Premium Essay

Trp Operon

In:

Submitted By williannyg
Words 404
Pages 2
In this discussion, you will describe an operon. There are many examples listed in Chapter 14, but if you want to choose an operon not described in your book, please ask me for approval. Make sure you do not pick the same operon as your classmates; we want to expose ourselves to as many regulation proteins and bacteria as possible.
In your initial discussion board post, please include the following: The Tryptophan(trp) Operon
1. Describe an operon, the regulatory proteins, the cellular process and the molecules being “sensed”.
2. Include details about the orientation of the genes and the promoter/operator sequences or activator binding site.
3. Provide details about the repressor/activator and inducer/inhibitor proteins.
4. Explain how the operon helps microbe survive and possibly thrive in its environment.
5. Include details about conditions that trigger expression of the gene(s).
6. Explain when this regulation occurs: During transcription, translation, or posttranslation
An operon is the genetic regulatory system found in bacteria and their viruses where genes coding for functionally related protein are clustered along the DNA. This allows protein synthesis to be controlled coordinatly in response to the needs of the cell. The trp operon in E. coli us a negative control repressible system, the trp operon is negative control because the system produces a repressor which functions to turn off the operon. The trp operon is a repressor operon because repression occurs when a critical substance is abundant in a cell. Trp is an important amino acid which most E. coli acquire by extracting from the material the cell consume or by manufacturing themselves. The genes controlled by the trp produce enzymes which are necessary for the cell to produce its own tryptophan.
The componets of the trp operon are the same as the ones in the lac operon. the

Similar Documents

Free Essay

Operon

...Operons Control of Gene Activity in Prokaryotic Cells I. The activity of genes is controlled by the cell and the environment. A. Inducible genes are inactive unless circumstances cause them to be activated (“turned on”). B. Repressible genes are active unless circumstances cause them to be inactivated (“turned off”). C. Constitutive gene functions are active continually, with no control exerted. This is generally an abnormal situation. II. In prokaryotic cells (and viruses) the control of gene activity is often in the form of operons. A. Operons are a form of transcriptional control. B. An operon consists of the structural gene (or genes) which actually code for specific proteins and the controlling elements associated with the control of those genes. An operon typically contains several genes, all under the same control mechanism. C. Though rather similar controlling systems have been found for some eukaryotic genes, control mechanisms in eukaryotes are generally more diverse and more complex, and except for a few examples in simple eukaryotic organisms like yeasts, multiple genes are not found to function under a single control mechanism. In other words, eukaryotic cells do not have operons. III. The first operon investigated was the lac operon in E. coli. This work came from Jacob and Monod (1959 Journal of Molecular Biology V. 1). A. The primary carbohydrate source for the cell is the sugar glucose, but there...

Words: 2059 - Pages: 9

Premium Essay

Molecular Genetics Recombination

...by configuring clusters of genes together on the genome into operons that elicit a processive response in the presence of a specific metabolite. The Lac operon is responsible for the cleaving of the disaccharide lactose into two products. A myriad of components control the expression of the Lac operon when two conditions are met. First, the substrate, lactose, must be present. Second, no better substrate for example, glucose, is present (2). The three structural genes in the Lac operon are lacZ, lacY, and lacA. The gene lacZ encodes the tetramer, ß-galactosidase, which is responsible for hydrolyzing the ß-1,4 glycosidic linkage between galactose and glucose in lactose. The transport of lactose into the cell via the enzyme lactose permease is encoded by the gene lacY. The lacA gene encodes the enzyme, galactoside transacetylase, a trimer that transfers an acetyl group from acetyl-CoA to galactosides. Activation of these genes is dependent on the activity of a promoter and three operators based on the nutritional and environmental conditions available to the cell. The lac operon is a negatively controlled inducible operon that utilizes the product of the regulator gene lacI, to repress RNA polymerase from transcribing the lacZYA genes. The three operators involved in the lac operon: O1, O2, and O3 serve as binding sites for lacI and when bound repression is exhibited. Originally, the Jacob-Monod model of the Lac operon proposed only one operator existed. However, with the discovery...

