Lab 1: Microscopy and the Metric System
Purpose
The purpose of this lab is to become familiar with the lighted microscope, how to prepare a wet mount, and understand taking measurements in the metric system and its conversions.

Materials & Methods Materials:
Part A: * Computer for videos

Part B: * Tape measure with centimeters * Scale that measures grams * Thermometer that shows Celsius * Campbell Biology Book * 1 piece Orange Starburst

Methods: Part A: Combined Lighted Microscope 1. Watch video on microscope 2. Answer the provided questions on the microscope
Wet Mount: 1. Watch video on wet mount 2. Explain the process of creating a wet mount Part B: Width of Book: 1. Lay book flat 2. Take the measuring tape and measure the width of the book in centimeters
Mass of Orange Starburst: 1. Place the scale on flat surface 2. Zero the scale and set the display to grams 3. Place the orange starburst and write down the mass in grams
Meniscus:
1. Answer the provided question on Meniscus in a graduated cyclinder
Temperature:
1. Set thermometer to display temperature in Celsius 2. Take room temperature by turning on the thermometer and noting temperature 3. Take body temperature by placing the thermometer on the back of your hand

Results
Part A: Microscopy 1) Parts of a compound microscope and their functions. a) Focal Adjustment- helps adjusts the slide into focus b) Eyepiece- the lens that you look into to see what is on the slide c) Objective lenses- the lens closest to the slide d) Stand- Holds all the parts together e) Light Source- located under the area where you put the slide and helps illuminate the slide for better observation f) Base- bottom of the microscope g) Arm- how you carry the microscope and it attaches the tube to the base 1) Determine the total magnification given that you are using a compound microscope with the following objectives: 4X, 10X, 40X, and 100X.
**With a 10x viewing lens h) 4x- 40x i) 10x- 100x j) 40x- 400x k) 100x- 1000x 2) What is meant by the depth of field? l) How sharp you can view the object on the slide 3) What is meant by the field of view? m) The amount you can see on the slide without moving it. 4) Describe the process of making a wet mount. n) Wet Sample- First you take the dropper to pick up the sample material and apply a small amount on the glass slide. Then take a cover glass and carefully lower it on top of the sample. Also if there is excess water, use filter paper to absorb it. And if not enough water then place a small amount on the edge of the cover glass and it will suck it under the cover glass. o) If the sample is too big- Place two drops of water on each end of the slide and place a cover glass on top of each drop. Then place a cover glass on top of the ends of the two cover glasses. Then place the sample under the third cover glass with a pipette. Also if there is excess water, use filter paper to absorb it. And if not enough water then place a small amount on the edge of the cover glass and it will suck it under the cover glass. p) Dry Sample- First to place some water (or immersion oil) on the slide and then put the dry sample on top of it. Then you place more water on top of the dry sample. Also if there is excess, water use filter paper to absorb it. And if not enough water then place a small amount on the edge of the cover glass and it will suck it under the cover glass.
Part B: Metric System 1) Linear measurements: Measure the width of your textbook in cm and then convert to mm. | Width in cm | Width converted to mm | Campbell Text | 24.5 cm | 245 mm | 2) Conversions: Convert 100 grams to mg and then μg | Equivalent in mg | Equivalent in μg | 100 g | 100,000 mg | 100,000,000 μg |

3) Weight measurements: Using your scale, record the mass of an object in grams and include the name of the object you have measured. Once you have recorded your measurement in grams, please convert that measurement to mg and then μg. | Weight in g | Weight in mg | Weight in μg | 1 orange Starburst | 5.4 g | 5,400 mg | 5,400,000 μg | 4) Volume measurements: Define meniscus and describe how you would read the volume of a liquid in a graduated cylinder. a) Meniscus is the convex or concave curve in the upper surface of a liquid caused by surface tension. b) You would read the volume of a liquid in a graduated cylinder by placing top part of the liquid at eye level then reading the number closest to the bottom curvature of the meniscus. 5) Temperature measurements: Record the temperature of your skin and of the room in °Celsius. | Temperature in Celsius | Room Temperature | 25.4 c | Skin Temperature | 32.5 c |