-2 1770 1665 1908 1899 1698 1626 E-3 1770 1665 1908 1899 1698 1626 E-4 1770 1665 1908 1899 1698 1626 E-5 1983 1971 2391 2103 2097 2025 E-6 1992 1980 2400 2112 2106 2034 E-7 2061 2076 2433 2247 2238 2127 E-8 2148 2190 2478 2403 2391 2235 E-9 2271 2319 2592 2568 2577 2358 W-1 1995 1983 2403 2115 2109 2037 W-2 2097 2124 2451 2310 2301 2172 W-3 2205 2265 2508 2502 2490 2307 W-4 2298 2337 2625 2592 2610 2379 W-5 2406 2427 2763 2694 2754 2463 O1E 2079 2100 2442 2277 2271 2148 O2E 2190 2244 2499 2475 2463 2286 O3E 2313 2352 2646 2607 2634 2391 O-1 1986 1974 2394 2106 2100 2028 O-2 1989 1977 2397 2109 2103 2031 O-3 2202 2259 2505 2496 2481 2301 O-4 2448 2460 2817 2736 2808 2496 O-5 2622 2601 3042 2904 3039 2637 O-6 2649 2628 3072 2931 3069 2661 O-7 2673 2652 3105 2961 3102 2688 Alaska KETCHIKAN, AK SITKA, AK JUNEAU, AK KODIAK ISLAND, AK ANCHORAGE, AK FAIRBANKS, AK AK400 AK401 AK402 AK403 AK404 AK405 Alabama 2013 BAH Rates - WITH DEPENDENTS MHA E-1 861 E-2 861 E-3 861 E-4 861 E-5 915 E-6 1020 E-7 1053 E-8 1089 E-9 1170 W-1 1023 W-2 1068 W-3 1113 W-4 1191 W-5 1281 O-1E 1059 O-2E 1107 O-3E 1206 O-1 927 O-2 1017 O-3 1110 O-4 1317 O-5 1464 O-6 1479 O-7 1494 Alabama ANNISTON / FT MCCLELLAN FORT RUCKER HUNTSVILLE, AL MOBILE, AL MONTGOMERY, AL AUBURN, AL BIRMINGHAM, AL TUSLOOSA, AL AL001 AL002 AL003 AL004 AL005 AL006 AL007 AL008 AL001 939 1023 1002 1062 1026 1017 1068 861 939 1023 1002...
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...ADVANCED TAX Consider the following examples designed to illustrate the point that you should study the law and not problems. General rule on income: All income is taxable unless something in the tax law says that it is not. General rule on deductions: Nothing is deductible unless some provision in the tax law says that it is. General rule on losses: The only losses that are deductible are for 1) Investment, 2) Business, or 3) Casualty. Example #1: Bill is in love and buys his girlfriend, Hillary, an engagement ring at a cost of $100. Later, she notices his roving eye and returns the ring to him and he sells it for $95. What is the tax result? (Megan and Phuong) SP = 95, basis = 100 ( loss = The loss is not deductible because the only losses that are deductible are for investment, business or casualty. Example #2: Bill is in love and buys his girlfriend, Hillary, an engagement ring at a cost of $100. Later, she notices his roving eye and returns the ring to him and he sells it for $105. What is the tax result? (Phuong and Jake) The gain of $5 is taxable because nothing says it’s not HEAD OF HOUSEHOLD – The requirements are as follows: 1. The person must be unmarried. 2. The person must a) provide at least half the cost of a household in which at least one b) dependent c) relative lives. Note that in the case of a dependent parent, the parent need not live with the taxpayer. EXAMPLE #3: Justin is single, lives alone, and fully supports...
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...Chapter 1 Problems 1-1 through 1-4 are for student research. 1-5 (a) Point vehicles v x Q= Seek stationary point maximum cars v 42.1v − v 2 = = hour x 0.324 dQ 42.1 − 2v =0= ∴ v* = 21.05 mph dv 0.324 Q* = (b) 42.1(21.05) − 21.052 = 1368 cars/h Ans. 0.324 v l 2 x l 2 v = Q= x +l Maximize Q with l = 10/5280 mi v 22.18 22.19 22.20 22.21 22.22 0.324 l + 2 v(42.1) − v v −1 Q 1221.431 1221.433 1221.435 ← 1221.435 1221.434 1368 − 1221 = 12% 1221 Ans. % loss of throughput = (c) % increase in speed 22.2 − 21.05 = 5.5% 21.05 Modest change in optimal speed Ans. 2 Solutions Manual • Instructor’s Solution Manual to Accompany Mechanical Engineering Design 1-6 This and the following problem may be the student’s first experience with a figure of merit. • Formulate fom to reflect larger figure of merit for larger merit. • Use a maximization optimization algorithm. When one gets into computer implementation and answers are not known, minimizing instead of maximizing is the largest error one can make. FV = F1 sin θ − W = 0 FH = −F1 cos θ − F2 = 0 From which F1 = W/sin θ F2 = −W cos θ/sin θ fom = −$ = −¢γ (volume) . = −¢γ(l1 A1 + l2 A2 ) W F1 l1 = , l2 = A1 = S S sin θ cos θ A2 = W cos θ F2 = S S sin θ l2 W cos θ l2 W + cos θ S sin θ S sin θ 1 + cos2 θ cos θ sin θ fom = −¢γ = Set leading constant to unity θ◦ 0 20 30 40 45 50 54.736 60 −¢γ W l2 S fom −∞ −5.86 −4.04 −3.22 −3.00 −2.87 −2.828 −2.886 θ* = 54.736◦ fom* = −2.828 Alternative: Ans. d 1...
