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Ethical Treatment of Animals

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Genetically Modified Animals
Donna K. Lane
BIO1000
Dr. Straub
Capella University
September, 2014

The majority of genetically modified animals are used in research to explore changes in Phenotype when specific genes are altered. Genetically engineered animals will improve human health through production of replacement proteins, drugs, vaccines, research models and tissues for the treatment and prevention of human disease.
Genetic modification can produce animals that are susceptible to certain compounds or stresses for testing in biomedical research (Edge, A.S. 1998). Some genetically modified animals are used as models of human diseases and potential treatments and cures can first be tested on them. Other animals have been engineered with the purpose of potentially increasing their use in medicine and industry.

Genetic engineering is a useful technology because it enables animals to produce useful proteins used in research. Conventional animals breeding is constrained to selection based on naturally-occurring variations in the proteins that are present in a species, and this limits the range and extent of genetic improvements that can be achieved (Wagner, J. 2001).

When genetically modified animals are produced, they are produced for two distinct reasons: human medicine and agriculture. Most commercial transgenic animal research is in the field of human medicine. Many therapeutic proteins for the treatment of human diseases require animal-cell specific modifications to be effective, and at the present time they are almost all produced in mammalian cell-based bioreactors (Edge, A.S. 1998).

Transgenic animals are also being used to produce serum biopharmaceutical products such as antibodies that can be used for the treatment of infections, cancer, organ transplant rejections, and autoimmune diseases such as Rheumatoid Arthritis.

Genetic engineering require three elements: the gene to be transferred, a host cell into which the gene is inserted, and a vector (any organism that will carry the gene from one place to another) to bring about the transfer. The procedure for genetically modified Insulin is:

Researchers obtain a copy of the Insulin gene from a natural source from the DNA in a pancreas or from some that has been manufactured in a laboratory.

The second step is to insert the Insulin gene in to the vector. The most common vector used in genetic engineering is a circular form of DNA known as Plasmid. Endonucleases are used to cut the Plasmid molecule open at any point chosen by the scientist. Once the Plasmid has been cut open, it is mixed with the Insulin gene and a Ligase Enzyme. The goal is to make sure that the Insulin gene attaches itself to the Plasmid before the Plasmid is closed.

The hybrid Plasmid now contains the gene whose product (Insulin) is desired. It can be inserted into the host cell, where it begins to function just like all the other genes that make up the cell. In addition to normal bacteria functions, the host cell is also producing Insulin. This process is referred to as recombinant DNA (rDNA) research (Edge, A.S. 1998).

While the agricultural use of genetic modification is convincing, the medical aims are ground-breaking.

With the needs of both public health and food security, genetically engineered animals promise not only safer, lower cost proteins and drugs that could increase necessary changes in medical practices, but also better medical products that will provide improvements in today’s medicines.

The drugs that genetically modified animals can produce – blood components, replacement proteins, antibodies, and xenotransplants remain among the most expensive drugs to produce in the world. Genetically engineered animals can deliver substantial improvements in terms of cost, safety and availability of urgently need drugs and treatments, thus bringing substantial public health benefits (Wagner, J. 2001).

Researchers have already produced Insulin, growth hormones and blood anti-clotting factors from the milk of genetically engineered Cows. Research is still ongoing to manufacture milk, through Transgensis, from genetically modified Cows, Sheep/Goats, for the treatment of debilitating disease such as Phenylketonuria (PKU), hereditary Emphysema, and Cystic Fibrosis. Scientists are also doing research on genetically engineered Pigs in the hopes of one day being able to produce human organs needed for transplants, such as heart, liver and kidney.

It never ceases to amaze me the wonderful advancements Science can provide us with. Maybe with all the research that is going on around the world, one of those scientists can finally discover the cure for Cancer and the other horrible life taking diseases.

References:

Edge, A.S., (1991). “Xenogenic cell therapy: Current progress and future developments in porcine cell transplantations”. Cell Transplantation, 7(6): 525-868. Doi:10.1016/SO963-6897 (98) 00043-8.

Wagner, J. (1995). “Transgenic animals are models for human disease”. Clin. Exp. Hypertens. 17(4): 356-454. Doi: 10. 3109/1064. 1969509037410.

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