Premium Essay

Yeast Culture Lab

In:

Submitted By amymo
Words 1353
Pages 6
Yeast Culture Lab

Introduction Yeast is a one-celled, microscopic organism, which is part of the fungi kingdom. Yeasts do not make up a single group (Smith & Smith, 2012). Yeasts use organic material as a means of making energy, which make them chemoorganotrophs (Smith & Smith, 2012). Carbon is procured primarily from hexose sugars, such as fructose and glucose. Yeast need either oxygen for aerobic cellular respiration or for species that are anaerobic, but also have aerobic methods creating energy (Smith & Smith, 2012). There are no species of yeast species that are known to grow only anaerobically. Yeasts thrive in an environment with a slightly acidic (Smith & Smith, 2012). The reproductive cycle of yeasts can be either asexual or sexual depending on the species. The most widely seen method of growth in yeast is asexual reproduction referred to as budding (Smith & Smith, 2012). Reproduction in reference to yeast depends on the species; the species can be both asexual by mitosis and sexual by budding (Smith & Smith, 2012). Consumption refers to use and the rate of use of something such as how a consumer, such as a primary consumer like a tree would use photosynthesis to make energy from carbon dioxide. Death in reference to a population is referring to the rate of death in that population (Smith & Smith, 2012).
Hypothesis
The primary goal of the yeast culture lab is to test a theory involving samples of yeast cultures grown in four conditions. The initial hypothesis is that yeast will undergo considerable growth in all environments but will show an increased rate of growth in a solution of sugar because yeast uses sugar as a source to generate energy naturally. The assumption is that this hypothesis is correct and that the yeast will grow quickly in each environment and,in particular, in the sugar solution.

Similar Documents

Free Essay

Yeast Culture Lab

...Yeast Culture Lab BIO/315 April 22, 2014 Annissa Furr Yeast Lab Yeast, is a microscopic one celled organism that belongs to the group of organisms that is called fungi. They are single celled organisms that have a scientific name of Saccharomyces cerevisiae. Yeast can have many purposes but the main purpose of yeast is to help the fermentation process. Yeast is a living organism known as a fungus and it gets its energy source from sugar. Yeast can also be used in creating certain medical intentions that heal wounds and reduce inflammation because of the large amount of hormones and enzymes. In reference to yeast reproduction depends on the type of species because they can be either asexual by mitosis or sexual by budding. Consumption talked about the use and rate of use of a primary consumer that needs photosynthesis in order to create energy from CO2. Death is in reference to a population and refers to the toll of death in a population. Hypothesis This labs main objective is to test cultures referring to yeast. The hypothesis is that the yeast will grow in all the environments that it is placed in but it will thrive in the environment that has sugar that is because yeast uses sugar as an energy sources naturally which will make the yeast generate more. The yeast could be identical in all the environments but most likely the situation will be that the yeast grows rapidly in the sugar. This hypothesis needs to be tested and in order to do that their...

Words: 1280 - Pages: 6

Premium Essay

Lab Paper

...Yeast Culture Lab I. Introduction 1. Yeasts are eukaryotic, unicellular fungi that inhabit liquid or moist habitats. They are heterotrophs and rely on complex organic substances for nutrition. Yeasts require oxygen for aerobic cellular respiration but some are also anaerobic with alternative aerobic methods for producing energy. They do not require light to grow and their temperature range varies which means they can survive in a multitude of different environments. Very common, they can be found anywhere, from occurring on the skins of fruits to residing in the intestinal tracts of animals as parasites. The most common method of reproduction for yeasts is asexual reproduction through budding. In this process, the parent cell’s nucleus splits and forms a daughter nucleus. The daughter cell grows on the parent cell until it is large enough to separate. Through cytokinesis, this ‘bud’ then forms a new cell. Less common is the method of sexual reproduction in which spores are formed. In this lab, I will be culturing four yeast samples under different conditions. The goal is to study the effects of limited reproduction, additional resources (nutrition) and predation on the yeast population. Yeasts play a very important role not only by serving as nutrient recyclers in nature, but by also being important for the food industry because they can convert carbohydrates to carbon dioxide and alcohols through fermentation. They are also used in cell biology research and produce ethanol...

