...the components of the two solutions with Iodine test and ... Discussion: ... Why You Add Hydrochloric Acid in Hydrolysis of Starch. Ingestion of saliva during carbohydrate feeding by ... - SciELO www.scielo.br/scielo.php?pid=S0074-02762006000100016&script... by RR Cavalcante - 2006 - Cited by 5 - Related articles Saliva ingestion by phlebotomine during the carbohydrate ingestion phase is ... The presence of saliva in each type of solution or substrate offered, as well as ... 0.2 ml of apyrase assay buffer pH 8 (50 mM Tris/HCl buffer containing 1 mM CaCl2, ... Saliva ingestion occurred under each condition investigated, as indicated by ... Experiment: investigation of action of saliva and hydrochloric ... https://answers.yahoo.com/question/index?qid... Jun 25, 2012 - 1) Name of enzyme involved 2)specific action(s) of enzymes involved. Two hydrolytic enzymes and an epistemological–historical ... www.scienceinschool.org/2007/issue4/enzymes Science in School Sep 3, 2007 - 5 M sodium hydroxide (NaOH); 5 M hydrochloric acid (HCl); Saliva. ... To demonstrate the test methods, test all four carbohydrate solutions ... Discussion ... more concentrated than is necessary for the activity and that using a ... The authors have recommended the Fehling’s test for this investigation. What is the objectives of investigation of action of saliva and ... www.answers.com › Wiki Answers › Categories › Science › Biology ... of investigation of action of saliva and diluted hydrochloric...
Words: 799 - Pages: 4
...You must use this link to submit the assignment on Practical 3 (Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions.). Please ensure the correct file(s) is (are) uploaded before clicking 'Finish'. Marks will be deducted if instructions in the lab manual and below are not adhered to. By submitting via this link, you acknowledge that you will adhere to all the instructions herein. * Reports are to be submitted in pairs (2 persons per group, both have to submit the SAME report). * Font: Arial, Size: 11, single-spaced. Step 1: Create a WORD file as, ‘Name_Partner's name_Practical group_No.of practical_Title.doc' e.g: Shalini_Prem_PG1_P3_Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions. The following MUST appear on the first page of Word: • Name: • Partner’s name: • Class: P.... • Date of lab class: (Day), (date)..... • Program: Foundation in Science • Unit code: FHSB 1214 • Unit description: Biology I • Year and trimester of study: 2013, trimester 1 • Title of lab report: Practical 3: Investigation of Action of Saliva and 3M HCl in Two Carbohydrates Solutions. • Lecturer’s name: Step 2: On the 2nd page of your Word file present your tables. Report must include: (1) title (2) objective - NO need to write the procedure and materials. (3) Result - Table 1 & 2, based on ACTUAL observation obtained in class. You may read manual page 7, 8 & 27 on how to write the observation. No need to include...
Words: 337 - Pages: 2
...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solutions ____________________________________________________________________________________ Objective: Students are expected to state the objective of this experiment. Apparatus & Equipments: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath, ~ 37 oC Water bath, ~ 95oC Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3 M Hydrochloric acid 3 M Sodium hydroxide Procedures: 1. Prepare two boiling tubes containing 1 ml solution A and 1 ml solution B respectively. Add 1 ml Benedict’s solution to each tube. Heat both tubes together in the (~95oC) water bath for two minutes. Record the results in table 1. 2. Add a few drops of fresh solution A and B separately spaced on a white tile. On each solution, add 1-2 drops of iodine solution. Mix with pen cover. Record your observations in the table 1. 3. Pipette 2 ml solution B into each of four boiling tubes. Label the tubes 1, 2, 3 and 4 respectively near mouth of tube. Label your group name. 4. Place tubes 1 and 2 in a water bath of ~37oC. (It doesn’t matter how long you put it in at this stage as no saliva or HCl have been added yet). 5. Salivate into a small beaker till it reaches about 5 ml. 6. Step (6) and (7) is to be done approximately at the same time. Measure out 4 ml of the saliva prepared in step (4) and pipette 2 ml each into tubes...
Words: 898 - Pages: 4
...Topic: Investigation of Action of Saliva and 3 M Hydrochloric Acid In Two Carbohydrate Solution Objective: To investigate the action of saliva and 3 M Hydrochloric Acide in two Carbohydrate Solution Apparatus & Equipment: Boiling tubes Beaker Graduated plastic dropper Water bath, (~ 37oC) Water bath, (~ 95oC) Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3 M Hydrochloric acid 3 M Sodium hydroxide Iodine solution Procedures: Part 1 1. Two boiling tubes were prepared containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube. Both tubes were heated together in the (~95oC) water bath for two minutes. The results were recorded in table 1. 2. Few drops of solution A and B were added separately on a white tile. On each solution, 1-2 drops of iodine solution were added. The observations were recorded in the table 1. Part 2 3. The boiling tubes were labelled 1, 2, 3 and 4. 2 ml of solution B were pipette into each of four boiling tubes. 4. Tubes 1 and 2were placed in a water bath of ~37oC to heat up the solution. 5. The saliva was salivated into a small beaker until it reaches approximate 5 ml. 6. This step was done approximately at the same time. 2 ml of saliva prepared were pipette into tubes 1 and 4. The contents of the tubes were shaken well to ensure thorough mixing. 2 ml of HCl were pipette into tubes 2 and 3. 7. Tubes 1, 2, 3 & 4 were incubated at their...
