Solid-lipid nanoparticles (SLNs) have recently gained significant attention as potential alternate colloidal drug delivery system over conventional dosage form.
Isotretinoin, a derivative of retinoic acid (13-cis-retinoic acid), has been commonly used for the treatment of severe acne and other dermatological diseases1,2. However, it has obvious adverse side-effects by oral administration.
The launched topical preparations such as cream also show systemic absorption and significant skin irritation3. So, it is necessary to improve the skin uptake and reduce systemic absorption of isotretinoin using a carrier with an ability of skin targeting. Compared with conventional carriers such as cream, tincture and emulsion, SLN combine their advantages such as controlled release, in vivo good toleration and protection of active compounds4,5. Especially, SLN can favor drug penetration into the skins6,7, maintain a sustained release to avoid systemic absorption8, act as a UV sunscreen system9, reduce irritation10,11. Moreover, SLNs are composed of physiological and biocompatible lipids and are free from the risk of acute or chronic toxicity. Various research teams12-17 have studied the effect of lipid type on…show more content… Briefly, the lipid, soybean lecithin and isotretinoin were dissolved in ethanol. Then, ethanol as the organic solvent was completely removed using rotary evaporator (ESRA, India) at 800C. The melt residue was added to the hot water containing Tween 80. Then, the preemulsion was prepared using a high speed stirrer for 20 minutes. The pre-emulsion subsequently homogenized in a high-pressure homogenizer at 800 bar in a water bath maintained at 100C, above the melting point of the lipid. After the homogenization, the dispersion was cooled at 5-80C, the SLN dispersion stabilized by Tween 80 and soybean lecithin was
