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...4110065A.qxp 9/25/2007 2:39 PM Page 1 ™ Quick Start Bradford Protein Assay Instruction Manual For technical service call your local Bio-Rad office, or in the US, 1-800-4BIORAD (1-800-424-6723) 4110065A.qxp 9/25/2007 2:39 PM Page 5 Table of Contents Section 1 Introduction 1 1.1 Principle 1 1.2 Selecting a Protein Standard 5 1.3 Product Description Section 2 Instructions 9 11 2.1 Standard Assay Protocol 11 2.2 Microassay Protocol 14 Section 3 Data Analysis 18 Section 4 FAQs and Troubleshooting 22 Section 5 Ordering Information 26 Section 6 References 28 Section 7 Appendix 30 4110065A.qxp 9/25/2007 2:39 PM Page 7 Section 1 Introduction The Quick Start Bradford protein assay is a simple and accurate procedure for determining the concentration of protein in solution. It provides ready-to-use convenience by supplying the dye reagent at 1x concentration and two protein assay standards at seven prediluted concentrations. The prediluted standards are conveniently packaged in 2 ml screwcap vials, eliminating wasteful and sharp ampoules, and ensuring protein stability over the shelf life of the product. 1.1 Principle The Bradford assay is a protein determination method that involves the binding of Coomassie 1 4110065A.qxp 9/25/2007 2:39 PM Page 8 Brilliant Blue G-250 dye to proteins (Bradford 1976). The dye exists in three...
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...Service Request SR-rm-022, Part 2 Ryan Finch BSA/375 After the previous meeting we discussed several important topics that have required additional research, in order to present a safe way to make transitions and new changes. We have also reviewed some different data flow that will work to improve the proper handling within Riordan Manufacturing to ensure the best relationship between each department. Once the final implementation is installed it will not only improve communication within the company but it will also help save time and money. Security Security should be the highest point of interest or concern of any project. Security is mainly the responsibility of the group operating the system, which would make it the company’s staffs responsibility to install and operate security controls such as firewalls, anti-virus software, and also performing routine updates, and data backup and recovery points. All data needs to be kept secure and confidential at all times. Any company data such as policies, procedures, employee, or customer information needs to be secure and it is Riordan Manufacturing’s responsibility to make sure there systems are capable of doing so. All the information on company computers should be password protected, encrypted, and only give authorized personnel access to such information to ensure data safety. If any information especially “sensitive information” were to get into the wrong hands it could really hurt the business...
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...Sr-rm-022 part 3 Shane Edwards BSA/375 August 31, 2015 Joseph Duer Sr-rm-022 part 3 Implementation Stage The final stage of the SDLC and of this project is the implementation stage, in this stage the system is installed and made operational in the product environment after the user and acceptance testing. This stage can be separated into six phases; coding, testing, installation, documentation, training, and support. Each of these phases in crucial to the success of subsequent phases therefore Riordan must pay special attention to each. * Coding- during the creation of this new system for Riordan all programmers must pay special attention to all desired features and functions so that in latter phases errors can be kept at a minimum. The goal of this phase is to create the actually processes of the new system's design within the software. * Testing- In the testing phase all system components are tested at multiple integration levels of the new system to verify the functionality and completeness of the system. Systems requirements are traced throughout the testing phase to identify variances from specifications and requirements. This phase requires user acceptance testing and is an important factor in ensuring that the system satisfies its intended use and meets user needs. Both Alpha and Beta testing will be conducted to thoroughly vet the new system. * Installation- The installation phase will consist of the old Human Resource system being removed or...
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...University of Phoenix rEsource Syllabus Course Title BSA 375– Fundamentals of Business Systems Development Course Schedule Tuesdays, September 19 and 26, and October 3, 10 and 17, 2006 Mesa Campus Course Materials All materials can be found on resource, which can be accessed through the University of Phoenix eCampus Web site. Instructor’s Name Greg Binder Primary eMail Address gbinder@email.uophx.edu Alternate eMail Address gbinder69@qwest.net greg.binder@phoenix.gov Telephone Number(s) 602-256-3589 (W) 602-942-6448 (H) Availability Mr. Binder can be reached by any means above. In many cases, you will have to leave a message, however, he will return your contact as soon as possible. You may call the work number and the voice mail number at any time. Home calls should be limited to 7 am to 10 p.m. For sending documents by e-mail using the Internet, use the following address: gbinder@email.uophx.edu. University Policies Please review University Policies regarding topics such as student conduct, attendance, and plagiarism in your Program Handbook, which can be accessed through the University of Phoenix eCampus Web site. Modality Procedures It is my intention to follow, fairly carefully, the guidelines expressed in the module as to assignments and administration. Please do not be misled: you are responsible for the material in the assignments. The following specific guidelines are provided: The...
