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Clinical Benefit of Dark Chocolate

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Clinical benefit and preservation of flavonols in dark chocolate manufacturing
McShea A, Ramiro-Puig E, Munro SB, Casadesus G, Castell M, Smith MA. Clinical benefit and preservation of flavonols in dark chocolate manufacturing. Nutr Rev 2008; 66(11):630-641.

Chocolate is the processed form of cacao beans that undergo fundamental steps of fermentation, roasting, and milling before it is widely available to consumers. This case study examines the chemical composition of chocolate by analyzing the process of dark chocolate production from cacao beans in regards to its antioxidant flavonols content with correlation to positive health benefits.
Fermentation is required to break down the bitter taste of cacao beans in its original form, but lowers the beneficial antioxidant content within the cacao beans by changing the concentrations and composition of procyanidins and flavan-3-ols. After fermentation, the beans are roasted to produce a more favorable taste through Maillard and caramelization reactions of sugars, amino acids, and peptide oligomers. The subsequent step of milling cacao beans into fragments allow flavonoids to interact with other molecules such as theobromine, caffine, and l-leucine which determines both flavor and antioxidant activity in chocolate. Other antioxidant properties have been linked to polyphenols, catechin, and epicatechin in dark chocolate made from cacao beans in addition to flavonoids. Conching is the process after milling, it is a multi-day heat treatment that grinds the chocolate to improve flavor and decrease free acids and volatile by-products. An alternative to conching is the dutching method, it neutralizes acetic acids in cacao. Dutching as opposed to the more gentle conching technique, significantly reduces antioxidant flavonoids content in dark chocolate.
Theobromine and caffeine in dark chocolate are proven to have mild

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