The specificity of albumin binding experiment was to determine the binding interactions that occur between serum albumin and three synthetic dyes with the use of electrophoretic procedure. Whole blood, or plasma. Clots upon standing and if the clot is removed, the remaining straw colored fluid is called serum. The major protein in serum is albumin which functions as a carrier molecule for the transport of certain small molecular weight compounds in blood. Molecules that bind to serum albumin are fatty acids, hormones and some synthetic dyes. In this experiment the synthetic dyes used are Bromophenol Blue, Ponceau S and Orange G. we observed that free dyes not bound to albumin migrate faster that albumin or dyes bound to albumin. This separation enabled use to differentiate between albumin-bound dye and free dye not associated with the protein. Our results were compared to Anderson to see where we failed.
Results
Group 4
This group shows the ligand binding is protein specific. We see that serum albumin binds bromophenol blue while hemoglobin doesn’t. our results corresponds very close to Anderson’s as the mixture with hemoglobin shows no binding occurred, the hemoglobin and the dye are very separated. The mixture with albumin showed binding with the dye. While the others were left as free dye. Tube/lane # | Buffer | Protein | Bromophenol blue | 1 | 20ul | 0 | 5ul | 2 | 10ul | 10ul hemoglobin | 5ul | 3 | 20ul | 0 | 5ul | 4 | 10ul | 10ul BSA | 5ul |

Fig4 . the 1st picture shows anderson results and the 2nd show the groups experiment results.
Fig4 . the 1st picture shows anderson results and the 2nd show the groups experiment results.

Group 1 and group 6
Albumin binding is saturable and ligand specific . The mixture was done in the following order and measurements the tube number corresponds with the lane the mixture is put it.
Group 1