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Microbiology Semester Test 244

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VOORLETTERS EN VAN/INITIALS AND SURNAME:…………………………………………..

STUDENTE NOMMER/STUDENT NUMBER:………………………………………….…………...

GEEN SAKREKENAARS WORD TOEGELAAT NIE
NO CALCULATORS ARE PERMITTED

|UNIVERSITEIT VAN STELLENBOSCH |UNIVERSITY OF STELLENBOSCH |
|DEPARTEMENT MIKROBIOLOGIE |DEPARTMENT OF MICROBIOLOGY |
|SEMESTERTOETS 2012 |SEMESTER TEST 2012 |
|MIKROBIOLOGIE 244 |MICROBIOLOGY 244 |
| |
|Punte/Marks: 120 |Tyd/Time: 2 uur/hours |

Beantwoord alle vrae. Beantwoord Afdeling A op die vraestel en beantwoord Afdeling B in die eksamenboek.
Answer all the questions. Answer Section A on the questionnaire and answer Section B in the exam book.
AFDELING A/SECTION A

Omkring die letter wat ooreenstem met die korrekte antwoord by Vrae 1 tot 30. Vul die korrekte antwoord in vir Vrae 31 en 32. / Circle the letter corresponding to the correct answer in Questions 1 to 30. Fill in the correct answer for Questions 31 and 32.

1. Watter organelle sal u verwag om in die sitoplasma van ‘n tipiese fungus aan te tref? / Which organelles would you expect to find in the cytoplasm of a typical fungus? a. chlamydospore / chlamydospores b. mitochondrië / mitochondria c. tilakoïed-membrane / thylakoid membranes d. chlorosome / chlorosomes

2. Dormante, dikwandige rustende selle van sianobakterieë wat weerstandig is teen uitdroging, word genoem _______. / Dormant, thick-walled resting cells of cyanobacteria that are resistant to desiccation are called ______.
a. baeosiete / baeocytes.
b. akinete / akinetes.
c. hormogonia / hormogonia.
d. tilakoïede / thylakoids.

3. Archaeiese membrane bevat die volgende nie-polêre lipiede. / Archaeal membranes contain the following non-polar lipids.
a. isopreniel gliserol eters / isoprenyl glycerol ethers
b. gliserol vetsuur esters / glycerol fatty acid esters
c. o-nitrofeniel-(-D-galaktopiranosied / o-nitrophenyl-(-D-galactopyranoside
d. Al bogenoemde / All of the above

4. Aktinomisete vorm / Actinomycetes form
a. substraatmiselia en lugmiselia. / substrate mycelia and aerial mycelia.
b. hittebestande endospore. / heat resistant endospores.
c. prosteka met aanhegtingspunte. / prostheca with holdfasts.
d. ‘n skede gemaak van polisakkariede en proteïene. / a sheath made of polysaccharides and proteins.

5. Clostridium perfringens veroorsaak watter van die volgende? / Clostridium perfringens causes which of the following?
a. gas gangreen / gas gangrene
b. longontsteking / pneumonia
c. “fire blight disease” by pere / fire blight disease of pears
d. tuberkulose / tuberculosis

6. Lede van die die genus Neisseria veroorsaak watter van die volgende menslike siektes? / Members of the genus Neisseria cause which of the following human diseases?
a. gonorree / gonorrhea
b. sifilis / syphilis
c. genitale herpes / genital herpes
d. vaginitis / vaginitis

7. Watter van die volgende word gebruik in die produksie van karringmelk en kaas? / Which of the following is used in the production of buttermilk and cheese?
a. Streptococcus pneumoniae
b. Streptococcus mutans
c. Streptococcus fecaelis
d. Lactococcus lactis

8. Metanogeniese Archaea is / Methanogenic Archaea are
a. verpligte anaerobe wat metaan produseer / obligate anaerobes that produce methane.
b. verpligte anaerobe wat metaan verbruik / obligate anaerobes that consume methane.
c. verpligte aerobe wat metaan produseer / obligate aerobes that produce methane.
d. verpligte aerobe wat metaan verbruik / obligate aerobes that consume methane.

