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Jejunum Lab Report

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Objective: Investigate the influence of ethanol, polyethylene glycol 400, propylene glycol, glycerol and sorbitol on the absorptive clearance of ketotifenfumarate in the rabbit.
Methods:In situ intestinal perfusion technique, ̎through and through ̎ was used for estimation of membrane transport parameters of ketotifenfumarate from duodenum, jejunum, ileum and ascending colon in the rabbit. These parameters include absorptive clearance per unit length PeA/L (ml/min.cm), percentage fraction absorbed per unit length ( %Fa/cm) and anatomical length required for complete absorption in specific segment (L95%).
Results: The absorption was in the order ascending colon> duodenum > jejunum> ileum; where the absorptive clearance normalized to intestinal segment length PeA/L (ml/min.cm) was 0.0071±0.0003, 0.0058±0.0001, 0.0051±0.0001, and 0.0047±0.0001 in each segment respectively. The effect of cosolvents in jejunum was in the order; ethanol 15% >glycerol 30% > propylene glycol (PG40%) > polyethylene glycol400 (PEG-400 40%) >sorbitol 40%, Where the absorptive clearance normalized to intestinal segment length PeA/L (ml/min.cm), mean ± SE was 0.0142 ±0.0011, 0.0086 ± 0.0002, 0.0075 ±0.0003, 0.0022 ± 0.0001, and 0.0014±0.0001 for each cosolvents respectively. The same order was obtained in the ascending colon. …show more content…
It is available in two forms: ophthalmic form to treat allergic conjunctivitis, and in oral form to prevent asthma attacks2.Ketotifen is almost completely absorbed from the gastrointestinal tract following oral administration; its oral bioavailability is reported to be only about 50 % due to hepatic first-pass metabolism. Ketotifen N-glucuronide was reported to be the major metabolite, which is excreted in urine and feces as 50 % of dose

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