...________________________________________________________________________ Experiment No: 01 Name of the Experiment: EXTRACTION OF OIL FROM OIL CAKE BY SOXHLET EXTRACTOR _______________________________________________________ Date of Performance: 18/11/15 Date of Submission: 25/11/15 Partners’ Std. Id- 1202012 1202013 1202014 1202015 0902058 Submitted To- Md. Nazibul Islam Lecturer, Department of Chemical Engineering, BUET Partners’ Std. Id- 1202012 1202013 1202014 1202015 0902058 Submitted To- Md. Nazibul Islam Lecturer, Department of Chemical Engineering, BUET Submitted by, Md. Touhidul Islam Std. Id- 1202011 Level- 3, Term- 1 Dept. – Ch.E Group- 03 (A1) ------------------------------------------------- 1.0 SUMMARY The objective of this experiment was to study Soxhlet Extractor and to determine the percent extraction of oil from oil cake and percent recovery of solvent used for extraction. In this experiment, a paper thimble was weighed and then measured amount of oil cake was taken in the thimble and placed in the Soxhlet holder. N-hexane, which was used as the solvent, was measured in a round bottom flask and then after setting up the apparatus, was heated by an electric heater up to its boiling...
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...Food Chemistry Laboratory report The analysis of Lipids Determination of fat by Soxhlet Extraction and Determination of fat content in milk by Gerber method Introduction Lipids are molecular organic compounds, composed largely of carbon, oxygen and hydrogen and are essential for cell growth. Lipids are non-soluble in water and combine with carbohydrates and proteins to form the majority of all plant and animal cells. Lipids are transported through the body attached to protein and are an important element of food. Lipids are the third major micronutrient required for the body after carbohydrates and protein. Lipids are also a medical term for ‘fat’ and though all fats are lipids, not all lipids are fats. Lipids have many roles within the body, one of its main duties is to transport, absorb and digest the fat soluble vitamins A,D,E and K. They provide the most energy to the body, are used for energy storage, cell membrane development, and they protect vital organs. Other major purposes are that they insulate layers under the skin, provide vitamins, essential nutrients and fatty acids in the body. Fatty acids cannot be made by the body and are therefore fat is essential in the diet. In many foods the lipid components add to flavor perception, texture, mouth feel and appearance. (Intro to food Nutrition handout, 15/02/11) It is important to remember that although fat is essential, the body only requires 30% of daily calorie intake to come from fat. Over consumption of...
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...A : DETERMINATIOPI OF CRUDE FAT OF SUNFLOWER USING SOXHLET APPAATUS ABSTRACT 11e objective of this practical was to determine the crude fat content of sunflower through semi continuous method using Soxhlet apparatus where by 5g of the sample was weighed in to extraction thimble and placed in to the extraction chamber. Then heat was applied to the flask containing the extraction solvent (ethyl ether) and the solvent builds up in the extraction chamber for 5 to 10 minutes and completely surrounds the sample then siphons back to the boiling flask. Fat content is measured by weight loss ofthe sample or by weight of fat extracted. The crude fat content was found to be 449o. Maleki et at.. (2003) reported that the crude fat content of toog of sunflower is 50°ro which is slightly higher as compared to the result obtained. This may be due to inefóciency of Soxhlet methods to extract bound lipids. INTRODUCTION The tota) lipid content of a food is commonly determined by organic solvent extraction methods. The accuracy of these methods greatly depends on the solubility of the lipid in the solvent used and the ability to separate the lipids fom complexes with other macromolecules. The lipid content of a food determined by extraction with one solvent may be quite different from the content determined with another solvent of the different polarity. In addition to solvent extraction methods there are non-solvent wet extraction methods and several...