Words: 550 - Pages: 3
...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath,~37°C Water bath,~95°C Stop watch Test tube holder Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3M Hydrochloric acid 3M Sodium hydroxide Procedures: 1. Prepared two boiling tubes with containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube and heated both tubes together in the (~95°C) water bath for two minutes. Then, recorded the results in table 1. 2. Added a few drops of fresh solution A and B separately spaced on a white tile. On each solution, added 1-2 drops of iodine solution and mixed with pen cover. Recorded your observations in the table 1. 3. Pipetted 2 ml solution B into each of four boiling tubes. The tubes were labelled 1, 2, 3 and 4 respectively near mouth of tube. Labelled your group name. 4. Placed tubes 1 and 2 in a water bath of ~37°C. 5. Salivated into a small beaker until it reached about 5 ml. 6. At the same time, step (6) and (7) was to be done approximately. Measured out 4 ml of the saliva prepared in step (4) and pipetted 2 ml each into tubes 1 and 4. The contents of the tubes shook well to ensure...
Words: 2429 - Pages: 10
...LAB REPORT | Practical 3: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions | LECTURER’S NAME | Ms. Bong Siew Mee | Title: Investigation of Action of Saliva and 3M Hydrochloric Acid in Two Carbohydrate Solutions Objective: To investigate the action of saliva and 3M hydrochloric acid in two carbohydrate solutions. Results: Table 1: Observations made when two carbohydrate solutions provided in laboratory were tested with Benedict and Iodine solution. | Observations | Conclusions | Solution A | Benedict’s test: An initial blue translucent mixture turned to brick-red opaque solution and moderate amount of precipitate settled after heated at a high temperature for two minute. | Presence of reducing sugar | | Iodine test: The translucent colouration of the mixture retained its yellowish-brown colour. | Absence of starch | Solution B | Benedict’s test: The translucent colouration of the mixture remained its blue colour. | Absence of reducing sugar | | Iodine test: The initial yellowish-brown translucent mixture turned to bluish-black opaque solution when solution was mixed. | Presence of starch | Table 2: Colour reactions of Benedict’s test for saliva and 3M hydrochloric acid in two carbohydrate solutions provided in laboratory. Tube | Contents...
Words: 992 - Pages: 4
...Lee Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into tubes 2 and 3 . 7. Tubes 1,2,3,4 were incubated at their respective temperatures...
Words: 892 - Pages: 4
...Jer Win Chong Hui Li Title : Investigation of Action of Saliva and 3 M Hydrochloric Acid in Two Carbohydrate Solutions . Objective : To Investigate of Effect of Salive and Hydrochloric Acid in Carbohydrate Solutions . Apparatus & Equipment : Boiling tubes , Beaker , Graduated plastic dropper , Water bath, ~ 37 oC , Water bath, ~ 95oC Materials : Carbohydrate solution A , Carbohydrate solution B , Benedict‟s solution , 3 M Hydrochloric acid , 3 M Sodium hydroxide , Iodine solution Procedures : Part 1 1. Two boiling tubes containing 1 ml solution A and 1 ml of solution B were prepared respectively . 1ml of Benedict solution was added into each tube . Both tubes were heated together in the 95°C water bath for two minutes . Results were recorded in table 1 . 2. A few drops of solution A and B were added separately on a white tile . 1-2 drops of iodine solution were added on each solution . Part 2 3. Boiling tubes were labeled 1,2,3,4 . 2 ml of solution B was pipette into each of four boiling tubes . 4. Tubes 1 and 2 were placed in a water bath of 37°C to heat up the solution . 5. Small beaker was salivated till it reached about 5 ml . 6. This step was done approximately at the same time . 2ml of saliva prepared was pipetted into tubes 1 and 4 . The contents of tubes were shaken well to ensure through mixing . 2ml of HCL was pipetted into tubes 2 and 3 . 7. Tubes 1,2,3,4 were incubated at their respective...
