...The purpose of this lab was to genetically transform bacterial plasmid DNA with foreign pieces of DNA, pGLO, that causes expression of an ampicillin resistant gene, and see the effects it has on bacterial growth in presence of the antibiotic, ampicillin. We inserted pGLO DNA into the genome of the bacteria through the use of inoculation loops, Laurel Broth, transformation solution, and procedures such as heat shock and incubation. This experiment involved four bacterial agar plates, two of which we genetically transformed (experimental groups; +pGLO LB/amp/ara and +pGLO LB/amp), and two of which we did not genetically transform and were control plates (-pGLO LB and -pGLO LB/amp/). Out of the two genetically transformed bacteria, only the bacteria containing arabinose sugar (+pGLO LB/amp/ara) initiated expression of the GFP (Green Fluorescent Protein) gene, causing the bacterial...
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...The materials used in the transformation of Escherichia Coli include a transformation solution, a starter plate of bacteria colonies, and pGLO. A bacterial suspension is created by transferring 250 μl of transformation into two sterile 2.0 microcentrifuge tubes of different colors. One of the tubes is labeled +pGLO and the other is labeled –pGLO. Once 250 μl transformation solution is in each of the tubes they are placed on a round floating tube rack and then placed on ice. To complete the bacterial suspension, 2 large or 3 small colonies of bacteria are taken from the starter plate using a sterile loop. Holding the +pGLO tube between two fingers, the loop with the bacterial colonies is placed in the transformation solution. To ensure that the entire colony is completely dispersed in the transformation solution the loop is spun vigorously until there are no floating chunks remaining in the solution. Using a new sterile loop, this same process is...
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...London School of Engineering and Materials Science Laboratory report writing instructions DEN101 - Fluid Mechanics 1 Flow Rate Measurement Experiment A. Student Student Number: 1234567 Version 2.0, 27 November 2010 Template for Word 97-2003 Abstract This document explains what is expected in your Fluids 1 lab report. The sections that should be covered are outlined and a structure you could follow is proposed. Detailed advice on how to edit the report is given. The document concludes with the marking criteria for this lab report. Table of Contents Abstract 2 1. Introduction 3 1.1. Writing 3 1.2. Editing and formatting 3 1.3. Content of the introduction 4 2. Background and theory 4 3. Apparatus 4 4. Test 4 5. Experimental procedure 4 6. Results 5 7. Discussion 5 8. Conclusions 5 9. References 5 10. Appendix A: Marking criteria 6 Introduction Before starting to write a report, you should think about what is your audience. Am I writing for colleagues who want a lot of detail how it is done, or am I writing for my boss who just wants an executive summary as he has no time for details? In general, there is not a single type of audience and we have to make our writing suitable for the detailed read, as well as the fast perusal. To understand what is required from you in this report, please have a look at the marking criteria in the Appendix. 1 Writing To limit...
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...Biology Formal Lab Report 2 Intro Throughout this lab, the main topic is genetic transformation. Genetic Transformation is a phenotypic change caused by genotype change from transferring foreign genes into an organism. This lab is to transform the bacteria E.Coli with the GFP gene (Green Fluorescent Protein). GFP makes organisms glow in the dark with UV light, similar to jellyfishes.GFP will be transferred into E.Coli through the use of recombinant DNA, or simply a vector. A vector is a plasmid that transfers foreign DNA into cells. Bacteria can transfer plasmid to other bacteria so they can survive and adapt to the environment they are in. The pGLO plasmid includes the GFP gene (allows bacteria to glow), Beta-lactamase (resists ampicillin), ORI sites (allows bacteria to self replicate), and AraC (allows induction of GFP gene). Materials and Methods: In this lab, first there will be two micro test tubes, one labled pGLO+ and the other pGLO-. Using a sterile transfer pipet, transfer 205 microliters of CaCl2 into each tube. Then put the tubes on ice. Then use a sterile loop to pick up a single colony of bacteria from the starter plate. Put the colony in the pGLO+ tube and spin the loop. Then put the tube back in ice. Repeat this for the pGLO- tube with a new sterile loop. Next use a new sterile loop and get some DNA stock. With the loop, immerse it in only in the pGLO+ tube. After that, incubate the tubes in ice for 10 minutes. Label one plate LB/amp +pGLO, another LB/amp/ara...
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