“THE EFFECTIVENESS OF MEMORY PLUS GOLD POWDERED CAPSULE TO ENHANCE THE MEMORY OF WHITE MICE (Mus musculus)”
In partial fulfillment of the requirements in Research I
Biological Science
Team Category
Student Researchers:
Laja, Amrina R. and Laureano, Lexyleen B.
Grade 9- Special Science Class A

Research Adviser:
Ms. Rizza Earl Veverly T. Angeles
March 2015

TABLE OF CONTENTS

Abstract……………………………………………………………………………...........1
Acknowledgement…………………………………………………………………………2
Chapter I. Introduction A. Background of the Study………………………………………………......3 B. Statement of the Problem…………………………………………………..5 D. Significant of the Study…………………………………………...............6 F. Scope and Delimitation…………………………………………................7 G. Definition of Terms………………………………………………………......8
Chapter II. Review of Related Literature Memory Plus Gold………………………………………………………………..9 Why Memo Plus Gold? .………………………………………………………..10 What evidence support memo plus gold? ............................................11 Who can benefit from memo plus gold? .………………………………….. 12 Bacoppa Monniera……………………………………………………………….13 What’s in bacoppa and how does it work? …………………………………14 Mice (Mus musculus)……………………………………………………………16 Mice Protocols.………………………………………………………………....17 General Principles……………………………………………………………....18 The type of maze and what it is used for? ………………………………….20

Chapter III. Methodology A, Research Design…………………………………………………………….21 C. Procedure……………………………………………………………………22
Maze Test………………………………………………………………………..23 D. Flow Chart…………………………………………………………………...24
Chapter IV. Results and Discussion Table 1…………………………………………………………………………..25 Table 2…………………………………………………………………………..26
Chapter V. Summary, Conclusion, Findings, and Recommendation A. Summary…………………………………………………………………….27 B. Conclusion…………………………………………………………………..28 C. Recommendation…………………………………………………………...29
Bibliography……………………………………………………………………………30

Appendices A. Photo Documentation………………………………………………………32 B. Experimental Layout……………………………………………………….34 C. Raw Data…………………………………………………………………….35

Abstract
The study aims to determine whether memory plus gold powdered capsule can be an effective memory enhancer for mice to avoid memory lapses. This study sought to answer if there is a significant effect on the mice's memory before and after the intake of the memory plus gold powdered capsule.

The study used experimental method which includes all the preparation of the materials needed, gathering of the materials and testing. There were two (2) different treatments in the experiment and each treatment has three (3) replicates and three (3) samples. To compute for the statistical significance of the study for the data gathered, the researchers made use of T-Test for Two Independent Sample at 5% level of significance.

Based on the data gathered, memory plus gold powdered capsule enhances the memory of the mice. On the other hand, mice that had taken memory plus gold are faster than the mice that did not take memory plus gold in finding the food inside the maze.
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Thus, the researchers recommend using improved methodology also different dosages of the memory plus gold. The researches further recommend conducting study using different animals aside from mice.
ACKNOWLEDGEMENT
With immense pride and honor, the researchers would like to thank all the people who are in one way or another, who helped pave for the success of this research study. This study would not be possible and accomplished without the encouragement, inspiration, and support given to the researchers by the following:
First, to the Mr. Espiritu for the sponsoring of the mice which is used in this study. To the parents of the researchers, Mr. and Mrs. Laja - Mr. and Mrs. Laureano, a sense of gratitude is extended to them for their unconditional support that they have shown to the researchers as the researchers take a step in this research study. To the commentators who did not stop on giving feedbacks and suggestions for the improvement of the study, thank you.
Second, to the Department Of Science And Technology for lending the researchers some of the research write-ups in order to sort out some ideas and to mold the knowledge of the researchers for them to overwhelm the study.
Third, to Ms. Rizza Earl Veverly T. Angeles, Research Class Adviser, for the knowledgeable incites in coming up a good research study.
And above all to Our Almighty God for giving us the guidance in times when seeking help and facing challenges in this research study.
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This is such a great privilege to do this study entitled “The Effectiveness of Memory plus Gold Powdered Capsule to Enhance the Memory of the White Mice (Mus musculus)”. To all the people, once again MUCHISIMAS GRACIAS!
Chapter I
Introduction

A. Background of the Study "Forgetfulness is accepted as a natural part of getting older, but it might not have to be. Scientists say they have identified an important biological mechanism in the brain that underlies the sorts of memory lapses that come with age"(Goldman,2009).

