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Biology Internal Assessment

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Colorimetry / Beer's Law Laboratory

Each group will make 5 dilutions of a copper sulfate solution. As a group you will decide on appropriate dilutions and how you will go about doing it. You should start by making 0.10 M solution from solid copper (II) sulfate pentahydrate. As a group you will decide how much to make.

Remember that for a trend you need to have 5 data points and ideally your the range on the y-axis will be about an order of magnitude or more.

Use appropriate equipment: volumetric flask (the smallest I have is 50 mL), graduated pipets, burets, volumetric pipets. Be careful to distinguish between equipment that is meant “To Deliver” and that meant “To Contain”

Take absorbance readings of your copper sulfate solutions then make an unknown solution by mixing some measured amounts of a couple of your dilutions to make an unknown for another group. Measure the absorbance and calculate its concentration using your calibration curve AND by calculating based on the volumes you mixed.

Don't forget to use a blank! Don't forget to handle the cuvet properly and wipe sides with kimwipes

Obtain an unknown solution from another group (one they have especially made for you!) and test the absorbance. Determine the concentration using the graph AND using the line of best fit. Compare to the other group's result. (Compare means calculate percent error and discuss differences.)

Comment on the linearity of your calibration curve.
Identify random and systematic errors present in this lab

Grading: 1. Data notebook
**must include some procedural information so I know what equipment you used and how you made dilutions 2. Data collection 3. Data processing 4. Error Propagation 5. Conclusion: Summarize the best practices for using this technique for determining an unknown concentration. (In

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