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Blowfly

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container). There is one spray top per group (use it with the boiling tubes). Start with the lowest concentration of pesticide and work upwards. The spraying of pesticide must be carried out in the fume hoods. Briefly spray the pot through the mesh netting so that the pesticide comes into contact with the flies. Before spraying make sure none of the flies are on the netting, as otherwise they will get a larger dose than those on the bottom of the container. You should also use the same procedure for each pot. Note that since there are only 11 pots, 1 pot will be the control and 5 pots will be used for each pesticide. Ensure that you use the following concentrations of rotenone (0.03, 0.1, 0.3, 1 and 3x for Rotenone) and primicarb (3 x 10-8, 3 x 10-7, 3 x 10-6, 3 x 10-5 and 3 x 10-4M for pirimicarb).

It is important that you start with the control, then the most dilute solution of one of the pesticides. Work your way up to the top concentration of the pesticide, then thoroughly wash the spray top with distilled water. Then you should use the spray top to dispense control solution in order to clean the inside of the spray. Now you can move onto the most dilute solution of the 2nd pesticide and work your way up to the top concentration.

When dispensing pesticide solutions it is essential that you use a new clean pipette tip for each solution to avoid cross contamination of solutions.

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