...PRIMER DESIGN QUIZZ Basing on the properties of primer for PCR process, a primers are designed to satisfy most of the requirements to be a good primer: The forward primer: 5’- VR THGAGTWYTA YRTYRTVG - 3’, the template from nucleotide 399 to 418 The revere primer: 3’- G TGAARTYVCG HACCSKN - 5’, the template from nucleotide 609 - 627 1) The reason for the region chosen: There are two basic reason for the region chosen for primer design: - Firstly, in this region, there are a high similarities of the DNA sequence of 4 organisms. - Secondly, the percentage of G and C are satisfied in the range of 40 - 60%. 2) The forward primer is VR THGAGTWYTA YRTYRTVG and have the direction of 5’ end to 3’ end: 5’- VR THGAGTWYTA YRTYRTVG - 3’ The reverse primer has an opposite direction which is 3’- G TGAARTYVCG HACCSKN - 5’ 3) The ideal length of PCR primer for general application should be between 18 to 24 bp (Dieffenbach, Lowe, & Dveksler, 1993). In reality, if the primer is too short, it will possess low specificity result in non-specific amplification. However, in another case that it is too long, it will decrease the template-binding efficiency at normal annealing temperature due to the high potential of forming secondary structure such as hairpins. The designed primer has the length of 20 bp with forward primer and 18 bp with reverse primer. This length is in the optimal condition for PCR primer. 4) The primers are always specified 5’ to 3’, and in the PCR...
Words: 882 - Pages: 4
...Instructions: 1. This question paper consists of 2 sections A and B. 2. Answer all the questions. 3. Mobile phone and any other Mobile electronic devices are NOT allowed into the examination room. SECTION A 1. ____ is an important component of every information system that helps organizations to achieve their goals. a. | Hardware | c. | Feedback mechanism | b. | Software | d. | Data | 2. The value of information is directly linked to how it helps decision makers achieve their organization’s ____. a. | profits | c. | cost reduction initiatives | b. | goals | d. | quality improvement measures | 3. In information systems, ____ is used to make changes to input or processing activities. a. | forecasting | c. | output | b. | feedback | d. | processing | 4. ____ is (are) considered to be the most important element in a computer-based information system. a. | Hardware | c. | Procedures | b. | Software | d. | People | 5. People who work with information systems to get results are called ____. a. | the CIO | c. | IS personnel | b. | users | d. | IS managers | 6. A common type of information system used in business organizations are those designed for ____. a. | electronic and mobile commerce | c. | decision support | b. | transaction processing | d. | all of the above | 7. ____ involves using information systems and the Internet to acquire parts and supplies. a. | e-commerce | c. | mobile commerce...
Words: 1781 - Pages: 8
...SATURDAY SUNDAY 27th of August 15:0017:00 First Lecture Chapters 1 and 2 Introduction to the Course & the World of Retailing B1033 28 of Aug. Chapters 1, 2, 3 Introduction 10:0012:00 B4065 30th of August 15:00-17:00 Second Lecture Chapters 6 and 14 Legal & Ethical Aspects, and HRM B1033 SEPTEMBER MONDAY 2nd of Sept. Chapters 4 and 7 Perception, Learning and Memory 14:00-16:00 B4065 TUESDAY WEDNSDAY 4th of Sept. Chapters 5 and 6 The Self, Personality and Lifestyle Motivation and Values 14:00-16:00 B4065 THURSDAY FRIDAY 6th of September 15:0017:00 QUIZZ 1 Lecture 3 Chapters 3 and 4 Retail Customers & Competition B1033 13th of Septrember 15:0017:00 Lecture 4 Chapter 5 Retail Supply Chain Management B1033 Opportunity to discuss our project in Consumer behaviour 10:00-12:00 B2021 SATURDAY SUNDAY 11th of Sept. What consumer research is realy all about 11:0013:00 B4065 17th of Sept. QUIZZ 2 Lecture 5 14:00 -16:00 Chapters 7 & 13 Retail Location Selection and Opportunity to Store Layout Guest discuss our project Lecture 16:00-17:00 B1033 10:00-12:00 B2021 Opportunity to discuss our project in Consumer behaviour 10:00-12:00 B2021 23rd of Sept. Decision making, Buying and disposing Chapter 9 12:0014:00 B4065 23rd of Sept. Guest Lecture 13:00-15:00 B1033 MONDAY Opportunity to discuss 20th of Sept. Chapter 8 our project 10:00Attitudes 11:00-13:00 B4065 12:00 B2021 24th of Sept. Seminar 10:00-12:00 10 min. per Group B4045 25th of Sept. Chapters 10...
