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Ergosterol Essay

Submitted By
Words 1482
Pages 6
BY:
Esa Girish Patel
Student No: 104668544
Course: Biochem and Cell biology- Lipids & Membranes
Instructor: Dr. Anna Kozarova

INTRODUCTION:

Ergosterol (ergosta-5, 7, 22-trien-3β-ol) is an isoprenoid compound (8) and a basic sterol found in cell membranes of fungi and protozoa that are considered as “Fungal sterol” (1). Ergosterol was discovered 125 years ago in plant pathogenic Ergot fungus Claviceps purpurea(1).Ergosterol is a characteristic component of the fungal cell membrane and is completely or nearly absent in animal, plant and bacterial cells (2).Ergosterol accounts for (depending on growth phase) 10–80 % of all sterols present in the mycelium (3). It forms a part of the sterol mixture of primitive Tracheophyte, Lycopodium …show more content…
In records there are various methods used for detection and purification of ergosterol like reversed phase High pressure liquid chromatography (HPLC) and ultraviolet detection (HPLC-UV) (13),thin layer chromatography, solid phase extraction, formation of sterol derivatives and their analysis using capillary gas chromatography or gas liquid chromatography (13). Other techniques for determination was non-discriminating flash pyrolysis with as gas chromatography/mass spectrograph which had an advantage like lacking sample pretreatment and very small sample size which was required for analysis …show more content…
Polyene antibiotics like AmphotericinB, Nystatin, and Natamycin complex with ergosterol in the plasma membrane, causing membrane disruption, increased permeability, leakage of cytoplasmic contents which leads to cell death(17). Azole derivatives acts on ergosterol biosynthesis pathway at the C-14 demethylation stage, a three step, oxidative reaction catalyzed by the cytochrome P-450enzyme 14a-sterol demethylase (P-450DM) which leads to accumulation of lanosterol results in damage of plasma membrane leading to cell death (17). In case of Allylamines they act as noncompetitive inhibitors of squalene epoxidase an enzyme which, together with (2, 3)-oxidosqualenecyclase, is responsible for the cyclization of squaleneto lanosterol resulting in ergosterol depletion with more accumulation of squalene which affects membrane structure and function

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