9. Lede van die genus Pseudomonas / Members of the genus Pseudomonas
a. is aerobe wat ‘n wye verskeidenheid organiese verbindings mineraliseer. / are aerobes that mineralize a wide variety of organic compounds.
b. benodig ‘n hoë konsentrasie organiese materiaal vir groei. / require a high concentration of organic material for growth.
c. vorm siste wanneer onvoldoende hoeveelhede voedingstowwe gegee word. / form cysts when starved for nutrients.
d. voer anaerobiese fotosintese uit. / perform anaerobic photosynthesis.

10. Bakterieë wat simbioties binne die wortelknoppie-selle van peulplante groei, waar hulle as bakteroïede stikstof fikseer, behoort tot die genus _____________. / Bacteria that grow symbiotically within the root knob cells of legumes, where they fix nitrogen as bacteroids, belong to the genus ____________.
a. Rickettsia
b. Nitrobacter
c. Rhizobium
d. Nitrosomonas

11. Watter een van die volgende organismes veroorsaak pes? / Which one of the following organisms causes bubonic plague?
a. Bordetella pertussis
b. Yersinia pestis
c. Clostridium tetani
d. Campylobacter jejuni

12. Die genus Mycobacterium / The genus Mycobacterium
a. is streng anaerobe en katalase positief. / are strict anaerobes and catalase positive.
b. het selwande soortgelyk aan gram-negatiewe bakterieë. / have cell walls similar to gram negative bacteria.
c. kleur suurvas. / stain acid-fast.
d. beide a en b. / both a and b.

13. Watter van die volgende word tans gebruik of word ondersoek vir gebruik as ‘n biologiese insekdoder? / Which of the following is currently being used or investigated for use as a biological insecticide?
a. Bacillus cereus
b. Bacillus subtilis
c. Bacillus anthracis
d. Bacillus thuringiensis

14. Microsporidia is ______. / Microsporidia are _______.
a. bakterieë met baie klein spore / bacteria with very small spores.
b. fungi wat verpligte intrasellulêre parasiete van diere is / fungi that are obligate intracellular parasites of animals.
c. askomisete fungi met klein spore. / ascomycetous fungi with small spores.
d. prokariotiese fungi / prokaryotic fungi.

15. Watter Streptococcus species veroorsaak keelseer? / Which Streptococcus species causes sore throat (strep throat)?
a. Streptococcus pyogenes
b. Streptococcus mutans
c. Streptococcus throatus
d. Streptococcus pneumoniae

16. Geslagtelike spore wat deur fungi gevorm kan word sluit in _____. / Sexual spores that may be formed by fungi include _____ .
a. Beide sporangiospore en konidiospore. / Both sporangiospores and conidiospores.
b. Beide sigospore en arthrospore. / Both zygospores and arthrospores.
c. Beide askospore en chlamydospore. / Both ascospores and chlamydospores.
d. Beide sigospore en basidiospore. / Both zygospores and basidiospores.

17. Lede van die genus Mycobacterium veroorsaak watter van die volgende siektes in mense? / Members of the genus Mycobacterium cause which of the following diseases in humans?
a. tuberkulose en melaatsheid / tuberculosis and leprosy
b. absesse /abscesses
c. diphtheria / diphtheria
d. meningitis / meningitis

18. Die spirochete sluit in organismes wat _______ veroorsaak./ The spirochetes include organisms that are the causative agents of _____.
a. sifilis / syphilis.
b. Q-koors / Q fever.
c. pes / plague.
d. kinkhoes / whooping cough.
19. Lede van die die genus Vibrio veroorsaak watter van die volgende menslike siektes? / Members of the genus Vibrio cause which of the following human diseases?
a. gonorree / gonorrhea
b. sifilis / syphilis
c. cholera / cholera
d. meningitis / meningitis

20. Wat is die korrekte skryfwyse vir ‘n bakteriese spesie-naam wat die eerste keer in die teks van ‘n dokument genoem word? / Which is the correct way to write a bacterial name that is mentioned for the first time in the text of a document?
a. Escherichia coli
b. Escherichia Coli
c. E. coli
d. Escherichia coli

