...Introduction The profound importance for microorganisms to operate at a maximum efficiency has lead to adaptations allowing for groups of processes to be functional when resources are available, while on the contrary remaining “dormant” when not in need. This has been accomplished at the molecular level by configuring clusters of genes together on the genome into operons that elicit a processive response in the presence of a specific metabolite. The Lac operon is responsible for the cleaving of the disaccharide lactose into two products. A myriad of components control the expression of the Lac operon when two conditions are met. First, the substrate, lactose, must be present. Second, no better substrate for example, glucose, is present (2). The three structural genes in the Lac operon are lacZ, lacY, and lacA. The gene lacZ encodes the tetramer, ß-galactosidase, which is responsible for hydrolyzing the ß-1,4 glycosidic linkage between galactose and glucose in lactose. The transport of lactose into the cell via the enzyme lactose permease is encoded by the gene lacY. The lacA gene encodes the enzyme, galactoside transacetylase, a trimer that transfers an acetyl group from acetyl-CoA to galactosides. Activation of these genes is dependent on the activity of a promoter and three operators based on the nutritional and environmental conditions available to the cell. The lac operon is a negatively controlled inducible operon that utilizes the product of the regulator gene lacI, to...
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...Introduction: The purpose of this experiment was to successfully transform pGLO plasmid into E.Coli cells. In the first segment of this laboratory exercise, one had to carry out a restriction digest. Restriction digestion is the process of cutting DNA molecules into smaller pieces with special enzymes such as, BamH1 and EcoR1. One had to determine which of the two plasmids, A or B, were pGLO or pWEB. A plasmid is a small circular DNA strand in the cytoplasm of a bacterium (Isite, 2013). In order to determine that, one had to use BamH1 and EcoR1 on two tubes each one with plasmid A and the other with plasmid B to observe the cuts made by each enzyme. The hypothesis for this segment of the laboratory exercise states that pGLO was plasmid B, and pWEB was plasmid A. PGLO is a plasmid which contains green fluorescent protein (GFP). GFP is found in the Aquarius Victoria jelly fish. These jelly fish have a bioluminescent protein that emits blue light. GFP converts the blue light into green light, and that is why these jelly fish emit green light. The pGLO was inserted into E.coli by using transformation. Transformation is the process of transferring genetic material between microbial cells (Tu 2008). Bacterial cells need to be in a state of competency prior to transformation (Isite 2013). Some bacteria naturally achieve this stage when nutrients and oxygen are low (Isite 2013). In the laboratory, bacteria were artificially induced with calcium chloride to be competent for transformation...
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...The Effects of Calorie Restriction on Aging by Jessica Swantek Introduction For ages, humans have been searching for ways to counteract the aging process. The legendary fountain of youth generated much attention in the past, and more recently, thousands of dollars have been spent each year on creams, pills, plastic surgery, and various forms of therapy designed to make one look and feel younger. So far nothing has been proven to reverse or even retard human aging, but scientists are finally catching a glimpse as to a dietary manipulation technique that might work. Preliminary Experimentation In the 1930s, Clive McCay, a scientist at the laboratories at Cornell University, experimented on his rats by feeding them less than they would ordinarily take for themselves, but without depriving them of nutrition to the point of starvation. He found that the food-deprived rats lived considerably longer than expected for a standard rat's life span, and about 33 percent longer than his control group of rats, which were fed as much as they wanted to eat (Weindruch 46). McCay didn't fully understand his results, and although published, they were generally disregarded by the science world (Man Immortal). Years later, Roy Walford, a nutritionist working at the University of California at Los Angeles Medical Center, came across the documentation of McCay's experiments, and, using modern technology and mice instead of rats, picked up where McCay had left off (Man Immortal). Walford found...