Words: 423 - Pages: 2
...UNIT 1 V1. Introduction: The EU in the New World Order (3 Videos) Questions: 1. Who has the power to represent the EU internationally? The president of the European Commission, the high Representative of the Union for Foreign Affairs and Security Policy, the head of delegation of the EU to third countries (e.g. the US),and the president of the European Council. 2. Of which of the following international organisations is the EU a (full) member? The FAO and WTO, two conditions need to be fulfilled: 1) The EU must have the power (as conferred by the Member States in the Treaties) to become a member, 2) the international organization must allow the EU (which is not a state) to become a member. Consult, for instance, Art. 4 of the UN Charter 3. What is the EU in international law? Rights and obligations. It is not a state or is a regional integration organization with a very extensive measeur of international legal personality (at 47 TEU) 4. Who represents Europe in the world, in external affairs and treaty negotiations with 3rd countries or international organizations, High representative of EU for foreign affairs and policy: created by the Lisbon treaty , it is also the vicepresident to the European Comissions. Head of state, Europe is represented by 2 persons: permanent president of European Council and President of the European Commission (more for classical competences of the EUUU). And Rotatic council. World wide diplomatic network of the European Union:...
Words: 9149 - Pages: 37
...Student corner n°1 Drinking and driving The text October 18, 2011, The NY times Friends Still Let Friends Drive Drunk By BARRON H. LERNER, M.D. On Sept. 15, Matthew Grape, 21, got into the passenger seat of a car with one of his Duke University fraternity brothers. The driver hit a tree, escaping with minor injuries, but killing his dear friend. According to local press reports, the driver was charged with impaired driving. Nearly 11,000 deaths related to alcohol-impaired driving still occur each year in the United States, despite a three-decade surge in anti-drunken driving activism, stricter laws and clever slogans like “Friends don’t let friends drive drunk.” There are more than 110 million instances of impaired driving each year, according to data from the 2010 Behavioral Risk Factor Surveillance System Survey and summarized in a new report from the Centers for Disease Control and Prevention. How can this still be occurring, and what can be done about it? Back in 1980, when Candy Lightner’s 13-year-old daughter, Cari, was killed in California by a drunken driver with several previous arrests for driving while intoxicated, including one only two days earlier, there was little public discussion of the topic, even though drunken drivers killed 25,000 Americans annually. Crashes were called “accidents,” and victims were characterized as having been “in the wrong place at the wrong time.” Police and prosecutors routinely allowed drunken drivers to plead to lesser offenses...
Words: 1890 - Pages: 8
...tions Managmen Introduction What makes a good process? When a process operates, how do you determine whether it is successful or not? Processes seem simple, yet seem to cause so many problems and the very word 'process' can strike fear into the hearts of many people. If you are leading any type of process improvement program, you have probably felt at times like your efforts were yielding less-than-expected results. It is no wonder: the list of possible hurdles to overcome – restrictive organizational structure, politics, stagnant organizational culture, power trips – goes on and on. Continuous Improvement is the on-going effort to improve products, services and processes by making small, incremental improvements within a business. It is based on the belief that these incremental changes will add up to major improvements over time and it is as much about tactics (i.e. specific improvements) as it is about changing the culture of the organization to focus on opportunities for improvement rather than problems. At the other extreme, there is the Business Process Reengineering which advocates starting from a clean slate with a quantum leaps. Whatever techniques/methods is used it relative the pulse of an organisation. Your objectives In this chapter you will learn about the following: * Understand the 3 Es of operational excellence * Understand the importance of the 3 Es * Understand the techniques/methods to achieve the 3 Es * Understand how to acehive...
