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You are given a sputum sample from a patient that may be infected with a bacterium from one of the following genera: Bacillus, Escherichia, or Mycoplasma. In a Microsoft Word document, describe the important anatomical differences among these three genera (i.e., those features that could be used to differentiate among them) and the staining protocols you would use to identify which genera is causing the patient’s infection. When discussing each staining protocol, it is important not only to mention the steps involved, but also how each step and each protocol would identify or eliminate each of these genera as a suspect. You may refer to other scientific resources, but they should be in addition to and not in place of the Module 2 resources.

Bacillus: Rod Shaped Bacteria, plural bacilli, Divide only across their short axis, most appear in single rods. Diplocacilli appear in pairs after divison, and streptobacilli occur in chains. Some bacilli look like straws, others are tapered and look like cigars and some are oval and look similar to cocci so they are called coccobacilli.

Genera Bacillus contains bacteria such as Bacillus anthracis the bacteria that causes anthrax. Bacillus cells often form long, twisted chains of cells. Gram POSITIVE, can be aerobic or anaerobic. Prokaryotes, Has a cell wall

Mycoplasma: Lack a cell wall Unaffected by many common antibiotics such as penicillin or other beta-lactam antibiotics that target cell wall synthesis. May be parasitic or saprotrophic, can cause pneumonia and other respiratory disorders also M. genitalium which may cause pelvic inflammatory diseases. Smallest bacterial cells discovered can survivie without oxygen and are 0.1um in diameter usually. Divide by binary fusion, prokaryote, Usually have 3 organelles: cell membrane, ribosomes and circular double stranded DNA. Gram NEGATIVE, fried egg

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