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Antimicrocerum Lab Report

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3.4 Antimicrobial activity of compound methanol extract against Ralstonia solanacearum and Fusarium oxysporum
Strain FJAT- 42192 compounds methanol extract was tested against Ralstonia solanacearum FJAT-91 and two Fusarium oxysporum formae species: F.oxysporumf. sp. momordicae nov. f. FJAT-3015 and F.oxysporum f. sp. Melonis FJAT- 9230. The strain methanol extract exhibited antimicrobial activity against all pathogen used Fig 3.7. Interestingly, against Ralstonia solanacerum the strain exhibited a very high antagonist activity therefore Ralstonia is the most sensitive pathogen. Morever against both Fusarium oxysporum pathogen, strain was able to reduce the mycelium growth compare to the control plate. Althought the reduction was not very high. According to this results the compound methanol extract from Bacillus strain FJAT- 42192 contain indeed antifungal and antibacterial peptides compounds. …show more content…
图 3.7 菌株FJAT- 42192抗菌物质对青枯菌和镰刀菌的拮抗活性 (A2)
Notes: (A2): Ralstonia solanacearum, (B2) Fusarium oxysporum f. sp. Melonis FJAT- 9230 (C2) Fusarium oxysporum f. sp. momordicae nov. f. FJAT-3015. (A1), (B1) and (C1) represent the control plates
3.5 Effect of volume on compounds methanol extracted antagonist activity against Ralstonia solanacearum FJAT-91
Different volume of the dissolve compounds were used to determine the low volume required for the compounds activity .Volume used were: 10µl, 20 µl, 40 µl, 60 µl, 80 µl, 100 µl. The highest antimicrobial activity was registered when the highest volume (100µl) with 20mm of inhibition zone. No inhibition was observed when 10 µl of methanol extract was used. Results presented here shows that the antimicrobial activity of Bacillus methylotrophicus FJAT- 42192 depend on the volume of the compounds extract. Results are presented in Table 3.5 and Fig

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