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Sodium Difficile Research Paper

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Clostridium difficile

There are millions of different varieties of bacteria currently living in your body that cause no harm called your normal flora. These bacteria prevent infection from other bacteria that do not belong there through means of competition. Clostridium difficile is an infectious sporulating bacteria that develops your gut, causing severe abdominal pains and frequent diarrhea. This microorganism was initially isolated from a newborn’s fecal matter in 1935 by Hall and O’Toole, but the studies weren't published until 19783(Heinlen, L.2010). Bacteria constantly have to go through adaptations to their environment, find other means to live if it currently doesn't fulfill their needs for expansion or growth. …show more content…
2010). Test like PCR and sequencing were preformed to determine what mutations had occurred for this resistance to happen. The results found were mutations occurring in the active site in the DNA causing a mutation in gyrA gene effecting the resistance. Not only do mutations, multiple strains, and resistance make it difficult to control the bacteria but also the effect it has on the healthcare system. In humans C.difficile in one year alone effected half a million people in the United States. Annually anywhere from 15,000 to 20,000 people die from this infection3(Heinlen, L.2010). Studies are still currently being conducted on a approximate number of how much C.difficile infection has cost the United States healthcare system, but in 2008 it was shown through studies that it might have cost as much as $4.8 billion dollars just in acute-care facilities2(Dubberke, E. R. …show more content…
Heinlen, L., and Ballard, J. D. 2010. Clostridium difficile Infection. The American Journal of the Medical Sciences, 340(3), 247–252.

4. Hunt, J. J., and Ballard, J. D. 2013. Variations in Virulence and Molecular Biology among Emerging Strains of Clostridium difficile. Microbiology and Molecular Biology Reviews : MMBR, 77(4), 567–581.

5. Karasawa T, Ikoma S, Yamakawa K, Nakamura S. Microbiology.A defined growth medium for Clostridium difficile 141(2):371-375

6. Knight, D.R., Elliott B., Chang B.J., Perkins T.T., Riley T.V. 2015. Diversity and Evolution in the Genome of Clostridium difficile.28(3):721-41

7. Solomon, K. 2013. The host immune response to Clostridium difficile infection. Therapeutic Advances in Infectious Disease, 1(1), 19–35.

8. Spigaglia, P., Barbanti, F., Dionisi, A. M., and Mastrantonio, P. 2010. Clostridium difficile Isolates Resistant to Fluoroquinolones in Italy: Emergence of PCR Ribotype 018 . Journal of Clinical Microbiology, 48(8), 2892–2896.

9. Voth, D. E., and Ballard, J. D. 2005. Clostridium difficile Toxins: Mechanism of Action and Role in Disease. Clinical Microbiology Reviews, 18(2),

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