For the material; we had a box for housing flies, a lock to lock the locker containing the box for housing flies, group lab books, vials, foam plugs, fly-nap, netting, anesthetizing wand, media contain yeast and antifungal agent, plastic cups, paintbrushes, index cards, vial labels, record sheets, and Carolina Drosophila. Then we had to get ready the Drosophila fly vials; to finish these eight actions are essential. Phase one add 1.5 cm of flakes into vial to about 1.5 cm high of the vial. Phase
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Materials. Minimum essential medium alpha modification (α-MEM) with L-glutamine (2.0 mM), penicillin-streptomycin, trypsin ethylene diamine tretaacetic acid (EDTA), 6- thioguanine (6-TG) were purchased from Sigma-Aldrich. Fetal bovine serum (FBS) was purchased from Hyclone (Logan, UT). Dimethylsulfoxide (DMSO) and potassium chromate (K2CrO4) were purchased from Fisher Scientific. Reagents such as crystal violet, 70% ethanol were used as is. Cells were stored in liquid nitrogen until ready for use
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In the experiment “Enzyme Jelly Lab”, it was discovered that the hypothesis was rejected. The hypothesis being: If temperature affects enzyme function then fresh pineapple is the best to use when making jello. The rationale behind this hypothesis was that the bonds within the collagen of the fresh pineapple could withstand the temperature up to 70 degrees Celsius. This would allow bonds to repair themselves after cooling. When combined with hot water, the helices form a 3-D structure and is unraveled
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In microbiology lab different tests are performed for the diagnosis of different disease .they are used different techniques for the detection of presence of microorganism which are causing harmful disease Different test performed are • Test performed by ELISA technique a. Hepatitis B,C , b. Rheheumatoid arthritis (RF FACTOR ) c. Treponemapallidiumhaemagglutination test (TPHA) • Test performed by latex test a. CRP ( C reactive protein test ) b. ASO(anti - streptolysin o ) • Flocculation
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of Acid Phosphatase Activity Under a Variety of Environmental Conditions Introduction: Biological enzymes are very important as they allow the human body to function and produce a wide variety of biochemical reactions that help sustain life. The main purpose of this lab is to understand the narrow optimal range and varying perimeters that allow these enzymes to optimally function. The enzyme, acid phosphatase, and the substrate p-nitrophenylphosphate were subjected to several different conditions
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undergo co-translational glycosylation on selected asparagine residues. To unite the alpha and beta polypeptide chains, intrapolypeptide disulfide bonds form; these events result in the formation of a catalytically active enzyme. In order to target the lysosome, where the enzyme operates, phosphomannosyl recognition markers need to be generated. They are formed by the sequential action of a phosphotransferase that add UDP-N-acetylglucosamine to selected mannose residues and a phoshpdiesterase a-N-acetylglucosaminidase
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Introduction Tyrosinase is an enzyme that controls the production of melanin, which is a pigment found in plants, animals, bacteria and fungi. Both tyrosinase and melanin is found in nearly all cells. Melanin is especially important in determining the skin and hair color of mammalians. It is synthesized by an array of oxidative reactions of the amino acid tyrosine when tyrosinase is present (Kim, Y. & Uyama, H., 2005). Tyrosine is first hydroxylated into dihydroxyphenylalanine (DOPA), which is then
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Enzyme Post Lab Report Type of Fruit Prediction Result Apple None None Orange None None Pineapple Take longer to dissolve into the Jello Broke through the Jello quicker than the heated pineapple Heated Apple None None Heated Orange None None Heated Pineapple Breakdown through the Jell-O fast Broke through the Jell-O slower than the regular pineapple Q.1.) The piece of pineapple is the fruit that contains the enzyme Bromelain Q.2.) It is possible to make Jello with canned pineapple chunks but not
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specifically listing the criteria by which you believe a substance may be considered “natural” as opposed to “unnatural.” A: I would say that HFCS is “unnatural” because HFCS is produced by using an enzyme (Alpha-amylase) to break down corn-starch. This leads me to ask why must we need the enzyme if it were “nature” Q: Both “natural” and genetically modified (GM) forms of corn are available in the United States. Based on the criteria that you have listed for Question 2, does your answer to
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Unit One: Biochemistry Organic chemistry: chemistry of carbon based molecules * Life on our planet is carbon based * All life is carbon based, so to understand how biological processes occur we need to consider organic chemistry Hydrocarbons: molecules that are entirely made from carbon and hydrogen * Carbon you can get a lot of shape (rings etc) * Mostly non-metals (hydrogen, carbon, oxygen etc) make up molecules in our cells * Hydrogen, carbon, nitrogen, oxygen
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