An experimental method for determining pyrantel pamoate (PYR) via an isocratic reversed phase HPLC method with UV detection was previously optimized by Solich et al. In our experiment, we determine the PYR concentration in an old sample of a novel, anthelmintic raccoon bait. Chromatography was performed using a phenyl-hexyl column with a mobile phase of 0.5% triethylamine at pH 9.0 and acetonitrile 55 : 45 (v/v) at a flow rate of 1.0 ml min-1. For pyrantel determination, UV detection was performed at 290 nm. Analysis of the chromatographic study of the pyrantel base peaks showed that PYR makes up 2.05% of the raccoon bait by weight.
Introduction
Pyrantel pamoate is widely used in a variety of anthelmintic drugs, in the pharmaceutical and veterinary industries, that are designed to fight parasitic worm infections in both humans and animals. Raccoons …show more content…
Throughout the course of the experiment, the retention times and peak heights for both our standards and our sample fluctuated considerably, especially after the HPLC needed to be degassed. Due to these inconsistencies, we have chosen to stick with our data from before the system went offline, which itself was much more consistent, to report our data. The mass percent of PYR present in the raccoon bait sample was determined to be 2.05%. To confirm that the sample peak we were observing was, in fact, PYR we spiked our sample with Standard 2 for a resulting solution that was Standard 2-sample 10 : 90 (v/v). The spike qualitatively confirmed the presence of PYR in the sample, as the resulting peak showed a decrease in peak height from Standard 2 and an increase in height from the sample. A quantitative assessment was not possible, because our data for the sample, taken on the day we ran the spike, was not consistent with previously recorded