INTRODUCTION: Lipase also called as triacylglycerol acylhydrolaseis an enzyme known for its enormous applications for industry and diagnostics. Their basic activity is to convert fats into fatty acids and glycerol. These enzymes are water soluble in nature. They also convert polar solvents into more lipolytic substances. In 1856, a scientist name claude Bernard has identified lipase [1]. Lipases are serine hydrolases containing G-X1-S-X2-G sequences as the catalytic part of the particle, where G
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ABSTRACT Objective: Investigate the influence of ethanol, polyethylene glycol 400, propylene glycol, glycerol and sorbitol on the absorptive clearance of ketotifenfumarate in the rabbit. Methods:In situ intestinal perfusion technique, ̎through and through ̎ was used for estimation of membrane transport parameters of ketotifenfumarate from duodenum, jejunum, ileum and ascending colon in the rabbit. These parameters include absorptive clearance per unit length PeA/L (ml/min.cm), percentage fraction
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membrane (2 marks) -hydrophilic heads points outwards -hydrophobic tails inwards b. The role played by the RER in production of the enzyme (2 marks) -Ribosomes on surface -Ribosomes- site of Protein Synthesis -Ribosome provides high surface area for Protein synthesis c. The role played by the Golgi Apparatus in secretion of the enzyme (1 mark) -The enzyme is secreted as Vesicle (idea pinch off the membrane of golgi) and (travel to cell surface membrane) 2. Outline 2 factors which are
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Methyl Red Voges-Proskauer Test Lab Tetia Richardson Microbiology DL3: Instructor Newton 3/29/2015 PURPOSE This experiment is designed to become familiar with and perform the MR-VP biochemical test, learn variations in how different organisms metabolize glucose, and to become familiar with and perform the catalase biochemical test. Materials Used 10% bleach solution Hydrogen peroxide Paper towels Saved E. coli culture Stock culture: S. epidermidis Gloves Candle used for a flame
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Bioremediation Methodologies Summer Teeters COM/172 June 23, 2014 Sherry Howard Salois Bioremediation Methodologies What is bioremediation? According to the Gale Encyclopedia of Science (2008), bioremediation is any process that purifies an environment polluted by organic or inorganic contaminants with the utilization of microorganisms or other such creatures. Intensive industrialization and inadequate disposal of organic and inorganic compounds have brought about long-term persistent
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Affect Enzyme Activity Purpose The purpose of this lab is to see if changing the temperature, pH, or concentration of enzymes effects catalase activity. Background Enzymes are in every living thing. Enzymes are catalysts they speed up chemical reactions by reducing the amount of energy needed to perform them. Without them most chemical reactions would happen much too slow for life to exist. Enzymes are very special and each is designed to do one specific thing. One type of enzyme per type
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nerve signals from cell to cell. Some minerals help regulate water balance, others help regulate energy metabolism, and some affect growth and development through their role in the expression of certain genes. Many minerals act as cofactors needed for enzyme activity. None of the minerals we require acts in isolation. Instead, they interact with each other as well as with other nutrients and other components of the diet. Eating a variety of foods, including fresh fruits, vegetables, nuts, legumes, whole
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Abstract Without the effects of enzymes, many crucial chemical reactions would not be able to take place at the rate of which they were meant to perform. We conducted this series of labs in order to discover the effects of different biological and environmental aspects on enzymatic activity. In our first experiment, we looked at the effects that enzyme concentration had on amylase activity. We hypothesized that the higher the concentration of the enzyme, the higher the rate of reaction would
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Biology Chapter 1: Maintaining a balance Unit 1.1 Activity and Temperature Role of Enzymes * Metabolism – All of the chemical processes occurring within an organism * Chemical activity is maintained by large proteins – Enzymes * Enzymes: * Protein molecule that acts as a biological catalyst * Speed up rates of reactions * Their action is specific: catalyzing only one type of reaction * Used over and over in small quantities * Made in cell when they’re
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treatments were arranged to disaggregate the fibrous structure followed by enzyme treatments to breakdown cellulose polymers and to hydrolyse sucrose. The optimal time-temperature conditions of each process step were searched for gaining the highest reducing sugars yield at the end of the saccharification. Cellulolytic enzymes were tested for 4-8-18-24 h at 30-40-50 °C, invertase addition was arranged, and amylolytic enzymes were evaluated. All determinations were done in duplicate and a factorial
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