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...The Effects of Calorie Restriction on Aging by christiano Introduction For ages, humans have been searching for ways to counteract the aging process. The legendary fountain of youth generated much attention in the past, and more recently, thousands of dollars have been spent each year on creams, pills, plastic surgery, and various forms of therapy designed to make one look and feel younger. So far nothing has been proven to reverse or even retard human aging, but scientists are finally catching a glimpse as to a dietary manipulation technique that might work. Preliminary Experimentation In the 1930s, Clive McCay, a scientist at the laboratories at Cornell University, experimented on his rats by feeding them less than they would ordinarily take for themselves, but without depriving them of nutrition to the point of starvation. He found that the food-deprived rats lived considerably longer than expected for a standard rat's life span, and about 33 percent longer than his control group of rats, which were fed as much as they wanted to eat (Weindruch 46). McCay didn't fully understand his results, and although published, they were generally disregarded by the science world (Man Immortal). Years later, Roy Walford, a nutritionist working at the University of California at Los Angeles Medical Center, came across the documentation of McCay's experiments, and, using modern technology and mice instead of rats, picked up where McCay had left off (Man Immortal). Walford found that...
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...calories are minimalized to a couple hundred calories and on the other day you would eat as normal. This diet is particularly best for weight loss due to longer periods of food restriction. The next fasting approach is called the Warrior Diet, which is based on the concept of fasting for 20 hours and promotes eating one large, healthy meal at the end of the day (Simmons). “It claims that this pattern of eating is in sync with humans’ circadian rhythm and will promote general health while ‘removing harmful toxins from the body’” (Simmons 2014). The fasting period allows you to consume small snacks such as fruits and veggies and some high protein snacks or shakes. The common factor among each one of the fasting diets is calorie restriction, which eventually leads to weight loss. Because meals are limited to a certain timeframe, ideally the calorie consumption is going to be less when following a proper diet. Eliminating multiple meals throughout the day until mealtime or “feeding” time is going to reduce added calories to one's diet. The restrictive diet helps reduce the risk factors associated with being overweight and obese such as certain cancers and diabetes (Patterson 2015). Overall fasting is not going to be the most beneficial way to lose weight by itself. Other factors such as calorie restriction and increased metabolism contribute to weight loss. For individuals who are looking for significant weight loss fasting may not be the best plan. In fasting, reducing the eating...
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...Marxism and almost every point. The only different is their change in violent tactics. Fabian Socialism promatoe “a more peacefull, gradual, and incremental approach to encomic change through voting, legislation, the courts, and the public education system, as well as organized industrial labor”. (Ferdon 2014) Fabian Sociamlisms was introduced into the United states first is 1886. This is when the American Federation of labor was created. In 1905 the intercollegiate socialist society was formed. This is only a few ways Fabian Socialism was promoted in the United States. This all goes with the Fabian Socialism views on, labor, education, voting, and the courts. The current Democratic Party platform has been impacted by the process philosophy of Fabian Socialism. The Platform has views that are the same views of the Fabian Socialists. If you look at the views on economic change through voting, legislation, the courts, and the public education system, you will better understand how they are similar. The current Democratic Party platform is the based solely on the Fabian Socialism philosophy. I will explain this by starting with voting. The Fabian Socialists wanted encomic change through voting. This is also the case with the current Democratic Party. This Party has strong views on changing voting laws and making it easier for people to vote. “We believe the right...
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...Helder Andrade Prof. O’Donnell State & Local Politics 10/20/2014 Voting Requirements Effect on Turnout The right to vote is a very important aspect of United States government, the voting process present itself as the representative act that the fundamental basis to our Democratic system here in the USA. Unfortunately if you go back not even a century ago African Americans and women was denied this fundamental right to vote, securing the right to vote in United States was a long battle both for African-Americans and women. We can see how voting outcomes is essential to the true democratic process by having tight voting requirement it hinders the process and effects turnout. Voting Turnout, while undergoing new legislation since the repeal of the Jim Crow Laws, are still undergoing voting suppression, still disproportionately effecting racial minorities and the poor. To truly understand how voting requirements effect voter’s turnout we would have to look at the history of voting requirements in the U.S. Throughout United States history there have been many barriers that states have imposed that restrict voting rights especially for racial minorities and the poor. One requirement that definitely made voting a little more difficult was with requiring Americans to register prior to voting this meant that not only a citizen was care enough to go out and vote on election night they also must register prior to doing so. Registration was mainly intended to stop voter fraud...
