...Deleting Oncogenes in Cancer Cells with DNA Strand Slippage Abstract Oncogenes are DNA sequences which code for proteins that can cause cancer. Therefore, one way to treat cancer is to disrupt or destroy these genes. Lars Zender and colleagues recently discovered two genes that are the most likely causes of liver cancer (1). Zender claims oncogenes cIAP1 and Yap promote tumor growth, and are overexpressed in liver cancer cells. Garcia-Diaz and coworkers have also found that the slipping of DNA strands during synthesis by DNA polymerase can cause single bases to be deleted (2). Applying conditions for this slippage to the replication of the oncogenes in liver cancer cells will cause base deletion and render the gene unable to encode a protein that promotes tumor growth. The experimental control will be DNA sequences matching the oncogenes that will be treated with DNA polymerase λ due to its high rate of base deletion (2). A second experimental control will be DNA treated with DNA polymerase μ. The negative control will consist of untreated DNA. The crystallization process used by Garcia-Diaz and colleagues to uncover the structure of the polymerase and DNA will be used to determine which DNA bases, if any, are deleted. The oncogene will become unable to promote tumor malignancy if its bases are targeted for deletion. Background Zender applied genome analysis to human hepatocellular carcinoma cells, or liver cancer cells, and mouse liver tumors to determine...
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...Biochemistry GRT-1 (Roche Diagnostics PCR Diagnostic Kits, United States of America, 2014) The Role of DNA and RNA in the transmission of information within the cell Kristie French, BS, RN, PCCN Morganfield— Kentucky T: 270-952-5228 DD: 812-485-4649 E: kfrenc3@wgu.edu Summary This report has been prepared to discuss the role of DNA and RNA in the transmission of genetic information within the cell. The report is broken down into four sections in order to illustrate this information. Section 1 discusses and illustrates DNA replication at the biochemical level. This section includes a diagram that with representation of the components involved. Section 2 discusses and illustrates the role of the enzyme Ligase in the replication of DNA. Section 3 illustrates and discusses the role of mRNA in transcription and translation. Finally, Section 4 discussed the effects of RNA polymerase inhibition related to the poisonous effects of the death cap mushroom. Contents Summary 2 DNA Replication at the Molecular Level 4 Diagram 1: Process of DNA Replication at the biochemical level 4 Components of DNA Replication 4 The function of enzymes in DNA Replication 4 Enzymes involved in DNA Replication 4 The role of the ligase enzyme in the replication of DNA 5 Diagram 2: The role of the ligase enzyme in the replication of DNA 5 Components involved 5 The role of mRNA in transcription and translation 6 Diagram 3: The role of mRNA...
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...How DNA is packed in the nucleus of an eukaryotic cell 1) Introduction DNA, deoxynucleic acid, is known to be able to condense to form a smaller structure known as chromosomes. However, it is hard to imagine how does a 2m long DNA molecule can be packed into something which only have a rough diameter of 10µm in the eukaryotic cell. This report provides an overview on how DNA is packed in the nucleus of an eukaryotic cell. It will cover the structure of the human DNA, levels of packing the DNA and the necessity and benefits of DNA packing. 2) Structure of Human DNA The human DNA is made up of two polynucleotide strands wrapped around each other in a double helix structure. The two DNA strands in the double helix structure is oriented in opposite direction. A 3’ end of a DNA strand at the start of the strand has an OH group at its terminal, and ends with a 5’ end with a phosphate group attached to its terminal....
