Enzymes are biological catalysts that carry out thousands of chemical reactions that occur in living cells. Most of them are proteins. They speed up metabolic reactions in the body but remain chemically unchanged themselves In the experiment, the enzyme amylase was involved.this enzyme takes part in the digestion ofcarbohydrate. digestion is the break down of large complex and insoluble food into small simple soluble molecules for the body to absorb it. starch is a complex, the body have to find a way to break down starch, so the body produces salivary amylase in the mouth to begin the break down of starch into maltose.
As the temperature rises, reacting molecules have more and more kinetic energy. This increases the chances of a successful collision and so the rate increases. There is a certain temperature at which an enzyme's catalytic activity is at its greatest . This optimal temperature is usually around human body temperature (37.5 oC) for the enzymes in human cells. Above this temperature the enzyme structure begins to break down (denature) since at higher temperatures intra- and intermolecular bonds are broken as the enzyme molecules gain even more kinetic energy.Also Enzymes have an active site. This is part of the molecule that has just the right shape and functional groups to bind to one of the reacting molecules. The reacting molecule that binds to the enzyme is called the substrate.At high temperature,the active site is said to be denatured.Therefore,the active site cannot bind with any subtrate anymore.Amylase breaking down the starch suspension into maltose and maltose into glucose when HCL was added into solution B .This is because solution B was hydrolyzed and the H+ ions present break down the bond in between molecules of the solution B The HCl will denature (unfold) the amylase (as noted at the bottom of the link you provided), disrupting