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...identification is a logical step in a country that requires photo ID for everything from cashing checks to buying cold medicine: Shouldn’t there be a basic protection for the integrity of the voting process”(qtd. in Dean.) Most states at the moment require only a spoken name to be eligible to vote. This results in abuses which harm the election process. The US government should implement voter ID laws in order to protect voter integrity and ensure accurate...
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...Lowering the Voting Age This election year was very intense, many kids in my generation was interested in politics and was eager to vote. The excitement and anticipation of finding out the next president was one of those on the edge of the chair, biting your finger nail moments. I guess what I’m trying to say is that the voting age should be lowered. The voting age should be lowed to at least the age 16 or older. If you let a 16 year old teenager behind the wheel of the car then we should definitely be able to vote! Just like any other American, us young Americans would like for our voice to be heard too. "No right is more precious in a free country than that of having a choice in the election of those who make the laws under which ...we must live. Other rights, even the most basic, are illusory if the right to vote is undermined.” - Wesberry v. Saunders. So In this paper, I’ll answer the question WHY? Why should the voting age be lower? There are many pros and cons to this argument. Okay, adults consider us to young “responsible” adults. What they consider us to be and what they say we our which are “irresponsible kids” are totally different. There is research that shows that “young “responsible” adults” or “irresponsible kids” can make reasoned decisions, our voting age remains two years above most age restrictions. Sixteen year olds are mature enough to make important decisions such as voting. Our bodies are fully develop to be an adult, we have been educated for at least...
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...In Search of the Black Swan: Analysis of the Statistical Evidence of Electoral Fraud in Venezuela Ricardo Hausmann Harvard University Roberto Rigobón Massachusetts Institute of Technology September 3, 2004 *This study was requested by Súmate who also provided the databases we used. We appreciate the great information gathering effort carried out by this organization. We are equally indebted to a hard working collaborator who, because of institutional reasons, must remain anonymous. We thank Andrés Velasco as well for his useful comments. The opinions expressed in this report and the errors we may have incurred are our responsibility and do not compromise either Súmate, or the universities to which we belong. 1 Abstract This study analyzes diverse hypotheses of electronic fraud in the Recall Referendum celebrated in Venezuela on August 15, 2004. We define fraud as the difference between the elector’s intent, and the official vote tally. Our null hypothesis is that there was no fraud, and we attempt to search for evidence that will allow us to reject this hypothesis. We reject the hypothesis that fraud was committed by applying numerical maximums to machines in some precincts. Equally, we discard any hypothesis that implies altering some machines and not others, at each electoral precinct, because the variation patterns between machines at each precinct are normal. However, the statistical evidence is compatible with the occurrence of fraud that has affected every machine...
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...Cloning and expression of α-Amylase gene from Bacillus subtilis in Pichia pastoris and Escherichia coli. Introduction Enzyme is a type of catalyst that present in living organisms used for many biotechnological functions in various industrial processing. It has a special characteristic that allows the chemical reaction to speed up without being altered, thus significantly improve the industrial productivity (Roy et al. 2012). Among various enzymes available in market, α-amylase has received a special attention in commercial production due to its widely used applications. α-Amylase contributed to 50% of the world enzyme production and has a great importance in many industries such as in food processing, laundry and also in pharmaceutical (Asgher et al. 2007). α-Amylase enzyme acts on α-1,4 glycosidic bonds in starch substrate backbone leading to the formation of soluble maltodextrins, glucose and maltose (Vidyalakshmi et al. 2009). This characteristic is extremely useful especially in industries that require the hydroxylation of starch such as the production of sugar syrups. The α-amylase enzyme can be obtained from various sources such as plants animals and microbes (Ahmed et al. 2011). However, the naturally occurring enzyme is still insufficient to support all the industrial production and therefore, it is crucial to find a new alternative sources, which is cost-efficient and high yield capacity to meet the supply demand (Yin et al. 2003). In industries, the microbial...