Words: 10690 - Pages: 43

Free Essay

Gene Regulation

...PYF12 3/21/05 8:04 PM Page 191 Chapter 12 Gene expression and regulation Bacterial genomes usually contain several thousand different genes. Some of the gene products are required by the cell under all growth conditions and are called housekeeping genes. These include the genes that encode such proteins as DNA polymerase, RNA polymerase, and DNA gyrase. Many other gene products are required under specific growth conditions. These include enzymes that synthesize amino acids, break down specific sugars, or respond to a specific environmental condition such as DNA damage. Housekeeping genes must be expressed at some level all of the time. Frequently, as the cell grows faster, more of the housekeeping gene products are needed. Even under very slow growth, some of each housekeeping gene product is made. The gene products required for specific growth conditions are not needed all of the time. These genes are frequently expressed at extremely low levels, or not expressed at all when they are not needed and yet made when they are needed. This chapter will examine gene regulation or how bacteria regulate the expression of their genes so that the genes that are being expressed meet the needs of the cell for a specific growth condition. Gene regulation can occur at three possible places in the production of an active gene product. First, the transcription of the gene can be regulated. This is known as transcriptional regulation. When the gene is transcribed and how much it is...

Words: 9991 - Pages: 40

Premium Essay

Genetics

...Problems from textbook 13.18 The streptomycin sensitive strain should be exposed to a mutagen (such as UV light or radiation) to create mutants that may be resistant to streptomycin. Next, prepare a plate that has streptomycin. The bacterial colonies that grow on this plate will all be resistant to streptomycin. Now, make a copy of these colonies using replica plating. Transfer the colonies to a plate that does not contain streptomycin. The colonies that grow on the plate without streptomycin are the strain that can live with or without streptomycin. The colonies that do not grow on the plate without streptomycin cannot live without streptomycin. 13.40 The original polypeptide strain and the double mutant differ in two amino acids. The Lys-Gly amino acids in the original polypeptide become Glu-Arg in the double mutant. The Lys-Gly amino acid sequence is: AAA-GGG By adding a G before the first A in the initial AAA sequence, and by deleting the final G, we get: GAA-AGG The resulting sequence codes for Lys-Arg. The entire nucleotide sequence in the double mutant is: 5’ – AUG CCC UUU GGG GAA AGG UUU CCC UAA—3’ 14.8 There are two genes. Gene 1: mutants 1,2,3,4,5,6,8 Gene 2: mutants 7 14.14 A cis-trans test can be performed to determine whether the two varieties are the results of mutations on the same gene, or on different genes. First, it is necessary to ensure that each white variety is true-breeding. Next, we need...

Words: 1393 - Pages: 6

Premium Essay

Unit 6: Gene Expression

...bacteria is the operon. Explain the function of an operon and why it is an evolutionarily advantageous for bacteria. 2 points. Operon is a unit made up of linked genes that is thought to regulate other genes responsible for protein synthesis. A Operon allows protein synthesis to be controlled in response to when the cell needs it. Energy is conserved due to the fact that it is in control of when protein is needed. A Operon is made up of a group of structural genes that codes for enzymes that are...

Words: 1635 - Pages: 7

Free Essay

Managment

...Choice Questions 1. Regarding the lac operon, if lactose is present, which of the following occurs? A) Lactose binds to the operator preventing the promoter from attracting RNA polymerase and preventing transcription. B) Lactose bind to RNA polymerase, which then binds to the promoter and transcribes the needed genes. C) Lactose binds to the repressor, which does not bind to the operator, and RNA polymerase transcribes the needed genes. D) Lactose binds to the operon, which attracts RNA polymerase, then transcription of the needed genes occurs. E) Lactose binds to the CAP site to prevent the CAP protein from binding Answer: C 2. Which of the following is likely to be expressed? A) euchromatin B) heterochromatin C) DNA without methyl groups D) DNA with many methyl groups E) euchromatin and DNA without methyl groups is more likely to be expressed Answer: E 3. Which of the following is a method of posttranscriptional control? A) transcription factors B) the life span of a mRNA molecule C) differential processing of mRNA D) how fast the mRNA leaves the nucleus E) both differential processing and how fast mRNA leaves the nucleus are involved in posttranscriptional control. Answer: E 4. Which gene in an operon is incorrectly matched with its function? ...