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...KIMIA TUGAS URAIAN TANAMAN BINTARO Oleh : Jurusan Teknik Fisika Fakultas Teknologi Industri Institut Teknologi Sepuluh November Surabaya 2009 BINTARO Klasifikasi ilmiah Kingdom : Plantae (Tumbuhan) Subkingdom : Tracheobionta (Tumbuhan berpembuluh) Super Divisi : Spermatophyta (Menghasilkan biji) Divisi : Magnoliophyta (Tumbuhan berbunga) Kelas : Magnoliopsida (berkeping dua / dikotil) Sub Kelas : Asteridae Ordo : Gentianales Famili : Apocynaceae Genus : Cerbera Spesies : Cerbera manghas L. Nama binomial : Cerbera manghas BINTARO Bintaro (Cerbera manghas) adalah tumbuhan pantai atau paya berupa pohon dengan ketinggian dapat mencapai 12m. Dikenal di Pasifik dengan nama leva (Samoa), toto (Tonga), serta vasa (Fiji). Nama ilmiah Cerberus diambil dari nama anjing berkepala sepuluh dalam mitologi Yunani. Dinamakan ‘Cerbera’, karena biji Pohon Bintaro dan semua bagian pohonnya, mengandung racun yang disebut cerberin. Yakni racun yang dapat menghambat saluran ion kalsium di dalam otot jantung manusia, sehingga mengganggu detak jantung dan dapat menyebabkan kematian. Bahkan asap dari pembakaran kayunya dapat menyebabkan keracunan. Penyebarannya secara alami di daerah tropis Indo Pasifik, dari Seychelles hingga Polinesia Perancis. Bintaro sering kali merupakan bagian dari ekosistem hutan mangrove. Di Indonesia bintaro sekarang digunakan sebagai tumbuhan penghijauan daerah pantai serta peneduh kota. 1. Ciri-ciri * Habitus : Pohon, tinggi, _+ 20...
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...Chapter III Methodology 5.1 Study Design The study will utilize the Quasi experimental research, which describes and analyzes the variables that will be manipulated to influence an effect to the independent variable. Blood will be obtained from students of Medical Technology in Far Eastern University. The blood samples obtained from the student will be observed microscopically after adding the plant extract. Prothrombin time test will also be used to compare the time of coagulation between citrated plasma added with the extract and the citrated plasma alone as reference control. 5.2 Study Setting 5.3 Study Population & Sampling Technique The blood sample will be taken from 30 male or female students of Medical Technology in Far Eastern University. 9 mL of blood will be collected from consented individuals. The subjects will be asked of their medical history regarding if they are not suffering from any blood disorders, diabetes, not a smoker, not taking any medication like heparin, non-steroidal anti inflammatory drugs, and warfarin and free from any bleeding disorders. Blood Collection First, position the patient’s arm then apply the tourniquet above the antecubital fossa for 3-4 inches. Then, locate the vein and cleanse the venipuncture site with 70% isopropyl alcohol, air-dry. After drying, the tourniquet will be reapplied and the multisample needle will be inserted with the bevel up at an angle less than 30 degrees. Following the correct order of draw, citrated...
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...obtained for each sample, transferred to 500ml flask and 0.5% NaCl was added , the mixture was closed and swirled. Time was noted and the infusion was left to stand for 2,5 hours at 20°C agitating it by rotation at 20 minutes intervals. Infusion was filtered thereafter through 32cm fluted filter and first 50ml was returned to filtrate and the final 20ml was transferred to a 100ml volumetric flask and was diluted to the mark with 0,5% NaCl .10ml of solution was pippeted to a 250ml flask and brought to 20°C. 200ml of starch solution was added to the flask and maintained at 20°C for 30 minutes. 250ml of 0.5NNaOH was added and mixed by inverting the flask, and topped to the mark with distilled water. A blank solution was prepared . 10ml of Soxhlet solution and 10ml of water were added to a 200ml volumetric flask and boiled. Titration was done into simmering solution in flask, on cooling blue colour returns. Rapoko had a volume of 230mls , sorghum 270mls. Blank of rapoko had 222ml and that of sorghum had 270ml. The diastatic power of rapoko was 21°and sorghum was 18°. INTRODUCTION The diastatic power (DP), also called the diastatic activity" or "enzymatic power", of a grain in general refers only...