Over the past century, the mice have developed into the premier mammalian model system for research. Scientists from a wide range of biomedical fields have gravitated to the mice because of its close genetic and physiological similarities to humans, as well as the ease with which its memory can be manipulated and analyzed.

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As a result, scientist conducted research on how to improve the memory of the mice using transitional medicines, drugs and many more. In Columbia, Stephen Small studied about the effects of virus that contained genetic material that boosted production of the protein to improve the memory of mice. They were able to find out that mice have memory lapses called RbA48 deficiencies when they get old. Some studies that is related on improving the memory of the mice are currently conducted in America and Great Britain. There are also some scientist from the Philippines who conducted the same study. Aside from using drugs, the researchers of this study would like to know if Memory Plus Gold powdered capsule can also be an effective memory enhancer for the mice thus memory plus contains Gingco binoba and Bacopa monniera that helps on improving the memory of ones living organisms.

Moreover, this can help the veterinarians here in the Philippines having a hard time with their mice due to misbehavior because of memory lapses. Hence mice do not get Alzheimer’s, but they do experience memory decline as they age. This study will be the answer to the memory problem of the mice.

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B. Statement of the Problem

This study aims to determine whether Memory Plus Gold Powdered Capsule can be an effective memory enhancer for white mice (Mus musculus). The researchers also want to clarify if there is a significant difference on white mice’s memory before and after the intake of the Memory Plus Gold Powdered Capsule in terms of the speed of the white mice in finding the food.

C. Objectives of the Study
This study aims to achieve the objectives:
1. To determine whether the memory plus gold powdered capsule can be an effective memory enhancer for white mice.
2. To determine the significant difference of the memory of white mice before and after the intake of Memory Plus Gold.

5 D. Significant of the Study
The primary beneficiaries of this study are the citizens of the Philippines especially veterinarians and pet shop entrepreneur. This study can give the readers the knowledge that not only humans can improve their memory but also mice can.
The use of memory plus gold powdered capsule can prevent the risk of the memory lapses of a certain white mice. E. Hypothesis of the Study

There is no significant effect on the memory of white mice before and after the intake of Memory Plus Gold Powdered Capsule.

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F. Scope and Delimitations
The memory plus gold capsule were bought from the United Pharmacy at Gov. Ramos Street, Sta. Maria Zamboanga City. The white mice were bought from Renz’s Pet shop fronting the Safety Enterprises, Canelar, Zamboanga City The research was conducted at Laureano's Residence located at Saavedra Compound, Lower Cabatangan, Zamboanga City.
The experiment has two (2) different treatments and three (3) replicates to ensure the accuracy of the results. The experiment lasted for five (5) days from March 2, 2015 to March 6, 2015 which includes the preparation and gathering of the materials and testing.
To compute for the significant effect of the memory plus gold powdered capsule for the improvement of the memory of white mice T-test for two Independent sample at 5% level of significance was used to measure the statistical significance of the data. Thus, this study is only tested and proven for mice. There were no other species used aside from white mice.

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G. Definition of Terms 1. Memory Plus Gold refers to a natural memory enhancer and brain power builder. It is formulated from the extract of Bacopa monniera (commonly known as Brahmi) developed and patented by the Central Drug Research Institute (CDRI) in India. It facilitates learning, improves consolidation of learned behavior and delays its extinction by enhancing protein kinase activity and new protein synthesis, particularly in brain cells related to memory. 2. Memory Lapses refers to minor failure of memory slip. 3. White Mice refers to any of numerous small rodent of various families, having small ears and a long thin tail ,esp. an Old World mouse, Mus musculus, introduced worldwide. 4. Alzheimer's Disease refers to a disease of brain that causes people to slowly lose their memory and mental abilities as they grow old.

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Chapter V
SUMMARY, CONCLUSION, FINDINGS, AND RECOMMENDATION

A. Summary This study aimed to determine whether Memory plus Gold Powdered Capsule can be used as a memory enhancer for white mice which includes two different treatments (First treatment is not introduced with Memory Plus Gold while the second treatment is introduced with it). The study employed the experimental method wherein it includes gathering materials, acquisition of mice, labeling of mice, maze testing for both treatments before the introduction of Memory Plus Gold, Introduction of Memory Plus Gold to treatment no.2, maze testis for both treatments after the introduction of Memory Plus Gold, and observation. The study made used of T-test for Two Independent Sample at 5% level of significance to compute for the statistical significance of the results. The results showed that Memory Plus Gold can be an effective memory enhancer for white mice. In addition, white mice that were introduced with memory plus gold were faster in finding the food inside the maze.