Words: 2003 - Pages: 9

Free Essay

Biology: Concepts and Connections 5e Chapter 11

...extracellular matrix. E) the position of cells within an embryo. Topic: Introduction Skill: Conceptual Understanding 3) The term gene expression refers to the A) fact that each individual of a species has a unique set of genes. B) fact that individuals of the same species have different phenotypes. C) process by which genetic information flows from genes to proteins. D) fact that certain genes are visible as dark stripes on a chromosome. E) flow of information from parent to offspring. Topic: 11.1 Skill: Conceptual Understanding 4) In a prokaryote, a group of genes with related functions, along with their associated control sequences, defines A) an allele. B) an operon. C) a locus. D) a transposon. E) a chromosome. Topic: 11.1 Skill: Factual Recall 5) The lac operon in E. coli A) prevents lactose-utilizing enzymes from being expressed when lactose is absent from the environment. B) coordinates the production of tryptophan-utilizing enzymes when it is present. C) allows...

Words: 3118 - Pages: 13

Free Essay

Ttttt

... the free encyclopedia Properties Chemical formula C11H12N2O2 Molar mass 204.23 g·mol−1 Solubility in water Soluble: 0.23 g/L at 0 °C, 11.4 g/L at 25 °C, 17.1 g/L at 50 °C, 27.95 g/L at 75 °C Solubility Soluble in hot alcohol, alkali hydroxides; insoluble in chloroform. Acidity (pKa) 2.38 (carboxyl), 9.39 (amino)[1] Supplementary data page Structure and properties Refractive index (n), Dielectric constant (εr), etc. Thermodynamic Phase behaviour data solid–liquid–gas Spectral data UV, IR, NMR, MS Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).  verify (what is:  / ?) Infobox references Tryptophan (IUPAC­IUBMB abbreviation: Trp or W; IUPAC abbreviation: L­Trp or D­Trp; sold for medical use as Tryptan)[2] is one of the 22 standard amino acids and an essential amino acid in the human diet. It is encoded in the standard genetic code as the codon UGG. Only the L­stereoisomer of tryptophan is used in structural or enzyme proteins, but the R­stereoisomer is occasionally found in naturally produced...

Words: 5521 - Pages: 23

Premium Essay

Sasdfasdf

...MCDB1A Lecture Schedule, Fall 2013  Part 1: Introduction to Biochemistry and Molecular Biology Dr. Stuart Feinstein Email: stu.feinstein@lifesci.ucsb.edu Office: Bio II, Rm. 5123; Office Hours: T and F after Lecture, in front of Campbell Hall and by appointment (after Oct. 22, T at 9 AM in Bio II, Rm. 5123). Approximate Lecture Schedule for Biochemistry/Molecular Biology  Date |  TOPIC | Reading in Text*[chapter (pages)] |   |   |   | 9/27 | Introduction to class and Biochemical Principles | 1 (1-20); 2 (21-37) |   |   |   | 9/30 | Introduction to Macromolecules/ Lipids and Polysaccharides | 3 (39-42; 51-61);6 (105-109) |   |   |   | 10/1 | Macromolecules: Polysaccharides and Proteins | 3 (42-51) |   |   |   | 10/2 | Macromolecules: Proteins and Nucleic Acids | 4 (62-67);13 (259-280) |   |   |   | 10/4 | Macromolecules: Nucleic acids as Genetic Material | 13 (259-280) |   |   |   | 10/7 | The Central Dogma: DNA  Replication | 13 (259-280) |   |   |   | 10/8 | The Central Dogma: Transcription and Translation | 14 (281-303) |   |   |   | 10/9 | The Central Dogma: Translation and Mutations | 14 (281-303)15 (304-308) | 10/10 | First Honors Meeting  |   | 10/11 | The Central Dogma: Post-Translational Modifications, Alternative RNA Splicing and MicroRNAs | 14 (300-304;312-313) 16 (346-349)  |   |   |   | 10/14 | Energy, Enzymes and Metabolism | 8 (144-164) |   |   |   | 10/15 | Chemical Pathways that Harvest Chemical Energy | 9 (165-184)...