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...obtained for each sample, transferred to 500ml flask and 0.5% NaCl was added , the mixture was closed and swirled. Time was noted and the infusion was left to stand for 2,5 hours at 20°C agitating it by rotation at 20 minutes intervals. Infusion was filtered thereafter through 32cm fluted filter and first 50ml was returned to filtrate and the final 20ml was transferred to a 100ml volumetric flask and was diluted to the mark with 0,5% NaCl .10ml of solution was pippeted to a 250ml flask and brought to 20°C. 200ml of starch solution was added to the flask and maintained at 20°C for 30 minutes. 250ml of 0.5N NaOH was added and mixed by inverting the flask, and topped to the mark with distilled water. A blank solution was prepared . 10ml of Soxhlet solution and 10ml of water were added to a 200ml volumetric flask and boiled. Titration was done into simmering solution in flask, on cooling blue colour returns. Rapoko had a volume of 230mls , sorghum 270mls. Blank of rapoko had 222ml and that of sorghum had 270ml. The diastatic power of rapoko was 21°and sorghum was 18°. INTRODUCTION The diastatic power (DP), also called the diastatic activity " or "enzymatic power", of a grain in general refers only to malts,...
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...Standard quality analyses like Soxhlet extraction for oil content and gas chromatography for fatty acid composition are laborious, time-consuming and difficult to perform for large sample size. A non-destructive method for the screening of seed samples would also allow high throughput analysis in plant breeding and genotype selection where the seeds of a genotype are limited. Near-infrared reflectance spectroscopy (NIRS) is a technique which has been used to screen intact seed samples for oil content and fatty acid composition in different species including rapeseed and sunflower. Studies on a worldwide safflower germplasm collection and on Indian safflower genebank accessions have shown that the oil content and fatty acid composition of intact seeds can be estimated by NIRS...
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...Eur. J. Lipid Sci. Technol. 104 (2002) 785–791 William E. Neff, Fred Eller, Kathleen Warner 785 Composition of oils extracted from potato chips by supercritical fluid extraction* To determine effects of two extraction procedures on oil compositions, tocopherols, monoacylglycerol, diacylglycerol, triacylglycerol, free fatty acids, polymers and polar components were determined in oils after extraction from potato chips by either supercritical carbon dioxide or hexane. Potato chips were fried in cottonseed oil or low linolenic acid soybean oil and sampled after 1, 10 and 20 h of oil use. Both extraction methods recovered comparable amounts of oil from the potato chips. Compositions of triacylglycerol and non-triacylglycerol components including tocopherols, monomer, polymer, monoacylglycerol, diacylglycerol were similar for samples of chips fried in either oil except for the δ-tocopherol data for potato chips fried in the low linolenic acid soybean oil used for 10 h of frying. There were some differences between the composition of low linolenic acid soybean oil extracted from the potato chips compared to the fryer oil at the 20 h sampling time. These results showed that the supercritical carbon dioxide extraction gave similar results to hexane extraction in yield and composition of oils from potato chips. Keywords: Supercritical fluid extraction, oil composition, tocopherols, cottonseed oil, low linolenic soybean oil, frying, fried food. National Center for Agricultural...
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...Published in: Food chemistry (2005), vol. 91, pp. 469-476 Statut: Postprint (Author's version) Heating effects on some quality characteristics of date seed oil Besbes Souhail1, Blecker Christophe3, Deroanne Claude3, Lognay Georges4, Nour-Eddine Drira2, Hamadi Attia1 1 Unité Analyses Alimentaires, Département de biologie, Ecole Nationale d'Ingénieurs de Sfax, Route de Soukra B.P. W., 3038 Sfax, Tunisie. 2 Laboratoire de biotechnologie végétale, Faculté des Sciences de Sfax, Route de Soukra, 3038 Sfax, Tunisie. 3 Unité de Technologie des Industries Agro-alimentaires, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgique. 4 Unité de Chimie Générale et Organique, Faculté Universitaire des Sciences Agronomiques de Gembloux, passage des Déportés 2, 5030 Gembloux, Belgique. Abstract: Effects of heating on some quality characteristics of date seed oil from two cultivars (Deglet Nour and Allig) has been investigated using an accelerated test in a Rancimat system. Fatty acid, viscosity, absorptivity at 232 and 270 nm, colour and total melting enthalpy of Deglet Nour seed oil were less changed compared to that of Allig seed oil, because the former contained a higher amount of total phenolic compounds (526 µg/g against 215 µg/g) and a lower content of unsaturated fatty acids. Viscosity and absorptivity at 232 nm and at 270 nm increased rapidly after reaching the oxidation induction time. Differential scanning calorimetry...