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...Lab #1 Solutions Mathew Arndt Desktop Computer Sales at a Computer Store for One Week Sale # 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 Price 1201 1064 1174 1086 1197 1065 1133 1075 1051 1190 1183 1119 1128 1155 1229 1228 1047 1113 1222 1159 1079 1216 1115 1049 1119 1160 1172 1193 1105 1241 1141 1183 1148 1078 1180 1194 1192 1093 1102 1030 1184 1245 1076 Store South North South Downtown Downtown Downtown North Downtown Downtown South South Downtown North North South North South North North Downtown Downtown South South Downtown North North North North North North South North South South North North Downtown North South South South Downtown Downtown Sell Time 20 20 35 30 30 35 35 30 35 40 30 45 20 15 30 20 25 35 35 30 25 15 30 25 30 20 35 20 35 40 30 20 30 20 25 35 15 40 10 25 30 25 30 Manufacturer Compaq Compaq Gateway Acer Hewlet Packard Gateway Gateway Gateway Compaq Hewlet Packard Compaq Gateway Compaq Compaq Hewlet Packard Compaq Gateway Compaq Hewlet Packard Compaq Hewlet Packard Compaq Gateway Acer Hewlet Packard Compaq Compaq Compaq Acer Gateway Gateway Acer Compaq Gateway Gateway Gateway Gateway Compaq Compaq Acer Compaq Gateway Acer Salesperson Ann Gerardo Ann Simon Lucinda Simon Gerardo Isaac Lucinda Dominick Dominick Isaac Kristof Gerardo Dominick Kristof Ralph Blake Blake James Isaac Dominick Ralph Simon Gerardo Kristof Gerardo Norm Gerardo Gerardo Ralph Blake Ralph Ralph...
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...the concepts be sure that everything is in your own words! If you have any questions please don’t hesitate to ask! ------------------------------------------------------------------------------------------------------- Unit 4 Discussion board: Descriptive statistics Identify your data The data set that I am using is data set one male health exam. The quantitative variable you will be analyzing is cholesterol. Explain the variable The selected variable measures the Cholesterol (in MG) of each male in the exam. Present descriptive statistics Summary statistics: Column | n | Mean | Variance | Std. dev. | Std. err. | Median | Range | Min | Max | Q1 | Q3 | CHOL | 40 | 395.225 | 85504.64 | 292.41177 | 46.23436 | 282.5 | 1221 | 31 | 1252 | 155.5 | 625.5 | Summary statistics: Column | n | Mean | Variance | Std. dev. | Std. err. | Median | Range | Min | Max | Q1 | Q3 | WT | 40 | 172.55 | 693.11949 | 26.327163 | 4.1626899 | 169.95 | 117.6 | 119.5 | 237.1 | 152.2 | 190.1 | Identify best statistic Which measure of central tendency (mean, median or mode) do you think best describes the data set? Fully explain your answer. Explain standard deviation Explain what the standard deviation tells us about variation in our data. ------------------------------------------------------------------------------------------------------------ **Remember to post substantial replies to at least two of your peers. You may find guidelines for acceptable response...
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...KAUNO TECHNOLOGIJOS UNIVERSITETAS EKONOMIKOS IR VADYBOS FAKULTETAS MUZIKINIS KLUBAS *N*O*R*T*O*N* Verslo planas Mokslinis vadovas: dėst.Lukoševičius Autorius: VV-2/2 gr. stud. Andrius Gudleikis KAUNAS, 2004 TURINYS Įžanga ............................................................................................ 1. Verslo tikslai ............................................................................... 1.1 Verslo plano paskirtis ....................................................... 1.2 Bendrieji verslo uždaviniai ................................................ 1.3 Specijiniai verslo tikslai ..................................................... 2. Tikslų medis ................................................................................ 3. Svarbiausios žinios ..................................................................... 3.1 Ekonominiai veiksniai ...................................................... 3.2 Socialiniai veiksniai ........................................................... 3.3 Politiniai veiksniai ............................................................ 3.4 Šakos analizė. Rinkos dydis .............................................. 4. Personalas .................................................................................. 5. Vadovavimas .............................................................................. 6. Paslaugos ir kliientai...