Words: 726 - Pages: 3

Premium Essay

Beautiful Mind

...Match the following. Write answer in given space. [1M ( 5] (i) Ciproflaxacin (a) inhibits protein synthesis (ii) Ethambutol (b) inhibits RNA synthesis (iii)Intein (c) Inhibits incorporation of mycolic acid in membrane (iv)Nitrosomanas (d) does not appear in functional RNA (v)Rifampicin (e) does not appear in functional protein (f) carry out nitrogen fixation (g) member of nitrifying bacteria Ans: (i) (ii) (iii) (iv) (v) Q2. Write 'T' for true and 'F' for false statement/s given below. [1M ( 5] (i) Bactoprenol is a lipid carrier used in synthesis of peptidoglycan. (ii) Disinfectant is used to remove pathogenic bacteria from inanimate objects. (iii) trp operon contains leader sequence which can be translated. (iv) After death of nodulated plant, rhizobial bacteriod released in soil colonize other plant and continue cycle. (v) Secondary treatment of sewage water is truly biological event. Ans: (i) (ii) (iii) (iv) (v) Q3. Scientists working with DNA in vitro often store it in solutions that contain...

Words: 430 - Pages: 2

Free Essay

My Paper

...Chapter 6 Blast program for sequence comparisons and blast p-values- test whether 2 or more sequences (protein or DNA) share a common evolutionary origin (p >10^-3 = due to chance) Lack of relationship between number of genes in a genome and its biological complexity 10-nm versus 30-nm chromatin fibers – condensed chromatin= 30nm wide, “beads-on-a-string” =10nm wide nucleosome core histone composition (2 each of H2A, H2B, H3, and H4) – Histones exists as octamers. Core is wrapped by 147 bp, about 2turns of DNA= CONSERVED IN ALL EUKARYOTES two turns of DNA around histone core (147 bp) variable size of DNA between nucleosomes (15-90 bp) – depends on species structure of 30 nm fiber and role of H1 histone – resting chromatin will be 30nm wide, H1 binds where DNA enters and exits nucleosome core histone tail modifications (acetylation, methylation, phosphorylation) – methylation & DEacetylation  condensing of chromatin (30nm)   acetylation  DE-condensing of chromatin (10nm)   phosphorylation & ubiquitination  chromatin remodeling euchromatin versus heterochromatin chromosome scaffold – hold the 30nm chromatin loops attached, genes far apart on the chromosome are close at the base of the loops called SARS (Scaffold Associated Proteins) width of fully condensed metaphase chromosomes (500-750 nm) – 500-750nm wide chromosome banding and FISH (fluorescence in situ hybridization) – identification of karyotypes (chromosome composition)  allows painting of each...

