The settlement was located within the country of Tsenacommacah, which was ruled by the Powhatan Confederacy, and specifically in that of the Paspahegh tribe. The natives initially welcomed and provided crucial provisions and support for the colonists, who were not agriculturally inclined. Relations with the newcomers soured fairly early on, leading to the total annihilation of the Paspahegh in warfare within 3 years. Mortality at Jamestown itself was very high due to disease and starvation, with over 80% of the colonists perishing in 1609–1610 in what became known as the "Starving Time".[4]

The Virginia Company brought eight Polish[5][6][7][8][9][10][11][12] and German colonists in 1608, in the Second Supply, some of whom built a small glass