HDAC Inhibitor-loaded Bone Cement for Advanced Local
Treatment of Osteosarcoma and Chondrosarcoma
What type of drug did they use?
In the experiment, the researchers used two anti-neoplastic HDAC inhibitors, valproic acid (VPA) and suberoylanilide hydroxamic acid (SAHA)
What was the composition of the cement?
The bone cement consists of 90% polymethylmethacrylate (PMMA) and 10% of contrast media crystals. There are two substances make the structure of this particular bone cement, small particles of pre-polymerized PMMA (white powder), and MMA (a liquid monomer). These two substances are combined in a polymerization reaction to form a net-like structure.
Method of incorporating the drug, in dry powder or liquid?…show more content… In this case, they prepared the bone cement clots of 5g and loaded with two different concentrations of VPA, 25mg and 50 mg and three different concentrations of SAHA, 1 mg, 2.5 mg and 5 mg.
Was the cement injectable and how did they test it?
No, the cement was made with powder and shaped of a tablet which was used for systemic therapy. So, the bone cement clots of 5g were embedded with different drug concentrations in 10 ml of DMEM/F12 cell culture medium. Medium from the clot was taken out and collected at −20˚C until processing to three different cell lines, hMSC, SW1353 and SaOs-2 cells. Right after removing the medium, the clots were embedded in 10 ml of DMEM/F12. These entire steps were followed on days 2, 3, 4 and 7. Drug elution rate into the medium was measures for VPA but not for SAHA.
Did they run any in vitro or in vivo toxicity test? If yes what are they.
Yes, the ran in vitro toxicity text using an Alamar Blue assay on days 1, 2, 3, 4 and