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...Resume of Ridwan Ferdous House No: 148/1, Road No: 2, Block: A, Section: 12, Dhaka: 1221 Cell: +8801926430179 E-mail: bahar007@ymail.com Career Objective: To obtain a part time job in an established and well-structured business organization, where from I can gather modern and scientific knowledge about business and communication skills and will be able to develop analytical powers and utilize my abilities. Academic Qualification: Bachelor of Business Administration (BBA) Year of Passing: 2007-Present Institution: Southeast University C.G.P.A: 3.25 (in the scale of 4.00) Higher Secondary School Certificate (Commerce) Year of Passing: 2006 InstitutionK. K. Govt. Institution, Munshigonj G.P.A: 4.50 (in the scale of 5.00) Secondary School Certificate (Science) Year of Passing: 2004 Institution: Narayngonj University College, Narayngonj G.P.A: 4.06 (in the scale of 5.00) Additional Information: ▪ I have the power to convince people. ▪ I have the power of Creativity, Self-driven and Leadership quality. ▪ I have the ability to work hard and willingness to learn from the seniors. ▪ I also perform my work very well with my colleagues. Area of Interest: Marketing, Human Resource Management & Brand Management. Computer literacy: ▪ Expert in Microsoft Word, Microsoft Power Point, Microsoft Excel ▪ Operating System: Windows 98/XP/Vista. ▪ Excellent knowledge on Internet and its basics. Language...
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...The Mongols did more cultural destruction. And there don't put some changes in culture.The Mongols were barbarians since in their battalions if a group of 3 or more soldiers fled from battle they were executed. Furthermore, if 10 escaped, the rest of the battalion were executed. Furthermore, a technique that they had was to place their children and wives at their disposal so that the enemy would think that there were many people. In addition to this, they make captives and people from other parts to fight in front of wars. Also another thing they do is make fat from people who have died and throw it at them while they throw fire that is almost inextinguishable. Document 2 and 3.The conquest of Nishapur The Mongols in the spring of 618/1221...
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...Binder and A. Milchev, J. Polym. Sci. B 50, 1515 (2012). 16M. Tagliazucchi and I. Szleifer, Soft Matter 8, 7292 (2012). 17A. N. Constable and W. J. Brittain, Colloids Surf. A 380, 128 (2011). 18N. Li, C. Zuo, and Q. Cao,J. Macromol. Sci. B 51, 275 (2012). 19R. P. Castro, H. G. Monbouquette, and Y. Cohen, J. Membr. Sci. 179, 207 (2000). 20L. Lanotte, S. Guido, C. Misbah, P. Peyla, and L. Bureau, Langmuir 28, 13758 (2012). 21E. M. Sevick, Macromolecules 29, 6952 (1996). 22S. P. Adiga and D. W. Brenner, Nano Lett. 5, 2509 (2005). 23T. Suo, T. N. Shendruk, O. A. Hickey, G. W. Slater, and M. D. Whitmore, Macromolecules 46, 1221 (2013). 24T. Suo and M. Whitmore, J. Chem. Phys. 140, 114901 (2014). 25G. S. Grest and M. Murat, Macromolecules 26, 3108 (1993). 26T. Suo and M. D. Whitmore, J. Chem. Phys. 138, 164907 (2013). 27G. H. Fredrickson, The Equilibrium Theory of Inhomogeneous Polymers (Oxford University Press, New York, 2006). 28R.G.Larson,ConstitutiveEquationsforPolymerMeltsandSolutions(Butterworth Publishers, USA,...
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...During the 13th century a small tribe known as the “barbarians” swept across Asia, Middle East, and Eastern Europe. They were known to be the negative and evil people throughout their civilizations. These people had little thoughts about farming due to them being nomadic and had always eaten meat. They were also known to be very destructive in their path. First off, Genghis Khan’s had violent ideas as one of them being, slaughtering the streets of Chinese capital with human flesh everywhere. A little history about Genghis Khan is that he never had the easiest childhood. He spent most of his teenage years fighting clan rivals since his father was poisoned by a rival. Genghis had won the leadership in 1206 while aspirations began to grow...
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...In the case, the married couple Ted and Ruth are planning to sell their principal residence in Richmond and purchase a new one after that. Meanwhile, they and their families use their vacation home in Maryland on a frequent basis. The main tax issues we need to examine here are (1) How much gain should Ted and Ruth realize and recognize on sale of their principle residence? What is the nature of such gain? Should they include the gain in their gross income? (2) Are Ted and Ruth allowed to deduct expenses for their vacation home on the joint tax return? According to Internal Revenue Code section 121 (a) & (b), individuals can exclude up to $250,000 gain from gross income on sale of a main home (or $500,000 for joint returns) as long as they have owned the home and used the home for a minimum of two years. The two years are aggregating which means your living period in the house does not need to be consecutive. However, the $500,000 limitation does not apply to the married couple in our case since Ted fails the use requirements mentioned above (section 121 (b) (2) (A) (ii)). Since Ted moved into the Richmond home on December 13 of 2010 and that the sale date is September 1 of 2012, his stay in the house is less than two years. The amount they can exclude should be the sum of each spouse’s limitation amount determined on a separate basis as if they had not been married (section 121 (b) (2) (B)). Since Ted would not have owned the house if he had not married to Ruth, the amount of...
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