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...Quezon City Science High School (Regional Science High School for NCR) Golden Acres Rd., Misamis st. Bago-Bantay, Quezon City SY 2013-2014 RESEARCH PROJECT Efficacy of Squash (Cucurbita maxima D) Seed Oil Extract in Lowering Blood Glucose Levels Bancolita, Joseph Albert A. De Leon, Krizia Nicolle E. Ilicito, Mari Norence G. Largoza, Marie Ysabel Beatriz M. Rahayel, Benjamin Nathanael D. Rivera, Rosa Maria L. Sidro, Keith L. ______________________ ______________________ Ms. Ma. Pilar P. Carmona Mr. Jaymar L. Galag Research Adviser Research Adviser APPROVAL SHEET In Partial Fulfillment of the requirements in Research II-B this research paper entitled, “Efficacy of Squash (Cucurbita maxima D) Seed Extract in Lowering Blood Glucose Levels” has been prepared and submitted by Joseph Albert A. Bancolita, Krizia Nicole E. De Leon, Mari Norence G. Ilicito, Marie Ysabel Beatriz M. Largoza, Benjamin D. Rahayel, and Keith L. Sidro. ______________________ ______________________ Ms. Ma. Pilar P. Carmona Mr. Jaymar L. Galag Research Adviser Research Adviser Approved and accepted in partial fulfillment of the requirements in Research II B ______________________ ______________________ Ms. Ma. Pilar P. Carmona Mr. Jaymar L. Galag Research Adviser Research Adviser ACKNOWLEDGEMENT This research project would not be feasible without the help of the following people. The researchers...
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...Acta Poloniae Pharmaceutica ñ Drug Research, Vol. 64 No. 6 pp. 547ñ552, 2007 ISSN 0001-6837 Polish Pharmaceutical Society HYPOTENSIVE EFFECT OF AQUEOUS EXTRACT OF THE LEAVES OF PHYLLANTHUS AMARUS SCHUM AND THONN (EUPHORBIACEAE). *FABIAN C. AMAECHINA and ERIC K. OMOGBAI Department of Pharmacology and Toxicology, University of Benin, Benin City, Nigeria Abstract: The plant of Phyllanthus amarus is used as diuretic and to lower blood pressure in traditional medicine practice. The effect of the aqueous extract of the leaves of Phyllanthus amarus on blood pressure was evaluated in normotensive male rabbits. Intravenously administered aqueous doses (5 mg to 80 mg/kg) of the extract to anaesthesized normotensive male rabbits produced a significant fall in mean diastolic, systolic and mean arterial pressures in a graded dose response manner. The dose of 5 mg/kg produced the least hypotensive effect, causing a fall in mean diastolic, systolic, and mean arterial pressure of 13.3 ± 3.1, 19.7 ± 5.4, and14.3 ± 3.4 mmHg, respectively, while the dose of 80 mg/kg produced the greatest fall in mean diastolic, systolic, and mean arterial pressure of 49.7 ± 7.9, 45.5 ± 9.5, and 48.00 ± 6.5 mmHg, respectively. The extract had a greater blood pressure depressant effect on the diastolic blood pressure than on the systolic blood pressure. The highest dose of 80 mg/kg caused 62.5% fall in diastolic blood pressure, compared to the 33.2% fall in systolic blood pressure caused by the same dose....
