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Mlt 1 Project

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Submitted By ldavi63
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MLT1 Project
Lisa Davidson
Western Governors University
May 17, 2015
MLT1 Project
A. Difference Between an Obligate and Facultative Aerobe
An obligate aerobe has to have oxygen to grow, while a facultative aerobe can survive with oxygen present or no oxygen present (Todar, 2012).
B. Temperature Requirements and Types of Organisms
The temperature requirement for this experiment is twenty-four degrees Celsius or above which is room temperature. The two types of organisms cultured are lactobacillus acidophilus and staphylococcus epidermidis. Lactobacillus acidophilus is a probiotic which is a live bacteria that is good for your health and found naturally in foods like yogurt. Staphylococcus epidermidis is a gram positive organism which is found naturally on our skin.
C. Conditions Necessary to Control Microbe Growth
In this experiment, microbe growth was controlled by providing access to oxygen, which was achieved by loosening the tops on the test tubes, and maintaining a constant temperature of twenty-four degree celsius or above. Also needed for the lactobacillus acidophilus to grow, was nine milliliters of MRS broth media. In order for the staphylococcus epidermidis to grow, five milliliters of nutrient broth was needed to house a cotton swab that was moistened and rubbed on skin. Microbial growth is not bad if it is a good microbe; however, certain microbes cause disease. This growth left uncontrolled can cause individuals to become sick or even die. To control growth means to prevent growth or inhibit growth. Cidal agents are chemical agents that can kill microbes, and static agents are chemical agents that inhibit microbial growth (Todar, 2012).
D. Basic Forms of Culture Media
There are six basic forms of culture media:
1. Basal media: This media can be used for growth of bacteria not dependent on media environment.
2. Enriched media:

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