...UV-Visible Spectrophotometer Calibration S.ZALI M.Sc. Dept. of Analytical chemistry Razi vaccine serum research institute S.Zali@rvsri.ir 2008 The primary objective of GLP is to ensure the generation of high quality data. Essential to this is the ability to produce estimates for the measurement uncertainty associated with calibrations. V The spectrophotometer has become the workhorse for high throughput routine testing for a wide variety of analysts in chemical and biochemical analysis laboratories. It is important for accuracy and reproducibility in measurement that this instrument is correctly set up and calibrated. Increasing Wavelength 50,000 Gamma X-ray 12,820 UV 200 4000 NIR 380 780 IR 2500 400 cm-1 FIR Micro Radio 25 000 nm Increasing Energy K-shell electrons Outer-shell electrons Molecular vibrations Molecular Rotation Conventional Spectrophotometer: The factors that must be calibrated: Control of wavelengths Control of absorbance Limit of stray light Resolution Spectral slit width Cell Control of wavelengths: Wavelength standards: The ideal wavelength standard would have very narrow and very well defined peaks. Control of wavelengths: •Solution of holmium(III) in perchloric acid: 240– 640 nm. •Holmium oxide filters: 280–640 nm. •Didymium filters: 400–750 nm. •Using of spectral lines in low-pressure discharge lamps: 656.1 nm produced by...
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...THE POTENTIOMETER: Internal Resistance of a Test Cell OBJECT To calibrate a one meter slide wire potentiometer using a standard cell and then to use this potentiometer to measure the emf of a test cell. The terminal voltage of the same test cell is then measured as different load resistors are connected across the test cell and these data are used to determine the internal resistance of the test cell. THEORY The electromotive force (emf) of a cell is its terminal voltage when no current is flowing through it. The terminal voltage of a cell is the potential difference between its electrodes. A voltmeter cannot be used to measure the emf of a cell because a voltmeter draws some current from the cell. To measure a cell's emf a potentiometer is used since in a potentiometer measurement no current is flowing. It employs a null method of measuring potential difference, so that when a balance is reached and the reading is being taken, no current is drawn from the source to be measured. Figure 1. This is the basic circuit diagram for a potentiometer. Point C is the sliding contact which can be adjusted for zero current deflection through the galvanometer. In this method (refer to Figure 1) a uniform, bare slide wire AB is connected across the power supply. If you were to connect a voltmeter between the + power supply terminal and point A you would measure essentially zero volts. If you were to now connect the voltmeter between the + power supply and point B you would measure...
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...Wilson Electronics Cellular Booster Database Requirements For Cellular Booster O-14828 Version 1.06 Presented to: Wilson Electronics Approval Name Title Signature Date Confidential Wilson Electronics Cellular Booster Database Requirements - v1.06 O-14828 Change History Version # 1.00 1.01 1.02 1.03 Reason Author Date 2014/04/11 2014/04/16 2014/04/21 2014/04/22 Initial Release Condensed parameters Consensus reached on majority of parameters Added specifics about DB connection, changed AGC Test Parameters Moved some configuration items to test limit sections Al Braga Al Braga Al Braga Al Braga Al Braga 1.04 2014/04/30 1.05 1.06 Merged RF Test Sections, added error, clarified Al Braga some parameter types. Removed all Station Name inputs Al Braga 2014/05/14 2014/05/15 Page 2 of 14 Project File: Cellular Booster Database Requirements.docx ©Averna - Confidential Wilson Electronics Cellular Booster Database Requirements - v1.06 O-14828 Table of content Purpose and Scope ....................................................................................................................................... 4 Considerations .............................................................................................................................................. 4 Database Connectivity .................................................................................................................................. 5 Database...
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...RUNNING HEAD: Quality One Call at a Time Quality One Call at a Time May 30, 2006 Quality scores in many call centers are on the decline. The main goal of a call center is to provide excellent customer service while delivering accurate information in a timely manner. “Fast is fine, but accuracy is everything.” Wyatt Earp. Wyatt Earp may not have said this quote in reference to managing quality at call centers, but it carries the same relevance. Management at call centers tends to focus on other issues instead of the accuracy of the information that the CSR is relaying to the customer. Thus value is misplaced and the quality at call centers starts to head on a downward path. One issue that could drive quality scores down is ineffective training. At a call center here in Jacksonville, management decided to pull CSRs out of training after the second day to answer calls because the queue was high. This interrupted the training process and added days to the training schedule. The trainer manager was powerless to prevent this from happening. This happened 6 times over the course of the proposed three-week training calendar. Call center trainers must ensure that their trainees’ learning is not hindered by management’s goal to reduce queue times. The constant yo-yo handling of the CSRs resulted in training not being delivered on all topics, which resulted in low quality scores. This has not been a high topic of focus for most call center management staff. ...
