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Diagnosis of Infected Person

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The sputum sample of infected patient will identify which bacterium they have contracted. The choices can be numerous, but we’ll look at three types: Bacillus, Escherichia, and Mycoplasma. Bacillus are anatomically rods that produce endospores, and commonly, can be found in soil. A few of its strains are pathogenic to humans, and several used in antibiotics. These several strains are variants of single species; just have different genes carried on the plasmids which can transfer from one bacterium to another. Well known strains are bacillus anthracis and bacillus thuringensis. Bacillus anthracis, or Anthrax, is a disease that affects cattle, sheep, and horses that have adapted/mutated and infect humans. Bacillus thuringensis is a microbial insect pathogen that produce crystalline toxins, and is used to spray on plants to reply and protect from insects. To help identify if this is the causing bacteria, one would use endospore staining, where malachite green is applied to a heat-fixed smear and heated to steaming for 5minutes, and helps penetrates the endospore wall. Then the smear is washed for 30seconds to remove the green from all other cell parts. Next, safranin is applied to the smear to stain the rest of the cell. The smear will be red or pink for cell parts, and malachite green will be the endospores. A gram stain would also will be used to identify the bacterium is bacillus. Bacillus would be a gram-positive, because it retains the violet crystal stain, and appear purple. They are positive because they are easily killed by penicillins and cephalosporins.
Escherichia is the next genera and is the most common inhabitants of human intestinal tract. It is also one of the most familiar organism in microbiology. Its presence in water and food are as fecal contamination. It’s not considered pathogenic, but could cause urinary tract infections, and produce

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