Words: 4186 - Pages: 17

Premium Essay

Research

...Review for BSC1010 Final that will consist of 50 questions taken from these facts. 1. A modified form of cellulose found in the exoskeletons of insects and crustaceans is known as chitin. 2. Non-polarity and insolubility characterize biological compounds known as lipids. 3. Alpha helix and beta pleated sheets are examples of the secondary level of protein structure. 4. Components of nucleic acids include a five-carbon sugar, a phosphate group, an organic nitrogen containing base, and phosphodiester bonds. 5. Fructose and galactose all have the same six carbon atoms, twelve hydrogen atoms, and six oxygen atoms, just as glucose, but they are named differently because they are alternate forms of the monosaccharide, glucose . 6. Nucleic acids are created with phosphodiester bonds. 7. Nitrogen and carbon are common to all organic molecules. 8. Fuels such as gasoline are nonpolar and high in energy because they are largely composed of hydrocarbons. 9. Lipids serve as important energy stores and is a major component of plasma membranes. 10. The functional group -COOH has acidic properties and would release hydrogen ions in an aqueous (water) solution. 11. The peptidoglycan of the bacterial cell wall contains a carbohydrate matrix linked together by short chains of amino acids. 12. Nuclear pores apparently permit the passage of only proteins inward and outward, but RNA only outward. 13. Mitochondria and chloroplasts are the other organelles besides the nucleus that contain DNA. 14. The...

Words: 3437 - Pages: 14

Premium Essay

Biochemistry Test Bank Questions

...Contents Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Chapter 11 Chapter 12 Chapter 13 Chapter 14 Chapter 15 Chapter 16 Chapter 17 Chapter 18 Chapter 19 Chapter 20 Chapter 21 Chapter 22 Chapter 23 Introduction to Biochemistry Water Amino Acids and the Primary Structures of Proteins Proteins: Three-Dimensional Structure and Function Properties of Enzymes Mechanisms of Enzymes Coenzymes and Vitamins Carbohydrates Lipids and Membranes Introduction to Metabolism Glycolysis Gluconeogenesis, The Pentose Phosphate Pathway, and Glycogen Metabolism The Citric Acid Cycle Electron Transport and Oxidative Phosphorylation Photosynthesis Lipid Metabolism Amino Acid Metabolism Nucleotide Metabolism Nucleic Acids DNA Replication, Repair, and Recombination Transcription and RNA Processing Protein Synthesis Recombinant DNA Technology 1 10 27 46 65 85 104 119 137 153 169 185 199 213 227 241 256 269 284 300 315 330 348 Chapter 1 Introduction to Biochemistry 1) Which elements account for more than 97% of the weight of most organisms? A) C, H, N, Mg, O, S B) C, H, N, O, P, S C) C, H, N D) Fe, C, H, O, P E) Ca2+ , K+ , Na+ , Mg 2+ , ClAnswer: B Page Ref: Section 2 2) Proteins in biological membranes may be A) porous. B) attached to the membrane surface. C) span the membrane. D) All of the above E) B and C only Answer: D Page Ref: Section 3 3) Which statement...

Words: 70772 - Pages: 284

Free Essay

So Rules 2013

...ANATOMY AND PHYSIOLOGY- 2013 Draft v4 7-3-12 1. DESCRIPTION: Understand the anatomy and physiology of the nervous, excretory and digestive system. A TEAM OF UP TO: 2 APPROXIMATE TIME: 50 Minutes 2. EVENT PARAMETERS: Each team may bring only one 8.5" x II" two-sided page of notes that contain information in any form from any source and up to 2 non-programmable, non-graphing calculators. 3. THE COMPETITION: Students should know the basic anatomy and physiology of the nervous, excretory and digestive systems and how aging and specific diseases affect them. Process skills expected may include data collection, making observations, inferences, predictions, calculations, analyses and conclusions. The test may include various formats (e. g., timed stations, written test, PowerPoint slides, anatomical specimens, etc.) for the following topics: a. DIGESTIVE SYSTEM - All levels should know: 1. Functions ofthe digestive system 11. Basic anatomy of the component parts of the alimentary canal and accessory organs of digestion iii. Anatomy of the four layers ofthe wall of the alimentary canal IV. Comparison of the lining of the esophagus, stomach, small intestine and large intestine v. Compare and contrast mechanical and chemical digestion vi. Physiology of chemical digestion of proteins, fats and carbohydrates vii. The effects of exercise on the digestive system viii. The diseases on each level from the cell to the whole person as listed: stomach & duodenal ulcers, cancers of the digestive...

Words: 20546 - Pages: 83