Words: 1296 - Pages: 6

Premium Essay

Rates of Chemical Reaction

...affected by temperature. This experiment is done to test the effect of five different temperatures on the rate of carbon dioxide production in yeast by measuring the fermentation rate. “The fermentation rate is measured in ml/min.” The purpose for performing this experiment is to understand the rate of fermentation of yeast cells which can be determined by measuring the amount of carbon dioxide produced at each temperature. Since there is no direct way to measure the carbon dioxide produced in the lab, an indirect method is used instead. The fermentation rate in this experiment is measured in ml/min which is the rate of carbon dioxide production that is measured over time spent in each water bath. Water will substitute for the carbon dioxide produced at the end of the experiment. The amount of water is measured in milliliters (by the graduated cylinder) will serve as an indirect method of fermentation rate because carbon dioxide is a gas which cannot be measured by means other than indirectly with this experiment. Carbon dioxide production was measured by measuring the volume of water at different temperature; 25°C, 35°C, 45°C, 55°C, and 65°C. The time was also recorded at which all the measurements were taken for the different temperatures. Materials and Method The materials used in this experiment was: Yeast culture (contains yeast and glucose in the form of molasses) Five fermentation tubes, Water, Red Wax pencil, Graduated cylinder Water baths (labeled 25°C...

Words: 1074 - Pages: 5

Premium Essay

Bio156 Lab 5

...56 Define the following terms: Cellular respiration (aerobic respiration) (2 points) Cellular respiration is the process by which cells get their energy in the form of ATP. There are two types of cellular respiration, aerobic and anaerobic. Aerobic respiration is more efficient and can be used in the presence of oxygen. Aerobic respiration, or cell respiration using oxygen, uses the end product of glycolysis in the TCA cycle to produce more energy currency in the form of ATP than can be obtained from an anaerobic pathway. Fermentation (anaerobic respiration) (2 points) Fermentation is a metabolic process converting sugar to acids, gases or alcohol.  It occurs in yeast and bacteria, but also in oxygen-starved human muscle cells. Fermentation is used by humans to make beer and food, like kimchi. Fermentation is a form of anaerobic digestion that generates ATP by the process of substrate-level phosphorylation. Summarize what occurs during the three steps of cellular respiration and indicate where each process takes place in the cell. (6 points) Glycolysis: It is a process that occurs in the cytoplasm. It converts each molecule of glucose to two molecules of pyruvic acid. It refers to an anaerobic process that proceeds whether or not oxygen is present or not. The pyruvic acid diffuses into the inner compartment of the mitochondrion where a transition reaction  occurs that starts to prepare pyruvic acid for the next stage of respiration. Krebs cycle-This is...

Words: 964 - Pages: 4

Free Essay

Characterisation of the Antiyeast Compound

...Red de Revistas Científicas de América Latina, el Caribe, España y Portugal Sistema de Información Científica Duangporn Kantachote, Pakorn Prachyakij, Wilawan Charernjiratrakul, Metta Ongsakul, Yodsawee Duangjitcharoen, Chaiyavat Chaiyasut, Teruhiko Nitoda, Hiroshi Kanzaki Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Electronic Journal of Biotechnology, vol. 13, núm. 5, 2010, pp. 1-15, Pontificia Universidad Católica de Valparaíso Chile Available in: http://www.redalyc.org/articulo.oa?id=173318799002 Electronic Journal of Biotechnology, ISSN (Electronic Version): 0717-3458 edbiotec@ucv.cl Pontificia Universidad Católica de Valparaíso Chile How to cite Complete issue More information about this article Journal's homepage www.redalyc.org Non-Profit Academic Project, developed under the Open Acces Initiative Electronic Journal of Biotechnology ISSN: 0717-3458 http://www.ejbiotechnology.info DOI: 10.2225/vol13-issue5-fulltext-1 Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages Duangporn Kantachote1 1 · Pakorn Prachyakij1 · Wilawan Charernjiratrakul1 Metta Ongsakul · Yodsawee Duangjitcharoen2 · Chaiyavat Chaiyasut2 Teruhiko Nitoda3 · Hiroshi Kanzaki3 1 Department of Microbiology, Faculty of Science, Prince of Songkla University,...