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...Explain the roles of mitotic cell division, meiosis, and fertilization in the human life cycle. Mitotic cell division allows for tissue repair/replacement and for an organism’s development from a fertilized egg (zygote) to an adult. Meiosis creates genetically variable gametes that each contain half the genetic material of a nonreproductive cell. The male gamete fertilizes the female gamete during sexual reproduction, forming a zygote with a full set of genetic material. 2. Why are both cell division and apoptosis necessary for the development of an organism? Cell division produces the new cells that make up an organism. Apoptosis (programmed cell death) carves out structures such as fingers and toes; it also protects an organism by eliminating damaged cells that might otherwise become cancerous. 8.2 1. Why does DNA replicate? DNA replicates before a cell divides so that each daughter cell receives a complete set of genetic information. 2. What is semiconservative replication? Semiconservative replication means that only one strand of a DNA molecule is newly formed. The other strand is original DNA from the parent cell. 3. What are the steps of DNA replication? The helicase enzyme unwinds and separates a double-stranded DNA molecule; binding proteins keep the strands separate. The primase enzyme forms a short stretch of complementary RNA on each DNA template. Starting at the end of the RNA primer, DNA polymerase adds DNA nucleotides that are complementary...
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...The difference between the cell cycles of a normal and cancerous cell is that the cancerous cell is uncontrolled when it reaches the cell division or anything else goes wrong threwout the cycle(can be very dangerous) and the normal cell is controlled throughout the whole cycle. In the g1 phase, the cell synthesizes mRNA and proteins in preparation for subsequent steps leading to mitosis. When the cell reaches the end of the G1 phase it is controlled at a checkpoint called G1/S, G1/S is when the cell decides whether or not to replicate its DNA. At the checkpoint the cell is checked for DNA. Cells with no damaged DNA (normal cells) continue to S phase, in S phase cells with damaged DNA (cancerous cells) that cannot be repaired die. G2 is when cell is about to divide. the M is...
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...DNA DNA or Deoxyribonucleic acid is the very building block needed in the creation of life. It is composed of genetic instruction to map out the creation of an entire organism. DNA is well suited in the storage of biological information. Yet with all of this complex information there are certain chemicals or forms of energy that can damage or alter this special coding. In process of damaging or altering the coding of DNA the most dreaded situation of a cancerous cell can be formed. To start let’s look at the different kinds of radiation that may or may not effect DNA. Alpha, Beta, and Gamma Radiation Ionizing Radiation can change the way our body’s work and develop. Direct includes covalent linkage between nitrogenous bases, and creating structure like thymine dimmers. Indirect is when it interacts with water to create free radicals which can interact with a person’s DNA. It can cause single or double stranded breaks in the sugar- phosphate backbone. (Buttons, n.d.) Alpha Radiation is a process that needs to be swallowed, inhaled, and or absorbed into the blood stream. This can increase the risk of cancer also known as lung cancer. Radon is a silent but deadly killer. To determine if one has been affected by alpha particles testing would be completed with special equipment and gear to prevent those from any exposure. Beta Particles can be a helpful process of treatment to many, such as treating thyroid disorders, cancer, and Graves’ disease (hyperthyroidism). It also...
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...Nitrogenous bases in DNA DNA is the principle molecule responsible for carrying genetic information within almost all living organisms for their development. They also provide some of the apparatus by which the instructions are carried out. This excludes viruses, as they use RNA, to carry their genetic information. (Image 1: DNA molecule, nucleotide, base pairing. Reference: http://www.britannica.com/EBchecked/topic/422006/DNA-sequencing ) Structurally, the DNA molecule consists of two intertwined strands, the margins of which are chains of sugar and phosphate groups. The chains are linked by pairs of substances called bases, of which there are: adenine, guanine, thymine and...
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...Importance of DNA - A T2 Assessment Understanding what DNA is, is very important. DNA is the material that carries all the information about how a human will look (facial features, etc,.) and also how they will process daily. For example, DNA in humans determines things such as what color the eyes are, what color the hair is, and also what gender you are when a baby. DNA is found in the nucleus of almost all cells. DNA also does something called replicating nucleotides. If DNA never replicated, cell life would be impossible. If this really were to happen, the cells would then die. DNA replicates itself in order to create more DNA strands for cells. It happens along the entire DNA molecule. It continues this until the entire strand/ladder...