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...traits are influenced by things like their genotype or their specific environment. Genomic markers can allow for a more direct comparison of closely related individuals (Ansari and Khan, 2012). In our case we focus on DNA extraction. The two basic parts of a DNA extraction procedure include the breaking of the cell walls to expose the DNA and the use of enzymes to remove contaminants. The DNA is analyzed for purity by taking the absorbance. The pure DNA is then visualized by gel electrophoresis. The DNA extraction of plant seeds is difficult because of their cell wall. The method used to break the cell wall includes grinding the seeds with liquid nitrogen. The addition of DNAzol is used to isolate genomic DNA (Chomczynski et al. 1997). Restriction enzymes are necessary to fragment patterns of the DNA and in turn making it easier to analyze the DNA through gel electrophoresis. BACKGROUND The purpose of our experiment is to extract the DNA from pepper seeds to be able to compare and contrast the similarities in their DNA. The extraction of DNA from a plant is slightly more difficult than that of mammals. This is due to the presence of a rigid cell wall. To overcome this problem, the pepper seeds are grinded using liquid nitrogen (Manen, Jean-François, 2005). The liquid nitrogen facilitates the pulverization of the seeds by flash freezing them and causes the outer shell to become fragile. Plant DNAzol is a guanidine thiocyanate and detergent...
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...never had a good relationship. Her jealously towards the victim was well known and they were seen in public one week prior to the incident arguing, which raises a red flag. Background The victim was a cheerleader in college and she was an honor student. She was murdered by multiple stabs and the guilty suspect disposed of the body in a dumpster near the library. Luckily, there was a trail of blood left at the scene and there was a blood sample that was connected to one of the suspects. The suspects narrowed down to her half sister who was very jealous of her and her ex-boyfriend. Similar to the procedure done in forensics the samples of the crime scene blood, the blood of suspect A and the blood of suspect B were combined with restriction enzymes and put through gel electrophoresis to determine which suspect’s blood matched the blood found at the crime scene. Results __Wide Range Marker / __Crime / / __Crime-Enz / / / __Suspect A / / / / __Suspect A-Enz / / / / / __Suspect B / / / / / / __Suspect B-Enz / / / / / / / __EL...
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... Nearly half of Americans come in contact with or personally know an individual who has anorexia. In fact, I am sure each one of us knows someone or knows of someone who has an eating disorder. My oldest sister has anorexia and I have sorrowfully watched her struggle daily with it. It happened gradually and without warning when she was only 12. She kept a journal of her daily calorie intake, and one of her entries read that she only had a small handful of nuts and bite of a brownie that day. Like my sister, anorexia is a growing problem among young women. Throughout this speech, you will learn of some aspects of this eating disorder such as its causes, preventions, and effects. Some signs and symptoms include the denial of hunger, withdrawal from usual activities and friends, refusal to eat certain foods or any food at all, and frequent comments about feeling “fat” even though dramatic weight loss is occurring. Many consequences happen as a result of Anorexia, such as an abnormally slow heart right and low blood pressure, reduction of bone density, muscle loss and weakness, severe dehydration which can lead to kidney failure, fainting and fatigue, dry hair and skin as well as hair loss, and the growth of a thin layer of hair covering the whole body called Lanugo, which is the body’s attempt to keep itself warm. Nearly 5% of all women worldwide suffer from Anorexia Nervosa, 90% of which are women between the ages of 15 and 24. Anorexia is a severe eating disorder characterized...
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...[Type the company name] | [Type the document title] | [Type the document subtitle] | | Monica Velez | [Pick the date] | [Type the abstract of the document here. The abstract is typically a short summary of the contents of the document. Type the abstract of the document here. The abstract is typically a short summary of the contents of the document.] | What is federalism? The federal principle or system of government. A question that many American individuals are asked and don’t have a general understanding of what it may mean. I think federalism is a great government Institution It makes difference in the state government agency. it's also self-sufficient government to every state In the United states, people choose every elected Official Government for equal Opportunity most of the society In the world are federalism it's very Important to distribute the wealth to the state and the local community, and much more. Since its inception more than 200 years ago, American federalism has undergone tremendous change. Today, all governments -- federal, state and local -- play a greater role in the lives of their citizens, expectations about what kind of services and rights people want from government have changed, and relations among the federal, state and local governments have become infinitely more complex. In this brief essay, Ellis Katz, professor of political science and a fellow of the Center for the Study of Federalism at Temple University, explores the origins...
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