Introduction C.elegans, are soil nematodes that are used as model organisms because they can answer simple questions about the development and behavior in several different organisms based on genetics. It is an excellent model organism for the fact that it is inexpensive, easy to grow on an agar with Escherichia coli, and has 800 cells. Generally the C.elegans are a millimeter in length. Their bodies are long and round

Methods
Before we could cross the nematodes we had to learn how to transfer the worms between agar plates that have E.coli on them. A sterile technique was used which involved closing the lids on the plates when we were not using them. The only object that could touch the agar was our sterilized wire tool. When we handled the worms, the wire pipette was passed through a flame to sterilize it. Once there was glue on our tool from the outside of the bacterial lawn, we could pick up the worm that we desired with the help of our microscope. This was done by scooping underneath and lifting them up or by attaching them to the glue. When the worm was on our tool, they were …show more content…
The first step was to make two parental crosses. The first cross was between five wild type males (+;+) and three L4 dpy-1 unc-32 (dpy; unc) hermaphrodites. The second cross was between five wild type males and three dpy-11 unc-32 hermaphrodites. In each cross, the hermaphrodites were self-fertilizing. We allowed the nematodes to mate and observed the F1 generation. From each cross that we previously produced, we took one wild-type L4 hermaphrodite and transferred it to a new plate. We repeated this process until we had two plates from each cross that had self- fertilized hermaphrodites. These four plates made up the F2 offspring. The F2 progeny on the four plates were observed. Our goal was to count the number of adult Wild-type, Uncoordinated, Dumpy, Uncoordinated and Dumpy