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Molecular Genetics

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GENETIKA 244

INLEIDENDE MOLEKULÊRE GENETIKA
Tutoriaal 1: DNA struktuur en replisering
Vraag 1 a. Noem die funksies wat aan genetiese materiaal toegeskryf word en verduidelik wat elkeen van hierdie terme beteken. [6] b. Noem vier eienskappe van DNA soos vervat in die Watson & Crick model [4] c. Noem die twee klasse stikstofbasisse wat voorkom, asook die die basisse wat in elke klas voorkom. [2] d. Teken die chemiese struktuur van die drie komponente van ‘n nukleotied en las hulle dan aanmekaar. Watter molekule word vrygestel as die drie aanmekaar skakel? [4] e. Noem vier verskillende metodes of tegnieke wat vir die analise van nukleïensure gebruik word. [4] f. Beskryf die bydraes wat Griffith, en Avery et al. gemaak het tot die ontrafeling van genetiese transformasie en let veral op die verskille. [4]

Vraag 2 a. Vergelyk die konserwatiewe, semi-konserwatiewe en dispersiewe modelle van DNA replisering [6] b. Maak ‘n vergelykende opsomming van die eienskappe van DNA polimerases I, II en III. [6] c. Watter gebeurtenis het bewys dat DNA polimerase I nie die enigste ensiem is wat betrokke is by DNA sintese nie. [1] d. Wat word die kompleks van proteïene genoem wat betrokke is by DNA replisering? [1] e. Die genoom van die vrugtevlieg beslaan ongeveer 1.6 x 10 basispare. DNA sintese gekied teen 30 basispare per sekonde; en in die vroeë embrio word die hele genoom in 5 minute ge-repliseer. Hoeveel twee-rigting (bidirectional) oorspronge van sintese word benodig om dit reg te kry? [2]
8

Vraag 3 a. In die meegaande diagram van DNA replisering van E. coli verwys die pyle na die spesifieke genommerde vrae. Beantwoord al die vrae. [8]

1. Watter punt (5’ of 3’) v/d molekule?

2. Ensiem betrokke by verligting van supercoiling. 3. Ensiem betrokke by afraai van DNA heliks 4. Watter nukleïensuur en watter strukture? 5. Wat word h/d kort fragmente genoem? 6. Ensiem wat

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