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Mirco Task 2

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Microbiology Task 2
Obligate aerobes are organisms that only grow when oxygen is present. Obligate aerobes get energy from aerobic respiration. Facultative aerobes are organisms that can live with or without oxygen present. I incubated L. acidopholis and S. epidermidis at 37C. The incubation sites should stay a consistent temperature for at least 24 hours. Mesophiles are bacteria that best grow in moderate temperatures (between 25-45C). Most bacteria are mesophiles. Mesophile bacteria could include soil bacteria and bacteria that live on or within the body.
When you want to prevent the growth of microorganisms you would want to control the growth of microbes. For the transferring of organisms for this experiment I used aseptic technique which is preventing the growth of microbes. There are several methods of sterilization including heat, incineration, boiling, autoclaving, and dry heat. Heat is the most commonly used. Thirty minutes of boiling kills everything except some endospores. Autoclaving is steam under pressure and is good for sterilizing almost anything. Autoclaving kills everything, including endospores. Dry heat is only used for things that won’t melt and must remain dry. Pasteurization kills most vegetative bacterial cells and pathogens.
Types of culture media include nutrient and bacterial growth medium. Nutrient media is the standard growth media used for culturing most microbes. Nutrient media is available in both liquid broth and solidified agar. The MRS culture media has a rich nutrient base and special growth factors including polysorbate, acetate, magnesium, and manganese (Alonzo, 2010). L. acidopholis is cultured using the MRS media because it will not grow in the nutrient media. The nutrient media culture is used with the S. epidermidis.
I did not see growth after 24 hours. I started seeing growth around 45 hours. I

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