Words: 892 - Pages: 4
...Practical 3 Investigation of Action of Saliva and Hydrochloric Acid in Two Carbohydrate Solution | Objective: 1. To show the action of saliva in two carbohydrate solutions. 2. To show the action of hydrochloric acid in two carbohydrate solutions. Apparatus & Equipment’s: Boiling tubes Metal test tube racks Beaker Graduated plastic dropper Water bath,~37°C Water bath,~95°C Stop watch Test tube holder Materials: Carbohydrate solution A Carbohydrate solution B Benedict’s solution 3M Hydrochloric acid 3M Sodium hydroxide Procedures: 1. Prepared two boiling tubes with containing 1 ml solution A and 1 ml solution B respectively. 1 ml Benedict’s solution was added to each tube and heated both tubes together in the (~95°C) water bath for two minutes. Then, recorded the results in table 1. 2. Added a few drops of fresh solution A and B separately spaced on a white tile. On each solution, added 1-2 drops of iodine solution and mixed with pen cover. Recorded your observations in the table 1. 3. Pipetted 2 ml solution B into each of four boiling tubes. The tubes were labelled 1, 2, 3 and 4 respectively near mouth of tube. Labelled your group name. 4. Placed tubes 1 and 2 in a water bath of ~37°C. 5. Salivated into a small beaker until it reached about 5 ml. 6. At the same time, step (6) and (7) was to be done approximately. Measured out 4 ml of the saliva prepared in step (4) and pipetted 2 ml each into tubes 1 and 4. The contents of the tubes shook well to ensure...
Words: 321 - Pages: 2
...Identification of Unknown Carbohydrate Solution and Investigation of Action of Saliva and Hydrochloric Acid in Carbohydrate Solution at Two Different Temperatures Objective To investigate the action of saliva and 3 hydrochloric acid in two carbohydrate solution Apparatus Boiling tubes, Metal test tube racks, Water bath, 37-40 Degree Celsius, Water bath, 90-95 Degree Celsius, Beaker, Dropper, Wooden Holder Materials Carbohydrate solution A , Carbohydrate solution B , Benedict’s solution , 3 M Hydrochloric acid, 3 M Sodium Hydroxide Procedures 1. Two boiling tubes are filled with 1 ml solution A and 1 ml solution B respectively. 1 ml of Benedict’s solution is added into each test tube. Both tubes are heated together in the hotter (90-95°C) water bath in two minutes. The results are recorded in Table 1. 2. A few drops of fresh solution A and B are spaced separately on a white tile. I2/KI solution (iodine) is added 1-2 drops on each solution. The solution is mixed with a glass rod on the tile. The observations are recorded in the Table 1. 3. 2ml solution B is added into each of four test tubes and the tubes 1, 2, 3, and 4 is labelled respectively with labelling paper near mouth of tube. 4. Tubes 1 and 2 is placed in a water bath of 37°C. 5. A measuring cylinder is salivated till it reached 5ml. 6. 2ml of saliva is added into tubes 1 and 4. The contents of the tubes are shaked well to ensure thorough mixing. 7. 4ml of HCL is measured and 2ml...
Words: 481 - Pages: 2
...Catabolism: Larger molecules are broken into smaller molecules (mouth, stomach, duodenum). In the GI tract, this is called digestion and can occur by either mechanical or chemical means. Anabolism: Smaller molecules are used as building blocks for larger molecules (liver). The Digestive System Mechanical digestion includes all movements that facilitate catabolic processes: Mastication Swallowing Mixing Increase contact of food with digestive chemicals Peristalsis Movement of muscles within the GI tract that facilitates movement of food The Digestive System Chemical digestion is mainly accomplished by using water (and enzymes) to break chemical bonds (hydrolysis). Fats are broken down into fatty acids and glycerol. Carbohydrates are broken down from polysaccharides into monosaccharides. Proteins are broken down into polypeptides and amino acids. Layers of the GI Tract The wall of the GI tract from the lower esophagus to the...
Words: 3786 - Pages: 16
...Ions 4. ACTIVITY 1A /1B Subcellular components of the Living Cell• Qualitative Tests – Carbohydrates • Molisch Test • Benedict’s Test – Proteins • Biuret Test • Xanthoproteic Test – Lipids • Sudan Test • Acrolein Test 5. ACTIVITY 1A /1B Subcellular Components of the Living Cell• Carbohydrates – Molisch Test 5 drops of the supernate + Molisch Reagent shake Layer with 1 mL conc. H2SO4Control: 1% LecithinMolisch Reagent: α-Naphthol dissolved in Ethanol 6. ACTIVITY 1A Subcellular components of the Living Cell• Carbohydrates – Molisch Test 5 drops of the supernate + Molisch Reagent shake Layer with 1 mL conc. H2SO4Principle: when sugar solution is mixed with alpha- naphthol is brought in contact with conc. H2SO4, a violet ring is formed at the junction of the 2 liquids 7. ACTIVITY 1A Subcellular components of the Living Cell• Carbohydrates – Molisch Test 5 drops of the supernate + Molisch Reagent shake Layer with 1 mL conc. H2SO4H2SO4 : acts as dehydrating agent forming furfural derivatives which interact with alpha-naphthol liberating a colored compound 8. ACTIVITY 1A /1B Subcellular Components of the Living Cell• Carbohydrates – Benedict’s Test 1 mL of Benedict’s Reagent + 2 drops of supernate Boil for 2 minutes and allow to coolBenedict’s Reagent: Sodium Citrate, Sodium Carbonate, Sodium Hydroxide, and Copper Sulfate 9. ACTIVITY 1A/1B Subcellular components of the Living Cell• Carbohydrates – Benedict’s Test• used to detect the presence...
