To start the lab each student was required to have on a pair of goggles, long pants, and an apron on at all times. First, start by receiving four sterile nutrient agar plates then writing on each perimeter of the agar plates, with a sharpie, the student's initials, instructor name, class period, and the product being tested on the bottom of the agar plate. Be sure that the bottom of the agar plate stays facing up. Next, get two sterile cotton swabs and dip the tips of each swab into the culture tubes. Be very careful when using the cotton swabs, do not let the tips touch any human skin. After dipping the cotton swab, lightly rub the swab in a horizontal zig-zag pattern then a vertical zig-zag pattern. Repeat the same pattern on each agar plate and use a different bacteria-infected swab for each agar plate. …show more content…
Using the correct forceps, pick up one paper disk from the distilled water petri dish and place it in the center of the corresponding agar plate. Repeat this process 3 more times but use the household products being tested and the correctly labeled petri dish. Make sure that after this step the Petri dishes are closed. A good technique for putting the parafilm on for the next step would be to place a part of the parafilm on the lid of the petri dish and place your finger on it. Then, stretch the parafilm out using your other hand and wrap it around as much as it can around the dish. The lid of the petri dish should not be able to come off after this step. The Petri dishes will then have an incubation time of 48 hours at 23 degrees Celsius, they are stored upside down to prevent the bacteria from getting wet. The cleaning process is simple, after rubbing the cotton swabs on each agar plate, place them in an ammonia solution. Throw away the wrappers of the cotton swabs. Place the forceps above there corresponding products petri