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...Math – Final Exam Vet 110 Name: _______________________ Date: _______________________ Good luck! ( Identify the highest value: 1) 3.7 4.1 2.9 2) 5.4 6.2 4.7 3) 1.2 1.5 1.7 Add the following decimals: 4) 1.35 + 2.42 = ________ 5) 2.8 + 0.03 = ________ Subtract the following decimals: 6) 2.3 – 1.45 = ________ 7) 10.25 – 1.47 = ________ Multiply the following: 8) 1.37 x 0.02 = ________ 9) 0.31 x 0.04 = ________ Eliminate the decimal: 10) 5.25 = ________ 0.3 Solve the following equations; express your answer to the nearest tenth. 11) 12 x 500 x 2000 x 1 = ________ 1 2700 1 60 12) 0.35 x 2.5 = ________ 7. 0.7 13) How many tablets will be needed to give the following dose? The tablets are labeled 1.2 mg. The order is for 0.6 mg. ________ tablet(s) 14) Reduce the following as far as possible: 124 76 = ___________________________ 15) Express the following division to the nearest tenth: 5.7 1.46 = __________________________ 16) Solve the following equation. Express answer to the nearest tenth. 30 x 50 x 10 x 415 1 550 1250 1 = ____________________ Write the following metric measures using their abbreviations and notation rules. 17) Four hundredth of a gram ___________ 18) ...
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...SURFACE WELL TESTING By Karrym Rebay Vol. 1 Preface This is a paper that has been produced to help you better understand and work with the equipment you are familiar with and presently operating on your particular Well Test Unit. It will give you more knowledge about, not only the operation, but also the maintenance and calibration techniques that will help you become more familiar with your own equipment. Paper Content 1. PAPER DESCRIPTION 2. IDENTIFYING A STANDARD WELLTEST PACKAGE AND ITS COMPONENTS 3. EQUIPMENT OPERATION AND FLOWING CONDITIONS 4. SAFETY AROUND YOUR EQUIPMENT 5. CALIBRATION AND MAINTENANCE 6. CALCULATING FLOW RATES 7. GLOSSARY – Exploration & Production Terms 8. CONVERSIONS & TABLES Paper Description This paper describes the specific conditions under which well tests must be performed, lists the surface testing equipment used to perform these well tests, summarizes how this equipment is used to collect samples at the surface and lists several examples that influence the layout of surface equipment. A reservoir test can only be performed under certain conditions. This means the reservoir must be exposed to a disturbance that will cause the reservoir pressure to change. This pressure change, when recorded and interpreted along with the measured flow rates, will give us information about well and reservoir parameters and geometry. A pressure disturbance...
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...We expect that you would be able to find at least 10 to 15 standards for your product. For each standard, you should be able to indicate the answers to each column of the CSR standards inventarisation excel sheet. Namely: Name of the standards you have found relevant to your chosen product Explanation of the purpose of the standard Applicable countries Applicable trade region(s) Application to the chosen product Website address(es) Possible market consequences for the supply chain Example: Name of the standards you have found relevant to your chosen product: Florverde Explanation of the purpose of the standard: Florverde is an initiative of ASOCOFLORES the Colombian association of flowers exporters. The aim of the combined action is to satisfy the requirements of Colombian’s flower 1st importer market the USA. The Florverde certification set up in 1996 enables the thousands of workers involved in the production of export flowers to live and work in acceptable conditions. The certification secures the main USA market and maintain best sustainable practices through annual audits. The main areas for standards include: basic labor regulations, personnel management, work and social welfare, water and irrigation, intergrated pest and disease management, traceability and records. Applicable countries: Colombia Applicable trade region(s): CAN (Andean community) Application to the chosen product: 1. Describe in a much details possible the specifications...
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...| | | | | | | |INSPECTION / |STANDARD (1) |PROCEDURE AND/OR ACCEPTANCE CRITERIA |DOC |SAMPLE |CUSTOMER | |TEST ACTIVITY | | |Y/N(2) |SIZE |INSPECTION | |OPERATION ACTIVITY: 0010 |IN PROCESS | | | |VERIFY PART(S) HAVE RAW MATERIAL TRACEABILITY IS AVAILABLE BEFORE USE | | | | |DRSV010 |CAMERON STANDARD |METHOD- QUALIFIED PERSONNEL TO VERIFY APPLICABLE MATERIAL IS CORRECT BEFORE USE |YES |100% |- | |RAW MATERIAL | |STANDARD- CAMERON STANDARD | | ...