Words: 7395 - Pages: 30

Free Essay

Micro Essay

...MicroBiology- MLT1 LabPaq / Published by: Hands-On Labs, Inc. sales@labpaq.com / www.LabPaq.com / Toll Free 866.206.0773 A Laboratory Manual of Small-Scale Experiments for the Independent Study of Microbiology 50-0222-MB-01 LabPaq® is a registered trademark of Hands-On Labs, Inc. (HOL). The LabPaq referenced in this manual is produced by Hands-On Labs, Inc. which holds and reserves all copyrights on the intellectual properties associated with the LabPaq’s unique design, assembly, and learning experiences. The laboratory manual included with a LabPaq is intended for the sole use by that LabPaq’s original purchaser and may not be reused without a LabPaq or by others without the specific written consent of HOL. No portion of any LabPaq manual’s materials may be reproduced, transmitted or distributed to others in any manner, nor may be downloaded to any public or privately shared systems or servers without the express written consent of HOL. No changes may be made in any LabPaq materials without the express written consent of HOL. HOL has invested years of research and development into these materials, reserves all rights related to them, and retains the right to impose substantial penalties for any misuse. Published by: Hands-On Labs, Inc. 3880 S. Windermere St. Englewood, CO 80110 Phone: Denver Area: 303-679-6252 Toll-free, Long-distance: 866-206-0773 www.LabPaq.com E-mail: info@LabPaq.com Printed...

Words: 31695 - Pages: 127

Free Essay

Dna Lab

...(auxotroph vs. prototroph, genes vs. alleles, diploid vs. haploid, complementation, segregation and independent assortment, meiosis vs. mitosis, genotype vs. phenotype) Develop and test scientific hypotheses Learn to work with a microscope, micropipettor, microcentrifuge and hemacytometer Learn about the genetics of metabolism How this project will be graded: 1. Your scores for Question Sets 1, 2, and 3 will be combined into a single grade. That grade will be included in your Lab Average for the course. Your Lab Average will be included in your final semester average as described in the syllabus for this course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 2. Your scores for Question Sets 4, 5, and 6 will be combined into a single grade. That grade will be included in your Lab Average for the course. (the deadline for the question submission will be determined in class depending on the progress of the lab) 3. You will write a formal lab report in a style and format suitable for submission to the journal Molecular & Cellular Biology. Your report will be included in your final semester...

Words: 4873 - Pages: 20

Free Essay

Anaerobic Yeast Fermentation for the Production of Ethanol in a Versatile Lab Fermentor

...Anaerobic yeast fermentation for the production of ethanol in a versatile lab fermentor Abstract Whether used for research or production, the versatile BioFlo® 310 fermentor from New Brunswick Scientific allows growth of a wide variety of aerobic and anaerobic microorganisms, including bacteria, plant, algae, fungi and yeast. Its advanced controller can regulate up to four vessels simultaneously, 120 process loops in all. Here we demonstrate one facet of its versatility—a technique for inducing ethanol production in yeast, by switching from an aerobic growth phase to an anaerobic steady-state culture. Introduction Saccharomyces cerevisiae is a model eukaryotic organism, often used in research because it is easy to manipulate and culture, and is comparatively similar in structure to human cells. This yeast is also widely used in industrial applications to manufacture enzymes and proteins for beer, wine and bread, and because it metabolizes glucose to ethanol, is also used to produce many biofuel products. We produced ethanol from a S. cerevisiae (American Type Culture Collection strain 20602) in a 7.5 liter BioFlo 310 fermentor, to demonstrate the flexibility of this advanced fermentation system. In the first phase, we grew the yeast in an aerobic environment, using a dissolved oxygen cascade control strategy to produce a sufficient cell density. Then we pumped in nitrogen gas to create an anaerobic environment for inducing ethanol production, and used reduction and oxidation...

Words: 984 - Pages: 4

Free Essay

Microbiology

...Name and course section: LeKesha Hinds Title: Bacterial Morphology- Lab #2 Purpose: Being able to observe morphologies by preparing wet mount slides and learning direct and indirect staining techniques. Procedure: First, set up the microscope. View the prepared slides of bacterial morphology. Use each morphological type as a comparative tool for the remainder of the exercise. Disinfect your work area with a 10%-bleach solution using the Procedures in the Preparation of Disinfecting Solution section in the Appendix. Use the marker pencil to make a dime-sized circle on each of the three slides. Use a clean pipet to add a drop of warm water to the circle on the first slide. With the cotton swab vigorously scrape the inside of your mouth and gums. Smear the swab inside the circle on the first slide, transferring as much material to the drop of water as possible. Cover the drop with a cover slip. Use the pipet to add a drop of water to the circle on the second slide. Use the toothpick to scrape a sample of plaque from your teeth. Transfer the plaque from the toothpick to the drop of water, mixing well to dissolve any clumps. Cover the drop with a cover slip. Use the pipet to add a drop of the S. cerevisiae mixture to the circle of the third slide. Cover the drop with a cover slip. Set the cup of yeast mixture and its pipet aside for later use. Second, do the same as for the first slides. When the slides are completely dry, heat-fix each slide with a flame source. Hold sample...