21. Watter een van die volgende groepe Archaea benodig hoë konsentrasies sout om in te groei? / Which one of the following groups of the Archaea requires high concentrations of salt to grow in?
a. Halobakterieë / halobacteria
b. Metanogene / methanogens
c. Termococci / thermococci
d. Geen van bogenoemde nie / none of the above

22. Watter van die volgende is die naaste verwant aan Clostridium? / Which of the following is the closest related to Clostridium?
a. Crenarchaeota
b. Aspergillus
c. Amanita phalloides
d. Serratia

23. Lede van die genus Leuconostoc is belangrik in die produksie van ____ . / Members of the genus Leuconostoc are important in the production of_____.
a. dekstrane / dextrans.
b. suurkool /sauerkraut.
c. kaas /cheese.
d. al bogenoemde / all of the above.

24. ‘n Eensellige fungus wat ongeslaglik deur botselvomring of selsplytsing, of geslagtelik deur spoorvorming voorplant, word ‘n _______ genoem. / An unicellular fungus that reproduces either asexually by budding or fission, or sexually through spore formation is called a ________.
a. ligeen / lichen
b. parafiletiese fungus / paraphyletic fungus
c. spoorvormende fungus / sporeforming fungus
d. gis / yeast

25. Beide endogeniese geslagtelike spore en eksogeniese ongeslagtelike spore word geproduseer deur die _____ . / Both endogenic sexual spores and exogenic asexual spores are produced by the _____ .
a. Crenarchaeota.
b. Zygomycota.
c. Basidiomycota.
d. Ascomycota.

26. Wanneer sommige Streptococcus spesies op bloedagar gegroei word, toon hulle onvolledige hemolise met die vorming van ‘n groenerige sone rondom die kolonie; hierdie staan bekend as____. / Some species of Streptococcus, when grown on blood agar, produce incomplete hemolysis indicated by the formation of a greenish zone around the colony; this is referred to as_____.
a. (-hemolise / alpha-hemolysis b (-hemolise / beta-hemolysis
c. (-hemolise / gamma-hemolysis
d. geen van hierdie / none of these

27. Morfovars is stamme van ________ . / Morphovars are strains from _______ .
a. verskillende spesies waarvan die morfologie ooreenstem. / different species with similar morphology.
b. dieselfde spesies waarvan die morfologie verskil. / the same species which differ in morphology.
c. verskillende spesies waarvan die antigeniese eienskappe ooreenstem. / different species with similar antigenic properties.
d. dieselfde spesies waarvan die fisiologiese eienskappe verskil. / the same species which differ in physiological properties.

28. Die temperatuur waarby die twee stringe van ‘n dubbelstring DNA molekule van mekaar sal skei, staan bekend as die __________ temperatuur. / The temperature at which the two strands of a double-stranded DNA molecule will separate from each other is called the __________ temperature.
a. skeidings / separation
b. smeltings / melting
c. destabiliserings / destabilizing
d. mobiliserings / mobilization
29. Aktinomisete spore wat binne spesiale strukture gevorm word, word ________ genoem. / Actinomycete spores formed within a special structure are called ________.
a. endospore / endospores.
b. konidiospore / conidiospores.
c. sporangiospore / sporangiospores.
d. miksospore / myxospores.

30. Sferiese selle van sianobakterieë wat atmosferiese stikstof kan bind, word na verwys as ______ . / Spherical cells of cyanobacteria that are able to fix atmospheric nitrogen are referred to as _______.
a. heterosiste. / heterocysts.
b. mikrosiste. / microcysts.
c. öosiste. / oocysts.
d. nitrosiste. / nitrocysts. (30)

VRAAG 31 / QUESTION 31

Maak gebruik van die tabel om verskille tussen die fotosintetiese elektrondonors van anoksigeniese en oksigeniese fotosinterende bakterieë te verduidelik. / Make use of the table to explain differences in the photosynthetic electron donors of anoxygenic and oxygenic photosynthetic bacteria.

| | |
| |Oksigeniese |
| |fotosinterende |
|Anoksigeniese fotosinterende bakterieë / anoxygenic photosynthetic bacteria |bakterieë / Oxygenic |
| |photosynthetic bacteria |
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(12)
VRAAG 32/ QUESTION 32
Wat is die selmorfologie en Gramkleuringreaksie van elk van die volgende genera?/ What are the cellular morphology and Gram staining reaction of each of the following genera?