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... The RUP life cycle RUP's life cycle consists of four consecutive phases: inception, elaboration, construction, and transition. In the inception phase, the concept behind the process is reiterated and the boundaries of the project are defined. In the elaboration phase, the problem domain is identified and architecture established. In the construction phase, the software is developed. Finally, in the transition phase, the product is sent to the user domain. Inception Phase The first phase of the process reiterates the concept of what they web design departments want. It defines the boundary or the project. Some of the things that will be determined in this phase include external factors, risk analysis as well as cost and budget. The final product of this phase is that a vision document detailing the project requirements, features and constraints. Phases and iterations are incorporated into the project plan. A forecast of the initial business case, risks, and...
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...RIORDAN MANUFACTURING HR SYSTEM ANALYSIS BSA/375 UNIVERSITY OF PHOENIX ONLINE INTRODUCTION Originating in 1992 Riordan manufacturing is a worldwide leader in manufactured plastics. Founded by Dr. Riordan, a chemistry teacher, they currently operate out of four facilities: Hangzhou, Pontiac, Albany, and San Jose. They are a fortune 1000 Enterprise whose revenue exceeds 1 Billion dollars. Their major clients include: The Department Of Defense, automotive parts manufacturers, aircraft manufacturers, and appliance manufacturers to name a few. The system we will be analyzing is their first Human Resources Information System (HRIS), which was implemented in 1992. It is integrated into the company's financial system, use for keeping record of employee's data. That data includes: - Personal Information - Hire date - Pay Rate - Vacation Hours - Tax information - Date of Seniority - Organizational Information They plan on updating the current system with a newer, more efficient HR system. The HR integration project has already been approved, and they are awaiting a detailed project plan. this plan is to include the following: budget, tasks, resources, and schedule. There has been a six month deadline set in place for project completion. This will allow the company to begin using the new system, no later than the second quarter of the year. KEY STAKEHOLDERS In gathering information for the project, the following stakeholders have been identified as excellent...
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...BSA/375 SR-rm-022 Part 1 Riordan Manufacturing is a fortune 1000 company, who is over seen by Riordan Industries. Riordan Plastic, Inc. was started in 1991, then changed to Riordan Manufacturing in 1993. Riordan employs 550 workers to oversee the company’s production of several plastic products. To supply a wide range of products to their very differently associated cliental. This is done through four prime locations, three of which are located in the US and the other is in China. Continuing with an overview of the Riordan’s works and future interests for the companies’ goals. They are constantly working a top of the line research and development team to adapt and create new solutions for customer’s wants and needs. Aiming to assist and not hinder their clients, hoping for long term relationships that benefit both parties equally. The focus to stay on top of the industry is brought on by keeping knowledgeable employees and great quality products for their partners. This is why a successful Human Resource system is so vital to the companies’ abilities to run optimally. There are a few things to consider when attempting to make changes on a large scale for a company. A request for adapting the existing human resource system and adding the ability for new and more efficient system to run in aid of the company. An initial step is to look at stakeholders and who’s input to take into account for these system changes. Riordan works with a combination of large and small customers...
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...BSA/375 Week 2/3 IA DD7 Instructor DIDIER OPOTOWSKY March 3, 2012 Riordan Manufacturing is a company that specializes in plastic injections for making products. Recently the COO, Hugh McCauley requested a project plan to discuss the steps and roles involved in creating an updated HR website. This document will cover the steps involved in Analyzing the current system as well as what techniques where used to gather the information and the design. We will address the scope of the project and how feasible the implementation of the proposed changes would be to the organization. Riordan Manufacturing is a worldwide company that has its headquarters located in San Jose, California. Riordan has a site located in Hangzhou, China that is responsible for making Plastic Fans. The other two locations are in Pontiac, MI, and Albany, GA. Michigan’s plant handles Custom Plastic Parts, and Georgia’s plant makes plastic beverages. This project will entail upgrading the current system for HR to a more state of the art system but without losing current abilities from the current site. The current HR site is broken down into categories. The existing categories are spread out as far as topics. There are multiple links for employee information, which should reside in a central location. The project timeline is 6 months from start to finish. Obtaining all information on current system standings and processes will be paramount to the success...
