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Ph 13.0 Lab Report

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1.Polusine at pH 13.0 and polyglutamic acid at pH 1.5 will form an alpha helix in solution at room temperature because the group of amino acids have to be either acid or base in order to become protonated or deprotonated in the environment. Polyglycine, polyisolecucine, and polyaspartic acid at pH 7.0, the amino acid is neutral condition, cannot protonated or deprotonated.
2. see attach page
3.
a) The optimum pH is where the most active of enzyme. The profile shows that the optimum pH is 8.0. The optimum pH 8.0 is the amino acid that contain R group with proper charge. In the enzyme activity, the pH too low or too high will affect the enzyme to denaturation, affect the subtract bind to the active site. There is only one specific substrate

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