Words: 5180 - Pages: 21
...Intl Project Management Cost & finance Part 2 : Tools and techniques SupdeCo La Rochelle (H. Khatchadourian) Aligned with the PMBOK® Guide Fourth Edition 1 Investment Concept & project investment Investment : basics The process of compounding and discounting Compound interest and future value (FV) of a single payment (« lump sums ») FVn = PV(1+I)n With N number of period (= years, quarters,months) I interest rate PV present value FV future value at end of year « n » Step-by-step approach or formula approach Use of spreadsheets (Excel) 1. Int. 2. Scope 3. Time 4. Cost 5. Quality 6. HR 7. Com. 8. Risk 9. Proc. Investment: basics Present value (PV) PV = FVn / (1+I)n Step-by-step approach and formula approach Finding the interest rate « i » ? Finding the number of years « n » ? 1. Int. 2. Scope 3. Time 4. Cost 5. Quality 6. HR 7. Com. 8. Risk 9. Proc. Investment: basics Annuities (ordinary) A series of payments over time At the end of each period Periods 0 5% 1 2 3 Payments -100€ -100€ -100€ 1. Int. 2. Scope 3. Time 4. Cost 5. Quality 6. HR 7. Com. 8. Risk 9. Proc. Annuities (due) At the beginning of each period Investment: basics Future value of an ordinary annuity With ⎡ (1 + I )n − 1⎤ FVAn = PMT ⎢ ⎥ I ⎣ ⎦ Interest rate I Series of payment (= constant payment) 1. Int. 2. Scope 3. Time 4. Cost 5. Quality 6. HR 7. Com. 8. Risk 9. Proc. Investment: basics Future value of an ordinary annuity Periods 0 r=5%...
Words: 10588 - Pages: 43
...Marketing Management MKTG 5150, 007, 077, 080, 086 Fall 2013 8W Instructor: Kenneth N. Thompson, Ph.D. Professor of Marketing & Logistics Office Hours: 1:30 – 3:30 TTh and by appointment E-‐mail: kenneth.thompson@unt.edu. Only use this e-‐mail address if you cannot reach me via the Blackboard Learn e-‐mail facility. Use the ‘e-‐mail’ selection on the Learn menu bar (left side of the Learn window). E-‐mail traffic initiated via Blackboard Learn is specifically flagged to get my attention. By the way, do not expect me to respond to e-‐mail on weekends or after 5:00 p.m. on weeknights. I am often online during these times, but there are no guarantees with my extensive travel schedule this Summer. Roger Kerin and Robert Peterson (2010), Strategic Marketing Problems: Cases and Comments, 12th Ed. (Upper Saddle River, NJ: Pearson Prentice Hall) ISBN-‐13: 978-‐0-‐13-‐...
Words: 6865 - Pages: 28
...1 Challenges We Face in FM by John Vinken, IFMA Toronto President Vinken’s Views - President’s Message Spring has sprung in Southern Ontario and my mind races onward with ideas that I want to implement both at work and home. And what a better way to explore these ideas and develop some structure around them, than by hiring a person that is motivated, energetic, and able to acquire current knowledge easily. Yeah I know you are asking “Where do I get in line for someone like that?” Well what I have just described is a co-op student. I have my usual 2 co-op students already reserved and they will be starting in 3 weeks time. And let me tell you my whiteboard is filling with initiatives and ideas for these two to deal with. And with two facility management related college programs in the area (at Humber College and at Conestoga Collage) there are co-op students looking for jobs in the facility management field. If you want the contact information use the membership CD just sent to you and on the opening page are the hyperlinks for both of these institutions. This is the third year that IFMA Toronto has produced the membership CD rather than published a membership roster. There are, of course, issues with this year’s CD as there have been with every membership roster created in history I believe. Some of the problems are related to getting updated information about member. As an example when people renew their membership and if it is tardy the membership listing we acquire...
Words: 7699 - Pages: 31
...Mémoire de recherche Master 1 _______________________________ Les réseaux sociaux et l’évolution des usages Année 2011 Tutrice : Hélène Rodriguez Alexandre De Munter Aurélien Menu Résumé : Nous avons choisi de réaliser notre mémoire sur le thème des réseaux sociaux, leurs évolutions et leurs usages. Afin de ne pas seulement vous définir ce que sont les réseaux sociaux, nous avons décidé de combiner ce thème avec le marketing. Nous sommes tous les deux, présents sur les réseaux sociaux, nous avons grandit avec et nous vivons au quotidien avec. Nous voulons également par la suite, nous orienter vers le marketing, il était donc presque logique d’axer notre mémoire de recherche sur le marketing, et grâce à notre problématique, nous avons pu travailler sur les deux. La thématique de ce mémoire de recherche permet de voir non seulement ce que les entreprises ont déjà pu faire en terme de marketing et de réseaux sociaux, mais également les opportunités et les menaces que les réseaux sociaux représentent pour les entreprises. Abstract : We chose to make our memory of search on the topic of social networks, their evolution and their uses. In order not only do you define what social networks, we decided to combine this theme with marketing. We are both present on social networks, we grew up with and live with everyday. We also want to work in marketing after ours studies, so it was almost logical to focus our research paper on marketing. And thanks to our problem, we...
