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Cloning
DNA cloning has given major insights into understanding the genetic makeup of an organism. The cloning process will allow the alteration of DNA to better the organism in ways like nutritional value or resistance to diseases. DNA cloning has opened new doors to the studies of genetics. In order to clone DNA, there is a specific method in which selective amplifying is required of DNA sequences, which would in turn generate homogenous DNA populations (Strachan & Read, Chapter Chapter 4, 1999). Cell-based DNA cloning was the first type of DNA cloning to be developed. Foreign DNA fragments in vitro are attached to DNA sequences (Strachan & Read, Chapter Chapter 4, 1999). The new product of DNA is referred too as being recombinant, which just means that the DNA is linked sequences that originate from different sources. Those recombinant DNA fragments are transferred to host cells, which is where they are cloned. These host cells in which recombinant DNA fragments are transferred to must be able to replicate (Strachan & Read, Chapter Chapter 4, 1999). However, in order for DNA to be able to replicate, it must be able to have an origin of replication. In DNA cloning, most is done using modified bacterial or fungal host cells. They are primarily used because they have a higher capacity for rapid cell division (Strachan & Read, Chapter Chapter 4, 1999). The replicons have to go through many cycles of replication even though bacterial cell hosts only have one origin of replication. The two types of extrachromosomal replicons that are created through cell division of a bacterial cell are plasmids and bacteriophages (Strachan & Read, Chapter Chapter 4, 1999). When replication is done, there are many screening processes that can be done to ensure that the cells with recombinant DNA are found. DNA cloning has led to many discoveries in the medical

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