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...| Calibration of a Chromel-Alumel Thermocouple | | Module Title: Engineering ApplicationsModule Tutor: | Student Name: | Submission Date: | | Contents Objectives 3 Introduction 3 Apparatus 3 Theory 3 Results 4 Discussion 4 Conclusion 6 References 6 Calibration of a Chromel-Alumel Thermocouple Objectives * Use a thermometer to tell the changing temperatures of water * To become familiar with the principles of temperature measurement Introduction The purpose of this experiment was to measure the change in voltage in boiling water (electromotive force) against the temperature (measured by a thermometer) when cold water was added at stages. The voltage readings were taken using a Chromel-Alumel thermocouple. 1 6 5 2 Apparatus 1. Immersion heater and power supple 2. 500ml beaker half full with water 3. 3 1000ml beaker full with water 4. Thermometer 5. Thermocouple 6. Digital multimeter 7. Clamp and stand 7 4 Theory Refer to lab sheet for method used. A thermocouple is thermoelectric temperature sensor (which means it can measure temperature using electricity). A thermocouple consists of two wires that are made of different metals to one another which are coupled at the measurement junction and extended to the reference junction. [1] [1] A thermometer is “an instrument for measuring and indicating temperature, typically one consisting of a narrow, hermetically sealed glass tube marked...
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...Method Development For Analysis Of Pesticides In Water Using Chromatography And Mass Spectrometry An Abstract of a Thesis Presented to the Faculty of Chemistry Department Western Illinois University In Partial Fulfillment of the Requirements for the Degree Master of Science By Prashanth Goud Kunuru September 2013 This thesis follows the style and format of the Journal of Analytical Chemistry ABSTRACT Pesticides are chemicals, artificial or natural substances, used for pest control. They have negative influence on human health when accumulated in the body. Numerous studies have clearly linked pesticides exposures to various diseases including cancer and Parkinson’s disease. Water bodies often serve as a key receiver of harmful pollutants emitted from industrial and domestic sources. Pesticides can often enter water through sewage plants, sewer overflows, surface water runoffs, and leaching from agricultural applications or even through improper disposal. Regulatory issues and public concerns over pesticide residues in water supplies have been increasing, and it is important to develop a reliable method for routine test of pesticides in water supplies. Presented here is a novel solid phase extraction technique using disposable pipette extraction (DPX) followed by chromatography and mass spectrometry analysis for determination of pesticides in water. The purpose of the current study was to evaluate the suitability of high pressure...
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...Experimental Physiology (1986) 71, 467-473 Printed in Great Britain FREE INTRACELLULAR MAGNESIUM CONCENTRATION IN FERRET VENTRICULAR MUSCLE MEASURED WITH ION SELECTIVE MICRO-ELECTRODES L. A. BLATTER AND JOHN A. S. McGUIGAN Department of Physiology, University of Berne, Buehlplatz 5, 3012 Berne, Switzerland (RECEIVED FOR PUBLICATION 2 AUGUST 1985) SUMMARY The free Mg concentration ([Mg]i) was measured in ferret ventricular muscle using singlebarrelled Mg-sensitive micro-electrodes. The electrodes were calibrated in solutions containing the [K]i and [Na]i measured in this tissue. In thirteen measurements from seven experiments conforming to strict criteria for calibration and penetration the estimated mean [Mg]i was 04 mmol/l. This is to be regarded as an upper limit since it was estimated by linear extrapolation of the calibration curve. INTRODUCTION Ionized Mg plays an essential role in the regulation of a large number of cellular functions. It is a co-factor in many enzymatic reactions, influences tension development in muscle and modulates amongst others the ionic transport systems in mitochondria (see reviews by Gunther, 1977; Shine, 1979; and Ackerman & Nicholls, 1983). Despite its importance in cellular mechanisms, estimations of the free Mg ([Mg]i) by various methods range from 0- 1 mmol/l to 6 mmol/l (reviews: Flatman, 1984; Gupta, Gupta & Moore, 1984) mainly because of the lack of a simple direct method for its measurement. Direct measurement of [Mg]i is now possible using...