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...Transformation Purpose Horizontal gene transfer refers to the transfer of genes from one organism to another through a method other than reproduction. Genetic transformation, a form of horizontal gene transfer, involves the altering of a cell through the uptake of naked DNA. Naked DNA refers to DNA which has been released from lysed or disrupted cells and is taken up by a recipient cell. If a cell is able to take up naked DNA, they are referred to as competent. This finding is accredited to Frederick Griffith, a bacteriologist who conducted an experiment in which a nonpathogenic strain of Streptococcus pneumoniae was exposed to a heat-killed pathogenic strain of the same bacteria. Griffith observed that although the virulent strain had been heat-killed; the DNA was able to survive the heating process and was taken up by the “resistant” strain of bacteria through gene transformation. In this lab, an experiment was conducted to test the hypothesis that a susceptible strain of bacteria, known as the S-strain, will grow in the presence of an antibiotic if it is combined with the DNA from the lysed pathogenic cell. Methods To demonstrate Griffith’s findings, an experiment was conducted with a competent prokaryotic cell known as Acinetobacter. This bacterium is Gram-negative, rod-shaped, and typically found in soil and water. During this experiment, three sessions were completed in order to observe whether gene transformation had successfully taken place. ...
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...capsid or envelope * nucleic acid: DNA or RNA, composed of single/double strand (genes encode coat proteins, proteins of regulation of transcription) * capsid: protein coat of virus, viral genome packaging * envelope: lipid bilayer that contains proteins * enveloped viruses: genomes contain genes for synthesis of envelope proteins * viral genomes= virus-specific enzymes (nucleic acid replication) * non-living organism/not “alive” - lack that properties of life (no independent reproduction, no metabolic system= no energy, dependent on host cells 2. Why viral infections are usually difficult to treat with drugs, and exceptions to this general principle. * viruses= hidden in host cells, use host cell machinery to replicate (no obvious viral product to be targeted by drugs/antibiotics - not like bacteria) * symptoms can only be relieved not treated - natural immune function treats — some viruses deadly symptoms= prevention w/ vaccinations (e.g. measles, polio) * Viruses using own polymerases (e.g. RNA viruses - influenza)= more obvious targets - antiviral drugs to treat 3. Whether viruses are always pathogenic. * not all viruses= pathogenic - some benefit hosts e.g. some non-pathogenic viruses protect humans from pathogenic viruses * “protective” viruses - interfere with replication/functions of pathogenic viruses * some viruses defend host cells (e.g. bacteriophages/phages control bacteria...
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...|Do-It-Yourself Strawberry DNA LAB | |Introduction: [pic] | |Since DNA is the blueprint for life, everything living contains DNA. DNA isolation is one of the most basic and essential | |techniques in the study of DNA. The extraction of DNA from cells and its purification are of primary importance to the field of | |biotechnology and forensics. Extraction and purification of DNA are the first steps in the analysis and manipulation of DNA that | |allow scientists to detect genetic disorders, produce DNA fingerprints of individuals, and even create genetically engineered | |organisms that can produce beneficial products such as insulin, antibiotics, and hormones. | |DNA can be extracted from many types of cells. The first step is to lyse or break open the cell. This can be done by grinding a | |piece of tissue in a blender. After the cells have broken open, a salt solution such as NaCl and a detergent solution containing | |the compound SDS (sodiumdodecyl sulfate) is added. These solutions break down and emulsify the fat & proteins that make up a cell| |membrane. Finally, ethanol is added because DNA is soluble in water. The alcohol causes DNA to precipitate, or settle out of the | |solution, leaving behind all the cellular...
