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Monosaccharide Lab Report

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The Role of Polysaccharides and Monosaccharides in Metabolism and Their Rate of Carbon Dioxide Produced During Fermentation

Abstract
Fermentation is the metabolization of sugars with carbon dioxide being produced as a result. Using yeast to ferment different types of sugars, we tested the rate of carbon dioxide production. Our findings of sucralose producing a slower rate of carbon dioxide than glucose during fermentation, are supported by the fact that polysaccharides are harder to break down than monosaccharides. Polysaccharides are complex sugars, or chains of monosaccharides, so they are harder and take longer to be broken down and metabolized.

Introduction
Glycemic Index is described by how fast your body converts the carbohydrates …show more content…
Our control was the glucose solution which is a monosaccharide, and the experimental group was the sucralose solution which is a polysaccharide. To test our hypothesis, we first connected a carbon dioxide probe to a lab quest mini and connected it to out lap top that had logger pro software running. This probe needs to be connected for at least 5 minutes before any testing can be done. We then collected 5 ml of 1.5 mole sucralose and glucose in separate graduated cylinders. We also collected 10 ml of 62.5 mg/ml yeast and placed 5ml in two separate graduated cylinders. Once the 5 minutes was over we placed a respiratory chamber containing the magnet on a temperature stir station. After that, we poured our yeast in to the station and turned on the stir station to setting one and quickly poured in the glucose and placed the carbon dioxide probe inside, while simultaneously starting our trial on the logger pro software. We then waited exactly 4 minutes, while recording data every 10 seconds and logged the amount of CO2 in parts per million. We then repeated this process for the sucralose solution. We tested each solution 2 times in order to collect accurate data. We analyzed the data using a single factor …show more content…
“Glycolysis.” Khan Academy, www.khanacademy.org/science/biology/cellular-respiration-and-fermentation/glycolysis/a/glycolysis. deFigueiredo, Eduardo Barreto, et al. "Greenhouse Gas Emission Associated with Sugar Production in Southern Brazil." Carbon Balance & Management, vol. 5, Jan. 2010, pp. 3-9. EBSCOhost, doi:10.1186/1750-0680-5-3.
Tu, Benjamin P., et al. "Logic of the Yeast Metabolic Cycle: Temporal Compartmentalization of Cellular Processes." Science, vol. 310, no. 5751, 18 Nov. 2005, pp. 1152-1158. EBSCOhost, libraries.ou.edu/access.aspx?url=http://search.ebscohost.com/login.aspx?direct=true&db=aph&AN=19014237&site=ehost-live..
Angustia, Jason, et al. “The Effect of Different Sugars in the Medium on Carbon Dioxide Production in Saccharomyces Cerevisiae .”

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