Ensure that all experimenters are wearing goggles.
Acquire 5 test tubes, a test tube stopper per test tube, and one test tube rack. Place all five of the test tubes in the test tube rack.
Cut out circles from the 2 filter papers using a 6mm hole puncher.
Obtain a 500mL bottle of 30% hydrogen peroxide, and measure 25.0 mL [±0.5mL] of Bos Taurus Liver Catalase Enzyme Solution (4.0 mol/L), with the use of a 50.0 mL graduated cylinder.
Pour the 25.0 mL enzyme solution into a 250 mL beaker.
Using the (cleaned) 50.0 mL graduated cylinder, measure 10.0 mL [±0.5mL] of hydrogen peroxide. Pour 10.0 mL of H2O2 into one of the 5 test tubes, with the use of a funnel.
To control the temperature of the hydrogen peroxide, pour 300 mL (as measured by a 1000 …show more content…
Place the thermometer into the hydrogen peroxide and wait for the water to reach a temperature of 37.0 ⁰C. Move the temperature dial above and below this temperature for the duration of the trials to ensure the temperature of the substrate is maintained.
Immerse a filter disc into the Catalase enzyme solution in a beaker with the use of tweezers. Any solution that is not absorbed by the disc can be removed by dabbing it on a paper …show more content…
Repeat steps 6-13 for trials 2-5, recording the time (quantitative [±0.5s]) and qualitative observations of the solutions prior, the reaction, and the final solution.
Acquire 5 test tubes, a test tube stopper per test tube, and one test tube rack. Place all five of the test tubes in the test tube rack.
For Condition 2 substrate concentration, using the 50.0 mL graduated cylinder, measure 8.0 mL [±0.5mL] of hydrogen peroxide. Pour the 8.0 mL of H2O2 into one of the 5 test tubes, using a funnel. Rinse the graduated cylinder and funnel and dry, after use.
Measure 2.0 mL of distilled water using the cleaned 50.0 mL graduated cylinder. With a funnel, pour this into the same test tube, with the 8.0 mL of H2O2.
Using the same 300 mL water from the previous condition, heat the water to 37.0 ⁰C.
Repeat steps 8-13 to complete the rest of the trial. Repeat steps 15-18 for trials 2-5, recording the time (quantitative [±0.5s]) and qualitative observations of the solutions prior, the reaction, and the final