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...in this process - translation 3) Codons are involved - translation 4) Uses DNA Polymerase- replication 5) Polymerase chain reaction is a “synthetic” version of this 6) RNA polymerase is used - transcription 7) Ribosomes are used- translation 8) tRNA is used - translation 9) mRNA is produced - transcription 10) mRNA is read - translation 11) Important when a cell divides- replication 12) Uses a start codon - translation 13) A stop codon is involved - translation 14) Ends with a termination sequence - translation 15) Begins at a promoter region - transcription 16)What are the two main types of cells? * Eukaryotes: animals, plants, yeast, algae, most multicellular organisms; yes nucleus * Prokaryotes: bacteria, archaebacteria, simpler organisms; no nucleus; no membrane enclosed organelles 17) Approximate size scale of bacterial cell (1 micron) vs. animal cell (10-100 microns) vs. molecules vs. virus 18) How does the cell fit 6 feet of DNA into each cell of our body? 19) How many chromosomes do humans have? What’s the difference between male and female chromosomes? 23; XX (female) versus XY (male) 20) Plasmids are the most important entity for biotechnology. They allow the insertion of foreign DNA 21) What was the first protein biotechnology to be produced in E. coli? 22) DNA: fingerprint of cell; coding material for everything in our body; way we look and act; genetic material...
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...DNA is known to be the most common unit of genetic material in life on Earth; however, Genetic information is stored within the chemical structure of the DNA ,the two backbones, which form double helix , a chain of four chemicals which called bases , and they are running along the backbones , as well as base pairs which are Bridges between bases that located on opposite backbones , The four bases are indicated by the letters A, T, C and G, and their order form a genetic "code" , A,T,C,G can be called triads or codons ,they carry a specific code or message that cells can understand ,and each code or message tells the cell to make for examples black hair, or make green eyes or help a person to grow tall or shorter, however ,DNA molecules are able to make copies of themselves during cell division or when the cell reproduces, usually the DNA molecules contains two identical molecules that were produced from one single parent molecule. When fertilizing an egg cell with a sperm cell, the sperm gives DNA to the fertilized egg and the egg has its DNA already , as we know...
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...* DNA technology – Methods for studying and manipulating genetic material, has rapidly revolutionized the field of forensics, the scientific analysis of evidence for legal investigators * Has practical applications beyond its use in forensic science * Include the use of gene cloning in the production of medical and industrial products, the development of genetically modified organisms for agriculture, and even the investigation of genealogical questions * DNA evidence was used to solve a double murder in England * Showed that two murders could have been committed by the same person * Showed the innocence of someone who confessed to one of the murders * Showed the absence of a match in 5,000 men tested when the murderer persuaded another man to donate blood in his name * Showed a match with the murder and DNA found with both victims * Biotechnology: The manipulation of organisms or their components to make useful products * Grew out of discoveries made about 60 years ago by American geneticists Joshua Lederberg and Edward Tatum * Performed a series of experiments with E.coli that demonstrated that two individual bacteria can combine genes * Genetic engineering involves manipulating genes for practical purposes * Gene cloning leads to the production of multiple identical copies of a gene-carrying piece of DNA * Recombinant DNA is formed by joining DNA sequences from two different sources ...
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...genetic material during cell division. Horizontal gene transfer, on the other hand, is the transfer of genetic material between different species of bacteria; three types are found. Conjugation is the transfer of DNA by direct contact with the cell. Transformation is the uptake of genetic material from the surrounding. Transduction is the transfer of genes via bacterophages. Two kinds of bacterial transductions can be found: generalized and specialized. Viruses are unable to replicate on their own. To do so, they infect a cell and force it to make copies of itself. In the case of generalized transduction, a virulent phage infects the bacterial cell by attaching to a receptor area and injecting its nucleic acid into the cell. The host DNA is broken into smaller fragments, replicated and translated. When phages are assembled, they occasionally carry a piece of the bacterial DNA picked at random. Any segment of the bacterial DNA can be assembled in the new phage. The cell then enters the lytic phase, releasing the phage. This phage then infects another cell, transferring the bacterial DNA which recombines with the host’s chromosome. In specialized transduction, only a few specific genes are transferred from one bacterial cell to another and it requires a certain type of phage, called temperate bacteriophage. When it infects the cell, its DNA enters the cell and binds to a specific site on the host chromosome, producing a lysogenic cycle. This integrated DNA can remain for long periods...
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