The purpose of this experiment was to identify which DNA mutation is responsible for the brown eye phenotype in sepia flies. To answer our research question, multiple mini-experiments were successfully conducted. In the beginning we extracted the DNA from both, wild and mutant flies. Then, we ran an electrophoresis image, Figure 1, to ensure that the DNA was extracted. From this gel image there was no discrete difference between the wild and mutant flies; furthermore, that is because the DNA region of interest was too large to be viewed all at once. Analyizing G3, the lanes that lacked RNase had a bright smear, while the lane that contained RNase didn’t. This is because RNA interferes with the gel image (CMMB, 2018). In the next step, the DNA region of interest was amplified using two distinct primer sequences, since it was too large to be view all at once. Then, we ran an electrophoresis image, as shown in Figure 2. From this image, we concluded that using primer set one (G1), the DNA sequence length of both subjects were equivalent; thus, if there were a mutation it must not be a deletion. Using primer set 2 (G2), the DNA sequence of the mutant flies was deficient in approximately …show more content…
There were two samples for each phenotype in each primer set. To ensure we chose the most suitable sample, a Nanodrop Lite was utilized. This piece of technology provided the purity and concentration of each sample as seen in Table 1. The desired purity is 1.8 and the higher the concentration the more favorable. The most suitable sample for both the wild and mutant flies was sample A. This decision was determined by considering the purity first and then the concentration. Although the mutant B sample had a higher concentration than sample A, however, the purity was lower. The higher the concentration, the less amount of plasmid DNA needed for the restriction digest and sequencing experiments (CMMB,