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...Riordan Manufacturing SR-rm-022 Kalin Leonard BSA/375 Business Systems Development April 3, 2014 Richard Epperson Principles of System Analysis and Design Riordan Manufacturing produces numerous plastic products, which serve the needs of various companies around the globe. The Albany, Georgia location produces plastic bottles made to hold beverages. The location in Pontiac, Michigan produces various plastic parts that are needed by different companies, while the division in Hangzhou, China produces plastic parts for fans. With their growing business comes a need to implement software that can excel in the fast-paced, technology-driven society we live in. The current human resources information system keeps track of personal information, such as, name, address, marital status, birthday, and more. The system also tracks pay rate, personal exemptions (for tax purposes), hire date, seniority date, organizational information, and vacation hours. Riordan Manufacturing Incorporated’s Chief Operations Officer, Hugh McCauley, has requested the company implement a state-of-the-art information system. This system must integrate all existing human resource tools and various software into one single robust application. The system must also be completed within a timeframe of six months. In order to achieve this goal, the company must conduct an extensive amount of research to ensure the highest possible quality product will be delivered. When conducting this research, various...
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...Service Request SR-rm-022 Part 1 Larry Cohen BSA 375 Jeffery Comrie 10/13/14 Background Information The COO, Hugh McCauley, of Riordan Manufacturing, wants to combine the existing variety of tools in use into a single integrated application. The business wants to take advantage of the more sophisticated state-of-the art information systems technology that already exists in the human resources department (Riordan Manufacturing, 2006). In this document, the stakeholders, the information- gathering techniques, project design plan, business requirements, scope, and feasibility will be discussed. Stakeholders and Key Roles Recognizing the stakeholders and defining their participation within a company is essential to achieving the organizational objectives. The Stakeholders need to collaborate and interact with each other to guarantee the organization’s success. Stakeholders are defined as “influential people who are vitally interested in the actions of the business.” Every stakeholder big or small has an significant role in Riordan’s tactical plan. The stakeholders of importance are Riordan’s President and CEO, The Vice President of Research and Development, The Human Resource Director, and the employees. Without the direction and input of stakeholders, growth would be impossible to achieve. The President and COO will be able to provided additional history on the company. The VP of Research and Development will be able to be able to discuss the evolution of technology...
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...Service Request SR-rm-022 Part 1 BSA/375 Joshua Eyler April 11, 2016 Morton Smith Key Stakeholders To gather information about the project you would need to talk to the individuals that use the current process that is being upgraded. Also talking to the person who is requesting the project be done. The reason to gather information from them is because they use the current system day in and day out and know what works and what doesn’t. These individuals can give better insight on what they would like to see with the new updated system. Also this group of people have better knowledge of the current system then the IT department and anyone else who may be working on the new updated system. Information Gathering Techniques Certain techniques are used when gathering information for a system analysis when updating or replacing an old out of date system. These techniques include interviewing, which I mentioned above, handing out surveys to the users of the system, workshops, prototyping, mind mapping, flip charts, and sometimes something as simple as sticky notes can work. Using one or more of these techniques will help with the analysis of the purposed project and help point the project in the right direction. For this project I would use interviewing as my first level of gathering information. I feel that the best information will come from the users of the old system to help where the system lacks and acknowledge its strong points. I also would use group workshops...
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...IT Solutions: Proposal for New HR System By Thomas E. Foster Jr. University of Phoenix BSA/375 09/02/2013 Proposal for New Human Resource System In our quest to ever better ourselves the IT department, of Riordan Manufacturing, is submitting this Request for Proposal to integrate a more sophisticated, state-of-the art, information systems technology in our Human Resources department. Riordan Manufacturing has always strived to better ourselves by keeping up with technology in all of our operating systems and our manufacturing systems. Our goal is to remain at the forefront of the manufacturing community as we keep leading the way in all of our business systems and, at the same time, keeping the feel of a customer friendly company. We want to make sure that our customers can feel safe in the knowledge that we will do what is necessary to fulfill their business needs. As of now, our Human Resource, (HR), Operating System, (OS), has fallen short of this goal. Please understand that the system that is in place, while it has the ability to complete the task at hand is not sufficient enough for our growing organization. Right now, we are running many separate applications to process all of the HR system requirements. This process is not only time consuming, but is not a cost efficient solution that this company is looking for. If we want to accomplish our goal we need to combine all the old HR systems into a single integrated application. The IT department is...
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