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Flocculation- a colloid phenomenon in which the disperse phase separates in discrete, usually visible, particles rather than congealing into a continuous mass, as in coagulation.
Acrolein test- Acrolein is the simplest unsaturated aldehyde.It is produced widely but is most oftenimmediately reacted with other products due toits instability and toxicity. It has a piercing,disagreeable, acrid smell similar to that of burning fat, it can be produced by heatingglycerol to 280 Celsius or in this case, by reactingpotassium bisulfate with glycerol. The test thatdifferentiates between cholesterol and lecithin isthe acrolein reaction. When lipids containingglycerol are heated in the presence of potassiumhydrogen sulfate, the glycerol is dehydrated,forming acrolein, which has an unpleasant odor.Further heating results in polymerization of acrolein, which is indicated by the slightblackening of the reaction mixture. Both thepungent smell and the black color indicate the presence of glycerol and therefore fat and/orlecithin. Cholesterol gives a negative acroleintest.Illustrated below is the conversion of glycerol intoacrolein using potassium bicarbonate. Acroleine can be characterized as a substance thatproduces a burnt fat odor.
Salkowski’s test- A Salkowski's test is a test for cholesterol. When concentrated sulfuric acid is added to a chloroform solution of cholesterol, the chloroform layer shows a red to blue colour and the acid layer shows a green fluorescence. This test is named after a German chemist by the name of Ernest Leopold Salkowski.
Leibermenn-Burchard Test- The principle involved in the Liebermann Burchard test for cholesterol is that cholesterol reacts when exposed to acid. This method shows the presence of cholesterol by the sample changing colors. The cholesterol changes to a blue-green color. *

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