Vibrio ..............…………………………..…………………………………..……………….………

Clostridium ….............................……….....…………………………………………………….....

Streptococcus............................……….....………………………………………….…………(3)

AFDELING B / SECTION B

Beantwoord AL die vrae van hierdie afdeling in die eksamenboek. / Answer ALL the questions of this section in the exam book.

VRAAG 1 / QUESTION 1

Wat is die algemene eienskappe, insluitende morfologie, Gramkleuringreaksie en habitat, van die genus Streptomyces? Verduidelik ook die rol van hierdie genus in die natuur, asook in die mens se welsyn. / What are the general characteristics, including morphology, Gram staining reaction and habitat of the genus Streptomyces? Also, explain the role of this genus in nature, as well as in man’s welfare. (10)

VRAAG 2 / QUESTION 2

Verduidelik wat die pers membraan is wat by sommige halobakterieë aangetref word. / Explain what the purple membrane is that occurs in some halobacteria. (5)

VRAAG 3 / QUESTION 3

Verduidelik hoe sommige Planctomycete beide NO3- en N2 produseer. Teken ook ‘n geannoteerde skets waarin die morfologie van hierdie bakterieë verduidelik word, insluitende die setel van die energie-lewerende reaksies binne van hierdie organismes. / Explain how some Planctomycetes produce both NO3- and N2. Also, draw an annotated sketch explaining the morphology of these bacteria, including the site of the energy yielding reactions. (10)

VRAAG 4 / QUESTION 4

Teken geannoteerde sketse wat die verskille in endospoor grootte en ligging demonstreer wat gebruik word om tussen taksa binne die genera Bacillus and Clostridium te onderskei. Dui ook aan watter een van die betrokke sketse kenmerkend van die morfologie van Clostridium tetani is. / Draw annotated sketches demonstrating the differences in endospore size and location that are being used to identify different taxa within the genera Bacillus and Clostridium. Also, indicate which one of the sketches is characteristic of the morphology of Clostridium tetani. (5)

VRAAG 5 / QUESTION 5

Verduidelik kortliks deur van 'n tabel gebruik te maak hoe Bacillus anthracis en Saccharomyces cerevisiae van mekaar verskil ten opsigte van selwande, membrane, mitochondria en mitose. / Briefly explain by making use of a table how Bacillus anthracis and Saccharomyces cerevisiae differ from each other with regard to their cell walls, membranes, mitochondria and mitosis. (5)

VRAAG 6 / QUESTION 6

Bespreek die kenmerkende eienskappe van die genus Staphylococcus en verduidelik wat die impak van hierdie bakteriese genus op die mens is. / Discuss the characteristics of the genus Staphylococcus and explain the impact of this bacterial genus on man. (10)
VRAAG 7 / QUESTION 7

Hoe definieer mikrobioloë 'n fungus? Noem ook vyf van die ses hoof groepe fungi. / How do microbiologists define a fungus? Also, name five of the six major fungal groups. (10)

VRAAG 8 / QUESTION 8

Verduidelik die lewensiklus van ‘n tipiese sampioen deur gebruik te maak van geannoteerde sketse met byskrifte. Verduidellik ook die funksie van klampkonneksies in hierdie sketse. / By making use of annotated sketches, explain the life cycle of a typical mushroom. In these sketches, explain the function of clamp connections. (10)

VRAAG 9 / QUESTION 9

Hoe is dit moontlik dat Clostridium in gekookte, geblikte vleis kan oorleef en selfs binne die onoopgemaakte blikkie kan groei? U antwoord moet kenmerke van die organisme se morfologie en fisiologie aanspreek. / How is it possible that Clostridium can survive in cooked canned meat and even be able to grow in the unopened tin? Your answer should address features of the organism’s morphology and physiology. (10)

TOTAL [120]

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