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Findings
The following were the major findings of the study: 1. Memory plus gold is an effective memory enhancer for white mice thus it enhances well the memory of white mice. 2. There is a significant difference on white mice’s memory before and after the intake of the Memory Plus Gold Powdered Capsule in terms of the speed of the white mice in finding the food.

B. Conclusion
Based on the findings of the study, the researchers therefore conclude that Memory Plus Gold created from Bacopa monniera enhances the memory of the mice in terms of their ability to solve the maze, to navigate, and to search for food. The plant Bacopa monniera highly contributed in the effectiveness of the drug, from which this is supported by the big difference in the speed of white mice introduced with Memory Plus Gold and with no Memory Plus Gold implied that it has the potential to be a memory enhancer, specifically for the mice. The speed was not also consistent, because based from the gathered data, the more times the mice were exposed in the maze the faster they were. Also with each day mice introduced with no Memory Plus Gold tend to go to the exit of the maze faster, rather than the mice with no Memory Plus Gold introduced. From which it can also be said the speed of the mice also dependent on the amount of Memory Plus Gold given to the mice. 28 C. Recommendations For further studies related to this research, the researchers recommended that a comparison of different amounts of Memory Plus Gold be tested. Also, using other animals aside from white mice. The researchers also recommend to use more complicated maze and other products or methods that could possible enhance the memory of animals or white mice. Since the researchers used basic equipment throughout the study, it is recommended that for future studies use more advanced materials and laboratory apparatuses.

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Appendices A. Photo Documentation
In handling of mice, surgical gloves should be worn to avoid accidents.
In handling of mice, surgical gloves should be worn to avoid accidents.
Treatment No. 2: Mice treated with Memory Plus Gold.
Treatment No. 2: Mice treated with Memory Plus Gold.
Treatment No. 1: Mice with no intake of Memory Plus Gold.
Treatment No. 1: Mice with no intake of Memory Plus Gold.

The maze used by the researchers for the testing of the memory of the tested mice.
The maze used by the researchers for the testing of the memory of the tested mice.

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First day of intake of Memory Plus Gold Powdered Capsule. March 3, 2015
First day of intake of Memory Plus Gold Powdered Capsule. March 3, 2015
First testing of Treatment No. 1. This was taken on March 2, 2015 at exactly 9:36 AM
First testing of Treatment No. 1. This was taken on March 2, 2015 at exactly 9:36 AM
Last day of testing the speed of the mice for the both treatments. March 6, 2015 at exactly 5:27 AM
Last day of testing the speed of the mice for the both treatments. March 6, 2015 at exactly 5:27 AM
Testing after the intake of Memory Plus Gold. March 5, 2015 at exactly 3:32 PM (Treatment No.2)
Testing after the intake of Memory Plus Gold. March 5, 2015 at exactly 3:32 PM (Treatment No.2)

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B. Experimental Layout

1. Research Title: “THE EFFECTIVENESS OF MEMORY PLUS GOLD POWDERED CAPSULE TO ENHANCE THE MEMORY OF WHITE MICE (Mus musculus)” 2. Identify the Independent Variable: the application of Memory Plus Gold 3. Identify the Dependent Variable: the speed of the white mice after the introduction of Memory Plus Gold 4. Determine the number of the following: a. Number of Treatments: 2 b. Number of Replicates: 3 c. Number of samples per replicates: 3 d. Total number of samples: 2x3x3=18 5. The speed of white mice (Dependent vsariable)
The speed of white mice (Dependent vsariable)
The application of memory plus
(Independent Variable)
The application of memory plus
(Independent Variable)
Construct a conceptual framework showing the cause and effect of the IV and DV:
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Speed of mice after the intake of Memory Plus Gold
Speed of mice after the intake of Memory Plus Gold
Speed of mice before the intake of Memory Plus Gold
Speed of mice before the intake of Memory Plus Gold
100% Memory Plus Gold
100% Memory Plus Gold
Experimental (Memory plus gold)

Experimental (Memory plus gold)

Control (Untreated)

Control (Untreated)