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...supply and multimeter measurements were recorded as meter data for the BI, 5V, and 5I measurements. The EyasSAT GUI was used to measure and record the “raw counts” at the BI, 5V, and 5I locations. The “raw counts” measurements were recorded as counts data. The EyasSAT GUI was used to measure and record the current at the 5I and BI locations as well as measure and record the voltage at the 5V location. The current and voltage measurements made by the EyasSAT GUI were recorded as data collected before calibration. This process was done for five different values of load resistors. The load resistor was removed and the DC power supply voltage was measured and recorded as meter data. The EyasSAT GUI was used to measure and record the “raw counts” for the BV location. The “raw counts” data was recorded as counts data. The EyasSAT GUI was used to measure and record the voltage at the BV location. The voltage measurement made by the EyasSAT GUI was recorded as data collected before calibration. This process was repeated for DC power supply outputs of 10, 9, 8, 7, and 6 volts. A linear regression and coefficient of determination was calculated for the meter data and the counts data for each of the four measurements, BV, BI, 5V and 5I. The counts data was put on the x-axis and the meter data was put on the y-axis. Equations (1) – (3) were used to calculate the linear regressions and coefficients of determination for each data set. Equation (1) shows how the slope of the line (b) was calculated...
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...moment-----------------------------------------------------------------------------2 1.3.2 The Right Angle Assumption--------------------------------------------------------------------------5 2. Apparatus----------------------------------------------------------------------------------------------6 * 3. Data-----------------------------------------------------------------------------------------------------7 * 4. Procedur-----------------------------------------------------------------------------------------------7 * 4.1 Recording-------------------------------------------------------------------------------------------7 * 4.2 Calibration------------------------------------------------------------------------------------------7 5. Results--------------------------------------------------------------------------------------------------8 * 5.1 Determination of the calibration constant----------------------------------------------------8 5.2 Calculation of the Bending Moments----------------------------------------------------------9 5.2.1 Analysis of the oscilloscope traces----------------------------------------------------------9 5.2.2 Calculation of...
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...>>> INSTRUMENTATION KEY FEATURES DESIGN ■ Compact and rugged ■ One button operation ■ Easy to read front mounted backlit display ■ Over-molded boot OPTIONAL SLIP-ON PUMP ■ ■ SENSORS AND CALIBRATION ■ Three sensors. O2, LEL and CO, H2S, or combined CO/H2S sensor ■ User replaceable sensors ■ One button auto calibration ■ Calibration due reminder ■ Sensor span reserve indicators during calibration for predictive maintenance BATTERIES ■ Software controlled pump Powered by the Biosystems MultiPro battery ■ Automatic low flow alarm ■ Automatic leak test before every use RECORDING ■ Automatic event logger (20 events, including sensor type, max, average, time and duration) ■ Black-Box recorder with over 40 hours of storage ■ Available with optional datalogging for convenient and easy downloading to a computer Interchangeable alkaline or rechargeable (Li-Ion) versions ■ Up to 22 hours run time depending on battery type ■ Drop-in charger ■ Recharges in less than 6 hours BIOSYSTEMS MULTIPRO The Biosystems MultiPro is the smallest and easiest to use confined space gas detector offered by Sperian Instrumentation. Extremely affordable, it is packed with features. It offers real time readings of up to 4 gases simultaneously, one-button operation including calibration, an easy to read LCD display, audible, visible and optional vibrating alarms, standard event logger and black box datalogger, an IrDA port for easy communication with a PC, and an optional sample draw...
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...1. Introduction 1 1.1. About ISO 9000 1 1.2. Purpose of internal quality audit 1 1.3. Report structure 1 2. Audit review 2 2.1. Non-compliances 2 2.1.1. Case study 2 – Service and parts processes 2 2.1.2. Case study 3 – QMS manual 3 2.2. Potential for improvement 4 2.2.1. Case study 2 – Service and parts processes 4 2.2.2. Case study 3 – QMS manual 5 2.3. Good practice 5 2.3.1. Case study 2 – Service and parts processes 5 2.3.2. Case study 3 – QMS manual 6 3. Summary 8 4. Appendix 11 Introduction About ISO 9000 ISO 9000 is a family of international standards related to quality management systems (QMS) that facilitates mutual understanding across domestic and international trade. The standards are published by the International Organization for Standardization and available in the UK through the British Standard Institute. ISO 9000:2008 sets down the principles behind the quality management and specifies the QMS terminology, thus forms the basis for the standards within the ISO 9000 family. ISO 9001:2008 specifies the requirements for a QMS an organisation has to fulfil to meet the standard. ISO 9004:2008 provides guidelines for a QMS that aim to improve the performance of the organisation and the satisfaction of both stakeholders and customers. ISO 9000 represents an international consensus on good quality management practices and can be understood as a generic framework to manage a business’ processes to deliver superior quality...
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