Words: 1347 - Pages: 6

Premium Essay

Note

...The Diversityof Life Lab Manual Stephen W. Ziser Department of Biology Pinnacle Campus for BIOL 1409 General Biology: The Diversity of Life Lab Activities, Homework & Lab Assignments 2013.8 Biol 1409: Diversity of Life – Lab Manual, Ziser, 2013.8 1 Biol 1409: Diversity of Life Ziser - Lab Manual Table of Contents 1. Overview of Semester Lab Activities Laboratory Activities . . . . . . . . . 2. Introduction to the Lab & Safety Information . . . . . 3 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 15 30 39 46 54 68 81 104 147 3. Laboratory Exercises Microscopy . . . . . . Taxonomy and Classification . Cells – The Basic Units of Life . Asexual & Sexual Reproduction Development & Life Cycles . . Ecosystems of Texas . . . . The Bacterial Kingdoms . . . The Protists . . . . . . The Fungi . . . . . . . The Plant Kingdom . . . . The Animal Kingdom . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 13 17 22 26 29 . 32 . 42 . 50 . 59 . 89 4. Lab Reports (to be turned in - deadline dates as announced) Taxonomy...

Words: 21204 - Pages: 85

Premium Essay

Type 2 Diabetes Case Study Essay

...Clinical presentations Diabetes Mellitus and in our case type 2 diabetes often presents itself less dramatically in patients than many other conditions . This characteristic of the disease often results in patients overlooking their symptoms as they tend to develop gradually. Patients affected with diabetes mellitus type 2 may often exhibit the classic signs and symptoms for a longer duration as well as those with most commonly associated with hyperglycemia such as polyuria ,polydipsia and weight loss - which is more often seen in type 1 diabetics. Whilst type 2 diabetics less often experience vague manifestations of their condition such fatigue, weight gain and frequent vaginal yeast infections. Further development of the DMT2 often results in changes that influence both the visual and sensational systems within the body. We were able to detect our patient Mrs. X's clinical presentations after subjecting her to a general examination and special investigations which included taking a blood...

Words: 1226 - Pages: 5

Free Essay

Iga Levels

...Microbiology Overview Interpretation of preliminary microbiology data Gram-positive cocci Aerobic In clusters ● Coagulase (+): Staphylococcus aureus ● Coagulase (-): Staphylococcus lugdunensis and other coagulasenegative staphylococci In pairs/chains ● Optochin sensitive: Streptococcus pneumoniae ● Alpha-hemolytic: Viridans group Streptococcus, Enterococcus ● Beta-hemolytic: ○ Group A Strep (Streptococcus pyogenes) ○ Group B Strep (Streptococcus agalactiae) ○ Group C, D, G Strep Anaerobic: Peptostreptococcus spp. and many others Gram-positive rods Aerobic ● Large: Bacillus spp ● Cocco-bacillus: Listeria monocytogenes, Lactobacillus spp ● Small, pleomorphic: Corynebacterium spp ● Branching filaments: Nocardia spp, Streptomyces spp Gram-negative cocci Aerobic ● Diplococcus: Neisseria meningitidis, N. gonorrhoeae, Moraxella catarrhalis ● Cocco-bacillus: Haemophilus influenzae, Acinetobacter Anaerobic: Veillonella spp. Gram-negative rods Aerobic Lactose fermenting (Lactose positive): ● Enterobacter spp, Escherichia coli, Klebsiella spp ● Citrobacter spp*, Serratia spp* Non lactose-fermenting (Lactose negative): ● Oxidase (-): Acinetobacter spp, Burkholderia spp, E. coli, Proteus spp, Salmonella spp, Shigella spp, Serratia spp*, Stenotrophomonas maltophilia ● Oxidase (+): P. aeruginosa, Aeromonas spp. Anaerobic ● Large: Clostridium spp Anaerobic: Bacteroides spp, Fusobacterium spp, Prevotella spp. ● Small, pleomorphic: P. acnes, Actinomyces spp *Serratia and Citrobacter spp...

