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Microbiology
Final exam
Study reference

1. Gram Stain

* Verify if bacteria are present or not.
* Controls – positive (purple) – S.aureus

* negative ( red/pink) – E.coli

2. Endospore Staine
Positive controller – B. magneterium
Green spore- Positive
Pink (vegetative ) – Negative

3. Acid fast

Positive control – M. smeagmatis

Blue – negative

Pink /red- positive

4. Motility
Positive control - P.vulgaris A. non motile is negative test B. motile is a positive test

5. Carbohydrates fermentation (test for gram -)
( glucose , manitol, lactose)
Positive control – ecoli (yellow)
Red- no color change – negative test
Yellow – color change – positive test

6. Mae Conkey’s Agar
Ecoli – positive control
Selective for negative gram stain
Differential for organism that could ferment lactase
Growth pink – positive
No growth – negative

7. Gelatin Hydrolysis ( Gelatinase)
Positive control – P.aeruginosa
Liquid –positive test
Solid - Negative test

8. Blood agar
Positive control – S. aureus

A. Betahemolysis
B. alphahemolysis
C. gammahemolysis

9. FTM
*broth – O2 relationship with

10. MRVP (mix acid fermentation)
MR- methyl red (PH lower than 4.4)
Positive control – e.coli red color- positive test no red – negative test

11. Voges Proskauer ( acetone) –precursor for those who fermented butane
Positive control- E. aerogenosa Red color – positive test No color change – negative test

12. Nitrate Reduction
Enzyme – nitrate redactase
Positive control- E.coli
Pink color- positive
No pink – negative

13. Starch hydrolysis (iodine), test for enzyme laminase
Positive control- B. subtillus Zone of clearing- positive test
No zone of clearing – negative test

14. Fat hydrolysis, test for enzyme lipase
Positive control –

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