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Dental Stone Nest Case Study

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We placed the ants in 11 subcolonies. 6 subcolonies consisted of 100% majors and 5 subcolonies consisting of 20% majors. 10 workers were in the 100% and 20% groups. The 100% groups contained 10 major workers while the 20% groups contained 8 minor workers and 2 major workers. Also, each subcolony contained 5 larvae in the 2nd instar larvae stage. We placed the ants on ice for a minute until they stopped moving. Then, we added the larvae and the workers to a dental stone nest. This dental stone nest consists of a petri dish filled with dental stone. Also, the dental stone nest had an embedded water reservoir containing a small hole that can be filled with water. For behavioral assays, the petri dish nest was covered with a solid lid to keep

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