Words: 3768 - Pages: 16

Free Essay

Swine Flu

...Exam 2 Essay Terrika Moore “Question 10 Answer” The Bergey’s Manual is a system used to classify procaryotes and bacteria. The first edition of the guide was based on specific characteristics of gram staining and metabolic reactions, which was a method called phenotypic. However with the new second edition, classifications and identification goes more in depth. It’s based on genetic information, specifies the relationship and phylogenetic history of the bacteria. Bergey’s Manual 2nd edition is broken down into five volumes: Vol. 1 separates Domain Archaea and Domain Bacteria into their own groups. Vol. 2 represents the Phylum Proteobacteria, breaks them into 5 classification groups. These bacteria all have gram negative cell walls. Vol. 3 represents Phylum Firmicutes, these are the low G + C gram positive bacteria which only has 3 classes. This group of bacteria includes staphylococcus and streptococcus. Vol. 4 includes a single class of Phylum Actinobacteria, in which this bacterium is a high G + C gram positive bacteria. Vol. 5 contains 9 Phyla in which they’re all gram negative but may or may not be related. “Question 7 Answer” Zygomycota are saprophytic molds in which this fungus receives it nourishments from dead or decaying matter. This fungus is the black mold on bread. Its asexual reproduction is sporangiospores and sexual spores are large zygospores and are enclosed by a thick wall. Zygomycota can cause serious infections in immunosuppressed or diabetic...

Words: 994 - Pages: 4

Free Essay

Task 2

...Task Stream 2 Microbiology can be broken down into categorizations based upon the environmental conditions necessary for organisms in which to grow. Two large categories of microorganisms are those requiring oxygen to live (obligate aerobes) and those which can grow with oxygen but have the ability to also grow without it (facultative aerobes). The obligate aerobes produce more energy from nutrients than anaerobes by using oxygen as the “final electron acceptor in the electron transport chain, which produces most of the ATP in these organisms”(Betsy & Keogh, 2005, p.104). The facultative microorganisms are able to use oxygen but can also go without by using fermentation or anaerobic respiration when it is not available (Betsy & Keogh,2005). The microorganisms being cultured in our first task (Lactobacillus acidophilus and Staphylococcus epidermidis) are obligate aerobes. Microorganisms can grow in a variety of conditions with temperature being one of those variables, but the types we frequently encounter in our environment thrive in fairly warm temperatures. Both Lactobacillus acidophilus and Staphylococcus epidermidis are examples of these, which are referred to as mesophiles. Extreme temperatures (as in deep freezing or auotoclaves for example) are effective in destroying microorganisms due to their inability to thrive outside of more moderate temperatures). Growth of these two organisms would be optimized by remaining between 25 and 40 degrees celsius (Betsy...

Words: 1281 - Pages: 6

Free Essay

Antifungal Activity of Star Anise (Illicium Verum) to Candida Tropicalis

...ANTIFUNGAL ACTIVITY OF STAR ANISE (Illicium verum) TO Candida tropicalis _______________ An Investigatory Project Presented to the Faculty Of Talamban National High School Talamban, Cebu City _______________ In Partial Fulfillment of the Requirements for Research I _______________ by Queenibel S. Arriesgado Kristine Jane A. Borces Dominique Fatima G. Cabansay Jobelyn B. Cogtas Mary Rose A. Telamo June 2014 APPROVAL SHEET The investigatory project entitled “ANTIFUNGAL ACTIVITY OF STAR ANISE SEEDS (Illicium verum) to Candida tropicalis”, prepared and submitted by Queenibel S. Arriesgado, Kristine Jane A. Borces, Dominique Fatima G.Cabansay, Jobelyn B. Cogtas, Mary Rose A. Telamo, in partial fulfillment of the requirements for Reasearch I, has been examined for acceptance and approval for Oral Examination. RESEARCH ADVISORY COMMITTEE MA. JESSICA N. ABAYON, Ed. D. Chairperson CELIA C. GEPITULAN, M. Ed. JOCELYN C. BUTANAS, M. Ed. Adviser Member CELIA C. GEPITULAN, M. Ed. FARAH C. CENIZA Member Member ------------------------------------------------- PANEL OF EXAMINERS Approved by the Committee of Oral Examination of with a grade...

Words: 4807 - Pages: 20