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Mr. Condella

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Submitted By endurancemike
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General Biology I & II - BIO195/BIO196
Laboratory Report Cover Sheet

Abstract (10pts)
Observations/Data (20pts)
Answers to Questions (40pts)
Overall effort (30pts)

Michael Condella
Name: ________________________________________________

Matt, Pye
Partner’s name: ______________________________________
02/04/16
Date Completed: _________________

BIO195

BIO196

Total score

02/11/16
Date submitted: _________________

T1
Course section: ________

Introduction to Gel Electrophoresis
Investigation Title: _________________________________________________

ABSTRACT
Your abstract must include the major objective(s) for this investigation along with a summary of what you learned or concluded from your results. Your abstract should be in paragraph form.
Gel Electrophoresis is a process by which macromolecules like DNA are separated based on their size. DNA is carried with electricity in an apparatus across an agarose gel matrix. The gel slab is burrowed in a conductive solution, allowing DNA to be measured for analytical and diagnostic purposes.
We analyzed DNA from a crime scene and compared it to DNA from five suspects. We trimmed each suspects DNA with restrictive enzyme and then pulsed the tubes in the centrifuge that were then incubated. We then deployed micro amounts of DNA from each suspect into dedicated lanes on the agarose gel slab via micropipets. We introduced an electrical field, the current caused the negatively charged DNA to migrate to the anode pole of the apparatus. While the DNA was migrating to the anode pole, the agarose gel acted like a selectively permeable membrane, giving way to a plurality of migrations for each suspect, inversely proportional to each suspects nucleotide allotment. It came as no surprise that smaller DNA fragments traveled a greater net distance than DNA fragments that were more robust in size.
Gel Electrophoresis is efficacious in law enforcement because it exploits an individuals unique
RFLP. It's extremely improbable that two individuals will have the same RFLP. My partners and
I were able to deduce that suspect 3 matched the DNA from the crime scene almost perfectly, proving beyond a shadow of a doubt that suspect 3's DNA was at the crime scene. We discovered Gel Electrophoresis is also useful in medical application. Serum Protein
Electrophoresis (SPEP) measures proteins in the blood to help diagnose disease.

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