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Photosynthesis Lab

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Abstract: The purpose of this lab is to measure the rate of photosynthesis in isolated chloroplasts. The lab begins with adding solutions to multiple cuvettes, then adding either boiled chloroplasts, not boiled chloroplasts or no chloroplasts. After approximately 5 minute timed sessions in light, tubes are placed in spectrophotometer one at a time and the transmittance percentage is recorded for each test. Boiled chloroplasts are denatured, so photosynthesis cannot occur. Solutions in test tubes with not boiled chloroplasts become clear after light reaction. Light cannot reach to the not boiled chloroplasts in foil test so a very minimum change occurs. When the transmittance percentage increased, photosynthesis had occurred.
Introduction:
Photosynthesis converts solar energy from sunlight into the chemical energy that is used to fuel the organism’s activities. The process of photosynthesis can be summarized by the following equation: solar energy + H₂O + CO₂ ---> C₆H₁₂O₆ + O₂ …show more content…
The chloroplast contains two membranes; the inner and outer membrane. Within the chloroplasts, there is a membrane system called thylakoids; which are interconnected disks that look flattened. The thylakoids are stacked to form a granum (plural is grana). Chlorophyll’s are molecules that stay on the surface of the thylakoids and capture light energy from the sun. Thylakoids are a crucial part to photosynthesis because once the solar energy is absorbed, the energy is embedded in the thylakoid membrane. Stroma is an aqueous fluid that surrounds the grana, which contains a rich mixture of enzymes and metabolic

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