Effect of Temperature on Rate of Reaction of Catalase

Abstract Properties of Enzymes focused on the variations of reaction rates amongst enzymes subjected to various circumstances such as temperature, pH levels, different concentrations of substrate, salt concentrations, Metal Copper Sulfate and lastly, the presence of an Enzyme Inhibitor. The assigned section of this laboratory for our efforts was the effect of temperature variations on enzyme reactions. To perform the experiment, we used a spectrophotometer to monitor the baseline catalase activity when they are placed in these two temperatures. In this way, absorbance can be measured over time to monitor catalase activity of the main baseline reaction. Our results showed that temperatures at higher degrees led to being inactive, whereas those at lower degrees lowered the reaction time. This comes to show that each enzyme can have a different optimal temperature and this experiment helped us to understand how reaction rate can be affected by temperature change.

Introduction Thousands of complex biological processes are constantly taking place within our bodies. We require material transport, energy synthesis, and the manufacturing of various proteins, hormones, and other molecules (Source 1). Almost all of these everyday processes rely on the function of enzymes to take place. Enzymes are specifically grouped according to their function, and this information can often provide us with clues regarding what type of reaction that enzyme will catalyze (Source 2). An enzyme can only react with a very specific type of substrate, which binds to the area called the active site. After the substrate binds to the active site, an enzyme-substrate complex is created, and then the products are released. The enzyme is never completely consumed from a reaction, and can be reused many times. Both active sites and substrates