Words: 2072 - Pages: 9
...Human Physiology (HP1) Practical Report Experiment on Salivary Secretion and Taste Khoo Wei Cheng Kevin BBSD1 1013A 5/5/2011 Introduction Saliva secreted by the salivary glands in our mouth serves to lubricate the mouth to facilitate speech as well as to moisten food and aids in compacting it into a bolus for digestion. It is an important component in the maintenance of our dental hygiene as it cleanses the mouth. It contains enzymes that can chemically break down starchy food. Saliva also dissolves food chemicals so that they can be tasted and render gustatory satisfaction to the diner. It is secreted by 3 pairs of salivary glands: the parotids, submandibulars and sublinguals. Salivation from these glands is controlled primarily by the parasympathetic nervous system. When food is ingested, salivary nuclei in the brain stem (pons and medulla) receive signals sent from chemoreceptors and mechanoreceptors in the mouth. As a result, facial (VII) and cranial (IX) nerves are innervated and trigger secretion of saliva. Rate of secretion and the composition of saliva depend on type of stimulus used. Taste plays a vital sense that is important from an evolutionary perspective. There are basically four types of taste sensations: sweet, salty, sour and bitter. Sweet taste is produced by organic substances such as sugars, saccharin and some amino acids. Sour taste is elicited by acids whereas salty taste is produced by metal ions (inorganic salts). The bitter taste is elicited...
Words: 1630 - Pages: 7
...Carnivores (other animals) vs. Omnivores (All) * Most animals are opportunistic feeders – eat outside their standard diet when their usual foods aren’t available * Animals must eat * But, to survive and reproduce balance their consumption, storage, and use of food 33.1: AN ANIMAL’S DIET MUST SUPPLY CHEMICAL ENERGY, ORGANIC MOLECULES, AND ESSENIAL NUTRIENTS * Diet must satisfy 3 nutritional needs: * Chemical energy for cellular processes * Organic building blocks for macromolecules * Essential nutrients * Activities of animals depends on sources of chemical energy that is used to produce ATP * To meet the continuous requirement for ATP, animals ingest and digest nutrients * Carbohydrates, proteins, lipids for cellular respiration and energy storage Essential Nutrients * Essential nutrients are preassembled organic molecules and minerals * Obtained from an animal’s diet * Include essential fatty acids and amino acids, vitamins, minerals * Key function: serve as substrates, coenzymes, and cofactors in biosynthetic reactions * Essential Fatty Acids and Amino Acids * Animals convert fatty acids to a variety of cellular components * Membrane phospholipids, signaling molecules, storage fats * Essential fatty acids – animals can’t synthesize (but plants can) * Contain one or more double bonds * Example: linoleic acids, seeds, grains * Essential...
Words: 6577 - Pages: 27
...Table of Contents: FHSB 1214 FHSC 1214 Biology I Cell Biology Introduction Practical 1 Practical 1 Cell Biology Biological Studies I molecules I Practical 2 Practical 2 Cell Biology Biological Studies II molecules II Practical 3 Cell Biology Studies III Practical 4 Cell Biology Studies IV Practical 8 Cell Biology Studies VIII Practical 5 Cell Biology Studies V Practical 6 Cell Biology Studies VI Practical 7 Cell Biology Studies VII Practical 9 Cell Biology Studies IX Practical 10 Cell Biology Studies X - Experiment Description Page Writing of Lab Reports Identification of Biomolecules 5 13 Identification of Unknown Carbohydrate Solutions and Investigation of Action of Saliva and HCl in Carbohydrate Solution at Two Different Temperatures Investigation of the Effects of Catalase Concentration on Hydrogen Peroxide Decomposition 20 Synthesis of Starch Using an Enzyme Extracted from Potato Tuber Investigation of the Effects of Different Catalytic Conditions on Hydrogen Peroxide Decomposition Microscopy 27 Practical 6 Cell studies II Practical 7 Cell studies III Extraction of Cell Organelles by Cell Fractionation Determination of Solute Potential of Potato Cell Sap 47 Practical 8 Cell studies IV Effects of Different Treatments on Stained Potato Cells 64 Practical 9 Energetics I Respiration of Germinating Beans 67 Microscopic Examination of Cells at Various Stages...
Words: 22060 - Pages: 89
