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Unit 3.1 Biology Lab Report

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The present study was conducted to determine the characteristics of the Antartic yeast Glaciozyma antarctica PI12. Our results indicated that G. antarctica PI12 required 48 hours to reach its mid-log phase, with a doubling time of 15.8 hours per generation. Our result also indicated that G. antarctica PI12 grew better under aerobic condition than anaerobic condition. G. antarctica PI12 was able to grow and proliferate at -5oC and -7oC for at least five months. The cells entered mid-log phase around 3-4 months at those temperatures. Mature cells at the mid-log phase were 14.86 µm in length, and have an area side of 65.42 μm2. Whereas for bud cells were 4.86 µm in length, and have an area side of 10.52 µm2. For both mature cell and bud cell, the diameter was …show more content…
Microscopic analysis via fluorescent microscope revealed that cell division occurred in either pole of the cell. This result indicated that G. antarctica PI12 is a basidiomycetes yeast. The SEM photos revealed the bud scar at the polar ends, which support the result of the fluorescent microscopic analysis. The genome Glaciozyma antarctica PI12 has been sequenced, but the transcriptomic study discovered numerous transcripts could not map back to the genome. The unmapped transcripts are probably transcribed by genes in the extrachromosomal DNA which was not sequenced previously. This piece of extrachromosomal DNA was amplified using the method Rolling Circle Amplification (RCA). The amplicons were fragmented into smaller fragments using ultrasonic device, prior insert into a cloning vector for sequencing. Eventually, it was found that the extrachromosomal DNA was possibly a circular DNA molecule, which was attached to a ~6kb

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