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Red Tilapia Lab Report

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Red tilapia is gaining popularity nowadays in the market but only limited studies on its behavioral and physiological response to stress are available. In this study, 27 red Nile tilapia (RNT) were subjected to confinement and social stresses. This experiment monitored their physiological (plasma cortisol) and morphological [ventilation rate (VR) and skin color] responses to stress. The plasma cortisol and VR of the fish before exposure to stressors were significantly lower compared to there values after the introduction of stressor. For fish subjected to social stress, there was also a significantly higher cortisol level and VR in the dominant fish compared to those in the subordinate fish. The skin color of red tilapia was also …show more content…
For the confinement experiment, nine RNT weighing 45-100 grams were randomly selected and used. On the other hand, 18 RNT were also used for social experiment. Dominant fish which weighed 50.0-90.6 grams were used as social stressors and were selected to be heavier than the subordinate fish which weighed 45-81.2 grams. Isolation was imposed 10 days before the test. The fish were fed daily except during blood collection days. In each experiment, physiological (plasma cortisol) and morphological [ventilation rate (VR) and skin color] changes were monitored. The pre-stressor measurement of plasma cortisol was performed 5 days after the beginning of isolation. Five days later, the nine fish in the confinement stress experiment were divided in three groups and the three fish in each group were exposed individually to confinement stressors for 60 minutes (T60) before blood collection, or 90 minutes (T90) before blood collection or were only observed for their physical changes during stress period and there was no blood collection. The morphological changes (VR and skin color) were monitored 4 minutes and 2 minutes before introduction of stressors (pre-stress); immediately after introduction of stressor and 2, 4, 6, 8, 10, 30, 60 and 90 minutes later. In the social stress experiment, the same procedure was employed but the fish were exposed to social

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