Words: 17647 - Pages: 71
...Le CNC et QualiQuanti remercient les experts qui ont apporté leurs conseils tout au long de cette étude, avec une mention particulière à la société Wiztivi, qui les a accompagné et leur a permis d’utiliser son show-room pour les tests utilisateurs. Cette étude a été réalisée par : Daniel Bô, Alexandre Cavallero, Claire-Marie Lévêque, Raphaël Lellouche En partenariat avec : Eric Bibollet Centre national du cinéma et de l’image animée Direction des études, des statistiques et de la prospective 12, rue de Lübeck 75784 Paris cedex 16 Tél : 01.44.34.38.26 Fax : 01.44.34.34.55 www.cnc.fr Benoît Danard, Sophie Daubard, Clément Malherbe Sommaire Sommaire ....................................................................................................... 1 Objectifs ......................................................................................................... 3 Méthodologie.................................................................................................. 4 Synthèse ........................................................................................................ 5 I. LE PAYSAGE COMPLEXE DE LA TELEVISION CONNECTEE ............... 8 A. Le concept de télévision connectée et son appellation ........................... 8 B. Les usages connectés de la télévision du salon ..................................... 9 C. La télévision connectée : usage collectif ou individuel ? ....................... 10 D. La concurrence fournisseurs d’accès à...
Words: 29706 - Pages: 119
...This page intentionally left blank This page intentionally left blank Less managing. More teaching. Greater learning. INSTRUCTORS... Would you like your students to show up for class more prepared? class is much more fun if everyone is engaged and prepared…) (Let’s face it, Want ready-made application-level interactive assignments, student progress reporting, and auto-assignment grading? (Less time grading means more time teaching…) Want an instant view of student or class performance relative to learning objectives? (No more wondering if students understand…) Need to collect data and generate reports required for administration or accreditation? (Say goodbye to manually tracking student learning outcomes…) Want to record and post your lectures for students to view online? With McGraw-Hill's Connect Management, ™ INSTRUCTORS GET: • Interactive Applications – book-specific interactive assignments that require students to APPLY what they’ve learned. • Simple assignment management, allowing you to spend more time teaching. • Auto-graded assignments, quizzes, and tests. • Detailed Visual Reporting where student and section results can be viewed and analyzed. • Sophisticated online testing capability. • A filtering and reporting function that allows you to easily assign and report on materials that are correlated to accreditation standards, learning outcomes, and Bloom’s taxonomy. • An easy-to-use lecture capture tool. STUDENTS... Want an online, searchable...
Words: 209749 - Pages: 839
...This page intentionally left blank This page intentionally left blank Less managing. More teaching. Greater learning. INSTRUCTORS... Would you like your students to show up for class more prepared? class is much more fun if everyone is engaged and prepared…) (Let’s face it, Want ready-made application-level interactive assignments, student progress reporting, and auto-assignment grading? (Less time grading means more time teaching…) Want an instant view of student or class performance relative to learning objectives? (No more wondering if students understand…) Need to collect data and generate reports required for administration or accreditation? (Say goodbye to manually tracking student learning outcomes…) Want to record and post your lectures for students to view online? With McGraw-Hill's Connect Management, ™ INSTRUCTORS GET: • Interactive Applications – book-specific interactive assignments that require students to APPLY what they’ve learned. • Simple assignment management, allowing you to spend more time teaching. • Auto-graded assignments, quizzes, and tests. • Detailed Visual Reporting where student and section results can be viewed and analyzed. • Sophisticated online testing capability. • A filtering and reporting function that allows you to easily assign and report on materials that are correlated to accreditation standards, learning outcomes, and Bloom’s taxonomy. • An easy-to-use lecture capture tool. STUDENTS... Want an online, searchable...
Words: 211687 - Pages: 847