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...224 G. M. WARNOCK AND J. DUCKWVORH 1944 are not used as a source of minerals to sustain the mammary supply during lactation. In other words, an osteoporotic skeleton is not an obligatory consequence of lactation in this species at least. Considerable difficulties exist in the determination of these requirements by the metabolic balance technique, since this method does not indicate when mobilization of minerals from the spongiosa is accompanied by mobilization from the shaft. Further, the balance over a whole lactation is often small in relation to the total quantities of materials analyzed and may be close to the analytical errors of the methods when the diet given is rich in minerals. The present observations may find some application in the determination of certain mineral requirements of lactation. Because the losses of Ca and P are unavoidable, involving the gradual liberation of reserves laid down at an earlier peripd, it has been difficult to estimate the requirements for lactation on the bafsis ofthe negative values obtained by the balance technique. Since the present study shows that, in the rat, it is possible to restrict the withdrawal of bone salt to the spongiosa, leaving the shaft unaffected, it seems reasonable to suggest that lactation requirements could be defined as those intakes which, for a given output of minerals in milk, will restrict the withdrawals to the spongiosa. Thus, using a similar technique, the problem ofthe mineral requirements for lactation...
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...Asian J. Pharm. Tech. 2013; Vol. 3: Issue 1, Pg 26-29 [AJPTech.] ISSN- 2231–5705 (Print) www.asianpharmaonline.org ISSN- 2231–5713 (Online) 0974-3618 RESEARCH ARTICLE Effects of Methanolic Extracts of Quisqualis indica (Aerial Parts) on Passive Smoking Induced Hyperlipidemia in Rats Jyoti Sahu*1,2, Pushpendra Kumar Patel1,2 and Balkrishna Dubey1 T.I.T. College of Pharmacy, Department of Pharmacology, Anand Nagar, Bhopal, Madhya Pradesh, India- 462021 SBRL Indrapuri, Bhopal (M.P.) *Corresponding Author E-mail: jyotisahupharma@gmail.com 2 1 ABSTRACT: In the present study the hypolipidemic activity of methanolic extracts of aerial parts of Quisqualis indica (QI) including flowers on passive smoking (PS) induced hyperlipidemia in rats had been evaluated. Hyperlipidemia was induced by passive smoking in a closed chamber having 1 burning ciggrette inside it. The hypolipidemic activity was analysed by reading the blood serum level in UV at 505 nm after treated with reagent present in auto span diagnostic kit. Dose of Methanolic extracts of QI had been prepared by using distilled water i.e. 200 mg/kg p.o. Methanolic extracts of QI significantly reduce the harmful lipid layer in blood serum at varying concentration and dose dependent manner which shows that the plant carries the hypolipidemic properties. It reduces the LDL, VLDL, cholesterol, triglyceride and raise HDL level in blood serum upto certain extent which was may be due to the inhibition of lipid peroxidation as the plants...
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...El presente trabajo tiene la finalidad de dar a conocer los diferentes materiales de laboratorio ya que poseen distintas características, así mismo después de una ardua investigación se concluye que es muy importante el uso adecuado de los materiales de laboratorio. OBJETIVOS Familiarizarse con los materiales y equipos de laboratorio, Identificar, clasificar y establecer la utilidad de cada uno de ellos, al igual que los símbolos de riesgo y de peligrosidad, como: Identificar correctamente el material de goma, plástico, metal ó porcelana comúnmente usado en el laboratorio de petróleo. Explicar en forma clara y segura el uso del material usado en el laboratorio. Aprender a manipular adecuadamente el material de vidrio, goma, plástico, madera, metal ó porcelana. Cumplir cabalmente con las Normas de Higiene y de Seguridad del Laboratorio. Aprender a realizar lectura de volúmenes. Aprender a manipular la pipeta. Aprender a realizar la técnica del trasvasado. Formar equipos de trabajo. MATERIALES DE USO COMÚN EN EL LABORATORIO UTENSILIOS DE SOSTÉN. (UDS) Adaptador para pinza para refrigerante o pinza Holder Este utensilio presenta dos nueces. Una nuez se adapta perfectamente al soporte universal y la otra se adapta a una pinza para refrigerante de ahí se deriva su nombre. Están hechos de una aleación de níquel no ferroso Anillo de hierro Es un anillo circular de Fierro que se adapta al...
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